Cited by 395Tuberculosis Research and EpidemiologyImmunotherapy and Immune ResponsesImmune responses and vaccinations

Apoptotic Vesicles Crossprime CD8 T Cells and Protect against Tuberculosis

Open access
Winau, Florian, Weber, Stephan, Sad, Subash, de Diego, Juana, Hoops, Silvia Locatelli, Breiden, Bernadette, Sandhoff, Konrad, Brinkmann, Volker +2 more·2006·Immunity

Abstract

CD8 T lymphocytes are important effectors in protective immunity against Mycobacterium tuberculosis. We recently characterized the detour pathway of CD8 T cell activation in tuberculosis mediated by apoptotic vesicles from infected cells that transport mycobacterial antigens to dendritic cells (DCs). Here we demonstrate that apoptotic vesicles from mycobacteria-infected macrophages stimulate CD8 T cells in vivo. Homing of DCs to draining lymph nodes was critically required for effective crosspriming. Subsequent fate of vesicle-associated antigens in recipient DCs was characterized by endosomal mechanisms predominating over proteasomal processing. In addition, vesicle processing depended on the presence of saposins to disintegrate apoptotic membranes. Apoptotic vesicles displayed potent adjuvant activity by stimulating through Toll-like receptors (TLR). Ultimately, vaccination with vesicles from infected cells induced protection against M. tuberculosis infection. Taken together, we propose the detour pathway to represent a genuine immunological mechanism mediating crosspriming of CD8 T cells in vivo and protection against tuberculosis.

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Crossref · 10.1016/j.immuni.2005.12.001

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  • Apoptotic blebs from infected macrophages can promote cross presentation and protective T-cell immunity.

    “As reported previously, apoptotic blebs from infected MΦs can also promote cross presentation and protective T-cell immunity (137, 157).”

    From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [137]

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Authors
Winau, F., Weber, S., Sad, S., de Diego, J., Hoops, S. L., Breiden, B., Sandhoff, K., Brinkmann, V., Kaufmann, S. H. E., & Schaible, U. E.
Affiliations
  • Max Planck Institute for Infection Biology (DE)
  • Institute for Biological Sciences (CA)
Corresponding author
Winau, Florian
Venue
Immunity
Publisher
Elsevier BV
Published
2006-01
Volume
24
Issue
1
Pages
105-117
ISSN
1074-7613 (ISSN-L)
OpenAlex
W2165948328
Cited by
395
References
53
Citations by year
2026: 6 · 2025: 9 · 2024: 19 · 2023: 8 · 2022: 16 · 2021: 13 · 2020: 14 · 2019: 12 · 2018: 14 · 2017: 15 · 2016: 15 · 2015: 17 · 2014: 31 · 2013: 25 · 2012: 22
FWCI
23.17
Type
journal article
Language
en
Open access
Locations
  • Immunity (publishedVersion)
  • PubMed (publishedVersion)
  • Max Planck Institute for Plasma Physics (submittedVersion)
  • LSHTM Research Online (London School of Hygiene and Tropical Medicine) (submittedVersion)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Immunology and Microbiology › Immunology › Immune responses and vaccinations
Keywords
apoptotic vesicles, tuberculosis, Mycobacterium tuberculosis, dendritic cells, macrophages, antigen transport, saposins, Toll-like receptors, adjuvant effect, draining lymph nodes, protective immunity
MeSH terms
Adjuvants, Immunologic, Animals, BCG Vaccine, Dendritic Cells, Macrophages, Mycobacterium bovis, Endosomes, Tuberculosis, Vaccination, Apoptosis, CD8-Positive T-Lymphocytes, Cross-Priming, Saposins, Toll-Like Receptors, Mice

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