The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.
The Transcription Factor PU.1 Controls Dendritic Cell Development and Flt3 Cytokine Receptor Expression in a Dose-Dependent Manner
Open accessAbstract
The transcription factor PU.1 plays multiple context and concentration dependent roles in lymphoid and myeloid cell development. Here we showed that PU.1 (encoded by Sfpi1) was essential for dendritic cell (DC) development in vivo and that conditional ablation of PU.1 in defined precursors, including the common DC progenitor, blocked Flt3 ligand-induced DC generation in vitro. PU.1 was also required for the parallel granulocyte-macrophage colony stimulating factor-induced DC pathway from early hematopoietic progenitors. Molecular studies demonstrated that PU.1 directly regulated Flt3 in a concentration-dependent manner, as Sfpi1(+/-) cells displayed reduced expression of Flt3 and impaired DC formation. These studies identify PU.1 as a critical regulator of both conventional and plasmacytoid DC development and provide one mechanism how altered PU.1 concentration can have profound functional consequences for hematopoietic cell development.
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“PU.1 is directly required to maintain the expression of the cytokine receptor Flt3 that is indispensable for progenitors with B and T cell potentials (73).”
From Mechanisms of Action of Hematopoietic Transcription Factor PU.1 in Initiation of T-Cell Development (2019) · cites this paper as [73]
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Inaba, K., Inaba, M., Romani, N., Aya, H., Deguchi, M., Ikehara, S., Muramatsu, S., & Steinman, R. M. (1992) · The Journal of Experimental Medicine · doi:10.1084/jem.176.6.1693 · Cited by 3,741
Polycomb complexes repress developmental regulators in murine embryonic stem cells
Boyer, L. A., Plath, K., Zeitlinger, J., Brambrink, T., Medeiros, L. A., Lee, T. I., Levine, S. S., Wernig, M., Tajonar, A., Ray, M. K., et al. (2006) · Nature · doi:10.1038/nature04733 · Cited by 2,568
Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages
Scott, E. W., Simon, M. C., Anastasi, J., & Singh, H. (1994) · Science · doi:10.1126/science.8079170 · Cited by 1,532
Hayashi, S., & McMahon, A. P. (2002) · Developmental Biology · doi:10.1006/dbio.2002.0597 · Cited by 1,351
Identification of Flt3+ Lympho-Myeloid Stem Cells Lacking Erythro-Megakaryocytic Potential
Adolfsson, J., Månsson, R., Buza-Vidas, N., Hultquist, A., Liuba, K., Jensen, C. T., Bryder, D., Yang, L., Borge, O. J., Thorén, L., et al. (2005) · Cell · doi:10.1016/j.cell.2005.02.013 · Cited by 1,323
Steady-state and inflammatory dendritic-cell development
Shortman, K., & Naik, S. H. (2006) · Nature reviews. Immunology · doi:10.1038/nri1996 · Cited by 1,212
Commitment to the B-lymphoid lineage depends on the transcription factor Pax5
Nutt, S. L., Heavey, B., Rolink, A. G., & Busslinger, M. (1999) · Nature · doi:10.1038/44076 · Cited by 1,179
Self-citationTargeted disruption of the PU.1 gene results in multiple hematopoietic abnormalities
McKercher, S. R., Torbett, B. E., Anderson, K. L., Henkel, G. W., Vestal, D. J., Baribault, H., Klemsz, M., Feeney, A. J., Wu, G. E., Paige, C. J., et al. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00949.x · Cited by 1,130
Maraskovsky, E., Brasel, K., Teepe, M., Roux, E. R., Lyman, S. D., Shortman, K., & McKenna, H. J. (1996) · The Journal of Experimental Medicine · doi:10.1084/jem.184.5.1953 · Cited by 1,117
In Vivo Analysis of Dendritic Cell Development and Homeostasis
Liu, K., Victora, G. D., Schwickert, T. A., Guermonprez, P., Meredith, M. M., Yao, K.-H., Chu, F.-F., Randolph, G. J., Rudensky, A. Y., & Nussenzweig, M. C. (2009) · Science · doi:10.1126/science.1170540 · Cited by 1,001
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Merad, M., Sathe, P., Helft, J., Miller, J., & Mortha, A. (2013) · Annual Review of Immunology · doi:10.1146/annurev-immunol-020711-074950 · Cited by 2,309
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The STAT3-Binding Long Noncoding RNA lnc-DC Controls Human Dendritic Cell Differentiation
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Deciphering the transcriptional network of the dendritic cell lineage
Miller, J. C., Brown, B. D., Shay, T., Gautier, E. L., Jojic, V., Cohain, A. T., Pandey, G. S., Leboeuf, M., Elpek, K. G., Helft, J., et al. (2012) · Nature Immunology · doi:10.1038/ni.2370 · Cited by 802
Metadata
- Authors
- Carotta, S., Dakic, A., D'Amico, A., Pang, S. H. M., Greig, K. T., Nutt, S. L., & Wu, L.
- Affiliations
- Walter and Eliza Hall Institute of Medical Research (AU)
- The University of Melbourne (AU)
- Corresponding author
- Nutt, Stephen L.
- Wu, Li
- Venue
- Immunity
- Publisher
- Elsevier BV
- Published
- 2010-05
- Volume
- 32
- Issue
- 5
- Pages
- 628-641
- ISSN
- 1074-7613 (ISSN-L)
- OpenAlex
- W1963617750
- Cited by
- 279
- References
- 53
- Citations by year
- 2026: 12 · 2025: 4 · 2024: 14 · 2023: 8 · 2022: 10 · 2021: 13 · 2020: 19 · 2019: 15 · 2018: 16 · 2017: 18 · 2016: 15 · 2015: 23 · 2014: 22 · 2013: 29 · 2012: 27
- FWCI
- 12.38
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 1209 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Immunity (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Leukaemia Foundation
- University of Melbourne
- Australian Research Council
- National Health and Medical Research Council
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- PU.1, dendritic cell development, FLT3, Flt3 ligand, plasmacytoid dendritic cells, myeloid dendritic cells, GM-CSF, transcriptional regulation, dose-dependent gene expression, conditional gene deletion, hematopoietic progenitor cells
- MeSH terms
- Proto-Oncogene Protein Spi-1, Animals, Base Sequence, Cell Differentiation, Cells, Cultured, Dendritic Cells, Dose-Response Relationship, Drug, Flow Cytometry, Membrane Proteins, Mice, Transgenic, Molecular Sequence Data, Promoter Regions, Genetic, Proto-Oncogene Proteins, Trans-Activators, Polymerase Chain Reaction, Models, Immunological, Mice
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