Ultrasound-assisted enzymatic extraction (UAEE) of phytochemical compounds from mulberry (Morus nigra) must and optimization study using response surface methodology
Abstract
A new method of ultrasound-assisted enzymatic extraction (UAEE) was applied to mulberry must treatment with processing variables of sonication frequency (FS), enzyme concentration (CE) and maceration time (TM) at constant temperature 20°C and pulse durations of 10s on and 5s off. A Box–Behnken Design (BBD) was used to investigate the main effect of the various variables on total phenolics (TPC), total flavonoids (TFC), total anthocyanins (TAC), lightest (L*) and Browning index (BI). The predicted model was found to be significant (p<0.05) with low standard error and high coefficients of determination (R2). The model was highly correlated to experimental results and this helped in the identification of the optimum operating conditions: FS=33.82kHz, CE=0.010% (v/w) and TM=11.58min, under which it predicted TPC=298.06mg/100ml; TFC=379.24mg/100ml; TAC=55.14mg/100ml; L*=3.51 and BI=1.19. The study shows that the UAEE could be employed to enhance the quality of must, reduce the time during the maceration process of juice or wine.
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Tchabo et al. [134] observed a substantial improvement in yield of UEAE compared with UAE alone and EAE alone.
“Also, Tchabo et al. [134] observed a substantial improvement in the yield of UEAE compared with UAE alone and EAE alone.”
From A comprehensive review of ultrasonic assisted extraction (UAE) for bioactive components: Principles, advantages, equipment, and combined technologies (2023) · cites this paper as [134]
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“Also, Tchabo et al. [134] observed a substantial improvement in the yield of UEAE compared with UAE alone and EAE alone.”
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- Authors
- Tchabo, W., Ma, Y., Engmann, F. N., & Zhang, H.
- Affiliations
- Jiangsu University (CN)
- Corresponding author
- Ma, Yongkun
- Venue
- Industrial Crops and Products
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 63
- Pages
- 214-225
- ISSN
- 0926-6690 (ISSN-L)
- OpenAlex
- W2035337206
- Cited by
- 105
- References
- 60
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 11 · 2023: 10 · 2022: 8 · 2021: 12 · 2020: 10 · 2019: 14 · 2018: 5 · 2017: 14 · 2016: 3 · 2015: 3
- FWCI
- 4.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Industrial Crops and Products (publishedVersion)
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Ziziphus Jujuba Studies and Applications
- Keywords
- ultrasound-assisted enzymatic extraction, Morus nigra, sonication frequency, enzyme concentration, maceration time, total phenolic content, total flavonoid content, total anthocyanins, browning index, juice color, extraction optimization
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