Ultrasound bath-assisted extraction of essential oils from clove using central composite design
Abstract
Ultrasound-assisted extraction of essential oils from clove was carried out using central composite design (CCD). The extraction parameters were optimized with response surface methodology (RSM). Three independent variables were extraction temperatures (ranging from 32 to 52°C), extraction times (ranging from 30 to 60min), and plant concentrations (ranging from 3 to 7%). The dependent (response) variable was clove extract. In this study, all the experiments were carried out in an ultrasound bath with a frequency of 53 kHZ. A high coefficient of correlation (0.94) was obtained between the predicted and actual clove extract yields. This result demonstrates the validity of the model used in the experiment. The statistical results showed that the extraction temperature had the most significant influence on the clove extract yield. The clove extract contained the following compounds: eugenol, α-caryophyllene, and 2-methoxy-4-(2-propenyl) phenol acetate. The major compound in the clove extract was eugenol. Antibacterial studies showed that essential oils derived from the ultrasound extraction of clove may be used as alternative bactericidal and bacteriostatic agents in the pharmaceutical industry.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Acoustic power and wave frequencies applied in liquid media can produce acoustic cavitation, where the creation, expansion, and implosion of bubbles enhance the selectivity of target molecules.
“Acoustic power and wave frequencies applied in liquid media can produce the acoustic cavitation phenomenon, where the creation, expansion, and implosion of bubbles enhance the selectivity of target molecules [12,53,54].”
From Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health (2021) · cites this paper as [54]
Organic extract obtained by ultrasound-assisted extraction (53 kHz) had significant eugenol, α-Caryophyllene, and eugenyl acetate contents.
“Tekin et al. [54] reported that organic extract obtained by UAE (53 kHz) had significant eugenol, α-Caryophyllene, and eugenyl acetate contents [54].”
From Clove essential oil (Syzygium aromaticum L. Myrtaceae): Extraction, chemical composition, food applications, and essential bioactivity for human health (2021) · cites this paper as [54]
Cites
Response Surface Methodology: Process and Product Optimization Using Designed Experiments
Gunst, R. F., Myers, R. H., & Montgomery, D. C. (1996) · Technometrics · doi:10.2307/1270613 · Cited by 9,576 · see citing works
Wiegand, I., Hilpert, K., & Hancock, R. E. W. (2008) · Nature Protocols · doi:10.1038/nprot.2007.521 · Cited by 6,156 · see citing works
Response surface methodology (RSM) as a tool for optimization in analytical chemistry
Bezerra, M. A., Santelli, R. E., Oliveira, E. P., Villar, L. S., & Escaleira, L. A. (2008) · Talanta · doi:10.1016/j.talanta.2008.05.019 · Cited by 6,075 · see citing works
Screening methods to determine antibacterial activity of natural products
Valgas, C., Souza, S. M. D., Smânia, E. F. A., & Smânia Jr., A. (2007) · Brazilian Journal of Microbiology · doi:10.1590/s1517-83822007000200034 · Cited by 1,403 · see citing works
Devi, K. P., Nisha, S. A., Sakthivel, R., & Pandian, S. K. (2010) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2010.04.025 · Cited by 908 · see citing works
Experimental design in chemistry: A tutorial
Leardi, R. (2009) · Analytica Chimica Acta · doi:10.1016/j.aca.2009.06.015 · Cited by 769 · see citing works
Lambert, R. J. W., & Pearson, J. (2000) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2000.01017.x · Cited by 528 · see citing works
Chemical Composition and Antioxidant Properties of Clove Leaf Essential Oil
Jirovetz, L., Buchbauer, G., Стоилова, И., Stoyanova, A., Krastanov, A. I., & Schmidt, E. (2006) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf060608c · Cited by 510 · see citing works
GUAN, W., LI, S., YAN, R., TANG, S., & QUAN, C. (2007) · Food Chemistry · doi:10.1016/j.foodchem.2006.04.009 · Cited by 466 · see citing works
Pei, R., Zhou, F. L., Ji, B., & Xu, J. (2009) · Journal of Food Science · doi:10.1111/j.1750-3841.2009.01287.x · Cited by 418 · see citing works
Şahin, S., & Şamlı, R. (2012) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2012.07.029 · Cited by 393 · see citing works
Kimbaris, A. C., Siatis, N. G., Daferera, D. J., Tarantilis, P. A., Pappas, C. S., & Polissiou, M. G. (2006) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2004.12.003 · Cited by 281 · see citing works
Minjares-Fuentes, R., Femenia, A., Garau, M. C., Meza-Velázquez, J. A., Simal, S., & Rosselló, C. (2014) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2014.02.013 · Cited by 274 · see citing works
Optimization of ultrasound-assisted extraction of polyphenols from spruce wood bark
Ghitescu, R.-E., Volf, I., Cărăușu, C., Bühlmann, A.-M., Gilca, I. A., & Popa, V. (2014) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2014.07.013 · Cited by 259 · see citing works
Ramić, M., Vidović, S. S., Zeković, Z. P., Vladić, J., Cvejin, A., & Pavlić, B. (2014) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2014.10.002 · Cited by 209 · see citing works
Response surface optimization of ultrasound-assisted oil extraction from autoclaved almond powder
Zhang, Q., Zhang, Z., Yue, X., et al. (2009) · Food Chemistry · doi:10.1016/j.foodchem.2009.02.071 · Cited by 202 · see citing works
Faleiro, M. L., Miguel, G., Gomes, S. M., Costa, L. A., Venâncio, F., Teixeira, A., Figueiredo, A. C., Barroso, J. G., & Pedro, L. G. (2005) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf0510079 · Cited by 176 · see citing works
Zhu, C., Zhai, X., Li, L.-Q., Wu, X., & Li, B. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.01.022 · Cited by 173 · see citing works
A comparative study of various oil extraction techniques from plants
Danlami, J. M., Arsad, A., Zaini, M. A. A., & Sulaiman, H. (2014) · Reviews in Chemical Engineering · doi:10.1515/revce-2013-0038 · Cited by 159 · see citing works
Ultrasound-assisted extraction of polyphenols from native plants in the Mexican desert
Wong‐Paz, J. E., Márquez, D. B. M., Martínez-Ávila, G. C. G., Belmares, R. E., & Aguilar, C. N. (2014) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2014.06.001 · Cited by 132 · see citing works
Eugenol Reduces the Expression of Virulence-Related Exoproteins in Staphylococcus aureus
Qiu, J., Feng, H., Lü, J., Xiang, H., Wang, D., Dong, J., Wang, J., Wang, X., Liu, J., & Deng, X. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.00704-10 · Cited by 128 · see citing works
Supercritical extraction of clove bud essential oil: isolation and mathematical modeling
Reverchon, E., & Marrone, C. (1997) · Chemical Engineering Science · doi:10.1016/s0009-2509(97)00172-3 · Cited by 119 · see citing works
Roldán-Gutiérrez, J. M., Ruiz‐Jiménez, J., & LUQUEDECASTRO, M. (2008) · Talanta · doi:10.1016/j.talanta.2008.01.057 · Cited by 109 · see citing works
Alexandru, L., Cravotto, G., Giordana, L., Binello, A. E., & Chemat, F. (2013) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2013.07.011 · Cited by 89 · see citing works
Extraction of clove using pressurized hot water
Rovio, S., Hartonen, K. M., Holm, Y., Hiltunen, R., & Riekkola, M. (1999) · Flavour and Fragrance Journal · doi:10.1002/(sici)1099-1026(199911/12)14:6 · Cited by 79 · see citing works
Apolónio, J. D., Faleiro, M. L., Miguel, M. G., et al. (2014) · FEMS Microbiology Letters · doi:10.1111/1574-6968.12440 · Cited by 73 · see citing works
Majd, M. H., Rajaei, A., Bashi, D. S., Mortazavi, S. A., & Bolourian, S. (2014) · Industrial Crops and Products · doi:10.1016/j.indcrop.2014.03.031 · Cited by 71 · see citing works
A comparison of the extraction of clove buds with supercritical carbon dioxide and superheated water
Clifford, A. A., Basile, A., & Al‐Saidi, S. (1999) · Fresenius Journal of Analytical Chemistry · doi:10.1007/s002160051400 · Cited by 61 · see citing works
Optimized Ultrasound-Assisted Extraction of Phenolic Compounds from Polygonum cuspidatum
Kuo, C., Chen, B.-Y., Liu, Y., Chang, C. J., Deng, T., Chen, J., & Shieh, C. (2013) · Molecules · doi:10.3390/molecules19010067 · Cited by 57 · see citing works
Fang, X., Wang, J., Wang, Y., Li, X., Zhou, H., & Zhu, L. (2014) · Separation and Purification Technology · doi:10.1016/j.seppur.2014.10.007 · Cited by 53 · see citing works
Essential Oils from Six Herbal Plants for Biocontrol of the Maize Weevil
Kerdchoechuen, O., Laohakunjit, N., Singkornard, S., et al. (2010) · HortScience · doi:10.21273/hortsci.45.4.592 · Cited by 40 · see citing works
Supercritical ethanol extraction of bio-oils from German beech wood: Design of experiments
Akalın, M. K., Karagöz, S., & Akyüz, M. (2013) · Industrial Crops and Products · doi:10.1016/j.indcrop.2013.06.036 · Cited by 38 · see citing works
Self-citationKannan, N., Rajakumar, A., & Rengasamy, G. (2004) · Environmental Technology · doi:10.1080/09593330.2004.9619342 · Cited by 34 · see citing works
Scopel, R., Falcão, M. A., Lucas, A. M., et al. (2014) · The Journal of Supercritical Fluids · doi:10.1016/j.supflu.2014.06.003 · Cited by 32 · see citing works
Sage oil extraction and optimization by response surface methodology
Akalın, M. K., Tekin, K., Akyüz, M., et al. (2015) · Industrial Crops and Products · doi:10.1016/j.indcrop.2015.08.005 · Cited by 31 · see citing works
Self-citationTekin, K., Akalın, M. K., & Karagöz, S. (2015) · Clean Technologies and Environmental Policy · doi:10.1007/s10098-015-1021-y · Cited by 15 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Shen, L., Pang, S., Zhong, M., Sun, Y., Qayum, A., Liu, Y., Rashid, A., Xu, B., Liang, Q., Ma, H., et al. (2023) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2023.106646 · Cited by 892 · see citing works
“Tekin et al. [71] performed an extraction of essential oils from cloves, employing variables such as temperature, extraction time, and plant concentration.”
BackgroundHaro-González, J. N., Castillo-Herrera, G. A., Martínez-Velázquez, M., & Espinosa-Andrews, H. (2021) · Molecules · doi:10.3390/molecules26216387 · Cited by 463 · see citing works
Essential oil eugenol: Sources, extraction techniques and nutraceutical perspectives
Khalil, A. A., Rahman, U. U., Khan, M. R., Sahar, A., Mehmood, T., & Khan, M. (2017) · RSC Advances · doi:10.1039/c7ra04803c · Cited by 407 · see citing works
Khadhraoui, B., Ummat, V., Tiwari, B. K., Fabiano-Tixier, A. S., & Chemat, F. (2021) · Ultrasonics Sonochemistry · doi:10.1016/j.ultsonch.2021.105625 · Cited by 269 · see citing works
Wei, M.-C., Xiao, J., & Yang, Y.-C. (2016) · Food Chemistry · doi:10.1016/j.foodchem.2016.04.076 · Cited by 87 · see citing works
González-Rivera, J., Spepi, A., Ferrari, C., Duce, C., Longo, I., Falconieri, D., Piras, A., & Tinè, M. R. (2016) · Green Chemistry · doi:10.1039/c6gc02200f · Cited by 69 · see citing works
Movahhedi, F., Maghsodi, A., & Adlnasab, L. (2020) · Chemical Papers · doi:10.1007/s11696-020-01149-7 · Cited by 12 · see citing works
- Authors
- Tekin, K., Akalın, M. K., & Şeker, M. G.
- Affiliations
- Karabük University (TR)
- Gebze Technical University (TR)
- Corresponding author
- Tekin, Kubilay
- Venue
- Industrial Crops and Products
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 77
- Pages
- 954-960
- ISSN
- 0926-6690 (ISSN-L)
- OpenAlex
- W1843794758
- Cited by
- 110
- References
- 36
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 16 · 2023: 12 · 2022: 12 · 2021: 21 · 2020: 7 · 2019: 8 · 2018: 8 · 2017: 10 · 2016: 4
- FWCI
- 9.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Industrial Crops and Products (publishedVersion)
- Funders
- Utah Agricultural Experiment Station
- Karabük Üniversitesi
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
- Life Sciences › Agricultural and Biological Sciences › Food Science › Agricultural and Biological Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
- Keywords
- ultrasound-assisted extraction, essential oils, Syzygium aromaticum, extraction optimization, eugenol, α-humulene, antibacterial activity, extraction temperature, response surface methodology
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
