Cecropia obtusa, an Amazonian ethanolic extract, exhibits photochemoprotective effect in vitro and balances the redox cellular state in response to UV radiation
Abstract
In order to exhibit photochemoprotective effect a cosmetic formulation is supposed to display both the ability to absorb UVA/UVB radiation and exhibit a biological effect to protect the skin against the damage caused by the radiation. Because animal tests are no longer acceptable for cosmetic development, the present study evaluated the photochemoprotective potential of Cecropia obtusa leaf extract against UVA-induced damage in keratinocytes and explored, in detail, the antioxidant capacity using in vitro systems. The extract exhibited a high antioxidant capacity, particularly towards superoxide radical and singlet oxygen. The EC50 values for DPPH, superoxide radical and singlet oxygen were 1.63μg/mL, 0.34μg/mL and 0.55μg/mL, respectively. In addition, the total polyphenol content and total flavonoid content were 371.5mg of gallic acid equivalent/g and 66.68mg of quercetin equivalent/g, respectively. Through the cell model, the extract was able to protect the UVA radiation-induced damage. For lipid peroxidation and superoxide dismutase activity, the group that was treated for 24h and irradiated in the absence of the extract exhibited better protection than the group that underwent the same treatment and was irradiated in the presence of the extract. The opposite pattern was observed for the reduced glutathione levels, catalase activity and reactive oxygen species formation. In addition to protecting keratinocytes from UVA-induced damage and balancing the redox cellular state,…
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Extracts of Lippia sericea, Cecropia obtusa, Acacia catechu, Brazilian bamboo, and Lasallia pustulata have high SPF values in formulations (SPF = 7-86), with Brazilian bamboo most promising at SPF 44 after irradiation.
“The other extracts revealed to possess a complex mixture of compounds, namely a high content of phenols [102,105] and polyphenols [103] and high SPF values in formulations (SPF = 7–86) [102,103,104,105,106], being the extract of Brazilian bamboo species the most promising with a SPF value of 44, after irradiation [105].”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
A greater number of phenolic constituents in extracts is related to antioxidant activity.
“Furthermore, a relation between the presence of a greater number of phenolic constituents in the extracts and the antioxidant activity was detected [102,103,104].”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
Cecropia obtusa leaves extract has DPPH, superoxide, and singlet oxygen scavenging capacity (IC50 1.63, 0.34, 0.55 μg/mL) and maintains ROS equilibrium in HaCaT cells.
“Regarding leaves extract of Cecropia obtusa, it was demonstrated promising DPPH radical, superoxide radical, and singlet oxygen scavenging capacity (IC50 values of 1.63 µg/mL, 0.34 µg/mL, and 0.55 µg/mL, respectively), and the ability of maintaining the reactive oxygen species (ROS) species’ equilibrium in HaCaT cells [103].”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
Ethanolic extract of Amazonian Cecropia obtusa leaves has a sun protection factor (SPF) of 16.
“effect of C. obtusa leaves extract at 20 µg/mL (SPF = 16) without cytotoxic effect in keratinocyte HaCaT cell line [103].”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
Ethanolic extract of Amazonian Cecropia obtusa leaves at 20 μg/mL has no cytotoxic effect in the HaCaT keratinocyte cell line.
“effect of C. obtusa leaves extract at 20 µg/mL (SPF = 16) without cytotoxic effect in keratinocyte HaCaT cell line [103].”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
C. obtusa leaves extract at 20 μg/mL has SPF 16 without cytotoxic effect in keratinocyte HaCaT cell line.
“Alves et al. (2016) also proved the photoprotective effect of C. obtusa leaves extract at 20 µg/mL (SPF = 16) without cytotoxic effect in keratinocyte HaCaT cell line [103].”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
Extracts of Lippia sericea, Cecropia obtusa, Acacia catechu heartwood, Brazilian bamboo species, and Lasallia pustulata have antioxidant and photoprotective activities.
“Extracts of Brazilian Lippia sericea [102], Amazonian Cecropia obtusa [103], Acacia catechu heartwood [104], Brazilian bamboo species [105], and Lasallia pustulata [106] were also reported for their antioxidant and photoprotective activities.”
From UV Filters: Challenges and Prospects (2022) · cites this paper as [103]
Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302 · see citing works
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999) · Methods in Enzymology · doi:10.1016/s0076-6879(99)99017-1 · Cited by 19,125 · see citing works
Antioxidant Determinations by the Use of a Stable Free Radical
Blois, M. S. (1958) · Nature · doi:10.1038/1811199a0 · Cited by 13,075 · see citing works
McCord, J. M., & Fridovich, I. (1969) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)63504-5 · Cited by 12,952 · see citing works
A fluorometric method for determination of oxidized and reduced glutathione in tissues
Hissin, P. J., & Hilf, R. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90326-2 · Cited by 4,116 · see citing works
Handbook of methods for oxygen radical research
Greenwald, R. A. (1987) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(87)80012-6 · Cited by 2,384 · see citing works
Neutral red uptake assay for the estimation of cell viability/cytotoxicity
Repetto, G., del Peso, A., & Zurita, J. L. (2008) · Nature Protocols · doi:10.1038/nprot.2008.75 · Cited by 2,217 · see citing works
Oxidation events and skin aging
Kammeyer, A., & Luiten, R. M. (2015) · Ageing Research Reviews · doi:10.1016/j.arr.2015.01.001 · Cited by 980 · see citing works
López‐Alarcón, C., & Denicola, A. (2012) · Analytica Chimica Acta · doi:10.1016/j.aca.2012.11.051 · Cited by 782 · see citing works
Optimization of extraction of phenolics from Inga edulis leaves using response surface methodology
da Silva, E. M., Rogez, H. L. G., & Larondelle, Y. (2007) · Separation and Purification Technology · doi:10.1016/j.seppur.2007.01.008 · Cited by 493 · see citing works
Self-citationda Silva, E. M., de Souza, J. N. S., Rogez, H. L. G., Rees, J., & Larondelle, Y. (2006) · Food Chemistry · doi:10.1016/j.foodchem.2006.02.055 · Cited by 453 · see citing works
Self-citationThe role of antioxidants in photoprotection: A critical review
Chen, L., Hu, J. Y., & Wang, S. Q. (2012) · Journal of the American Academy of Dermatology · doi:10.1016/j.jaad.2012.02.009 · Cited by 402 · see citing works
Zhu, H., Itoh, K., Yamamoto, M. P., Zweier, J. L., & Li, Y. (2005) · FEBS Letters · doi:10.1016/j.febslet.2005.04.058 · Cited by 394 · see citing works
Biomarkers of lipid peroxidation in clinical material
Niki, E. (2014) · Biochimica et Biophysica Acta (BBA) - General Subjects · doi:10.1016/j.bbagen.2013.03.020 · Cited by 297 · see citing works
Active-Oxygen Scavenging Activity of Plant Extracts.
Masaki, H., Sakaki, S., ATSUMI, T., & Sakurai, H. (1995) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.18.162 · Cited by 206 · see citing works
Self-citationTGF-β regulates Nox4, MnSOD and catalase expression, and IL-6 release in airway smooth muscle cells
Michaeloudes, C., Sukkar, M. B., Khorasani, N. M., Bhavsar, P. K., & Chung, K. F. (2010) · American Journal of Physiology-Lung Cellular and Molecular Physiology · doi:10.1152/ajplung.00134.2010 · Cited by 196 · see citing works
da Silva, E. M., Pompeu, D. R., Larondelle, Y., & Rogez, H. L. G. (2006) · Separation and Purification Technology · doi:10.1016/j.seppur.2006.07.012 · Cited by 180 · see citing works
Self-citationFonseca, Y. M., Catini, C. D., Vicentini, F. T. M. C., Nomizo, A., Gerlach, R. F., & Fonseca, M. J. V. (2009) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2009.12.019 · Cited by 175 · see citing works
Self-citationPhotoprotective characteristics of natural antioxidant polyphenols
Stevanato, R., Bertelle, M., & Fabris, S. (2014) · Regulatory Toxicology and Pharmacology · doi:10.1016/j.yrtph.2014.02.014 · Cited by 139 · see citing works
Active ingredients against human epidermal aging
Lorencini, M., Brohem, C. A., Dieamant, G., Zanchin, N. I. T., & Maïbach, H. I. (2014) · Ageing Research Reviews · doi:10.1016/j.arr.2014.03.002 · Cited by 139 · see citing works
Kenny, O., Smyth, T. J., Walsh, D., Kelleher, C. T., Hewage, C. M., & Brunton, N. P. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.03.126 · Cited by 111 · see citing works
Action spectrum for UV-induced lipid peroxidation in cultured human skin fibroblasts
Morlière, P., Moysan, A., & Tirache, I. (1995) · Free Radical Biology and Medicine · doi:10.1016/0891-5849(95)00043-w · Cited by 107 · see citing works
Balboa, E. M., Soto, M. L., Nogueira-Librelotto, D. R., González-López, N., Conde, E., Moure, A., Vinardell, M. P., Mitjans, M., & Domı́nguez, H. (2014) · Industrial Crops and Products · doi:10.1016/j.indcrop.2014.03.041 · Cited by 94 · see citing works
Cai, W., Chen, Y., Xie, L., Zhang, H., & Hou, C. (2013) · European Food Research and Technology · doi:10.1007/s00217-013-2091-x · Cited by 84 · see citing works
Time course of changes in antioxidant enzymes in human skin fibroblasts after UVA irradiation
Shindo, Y., & Hashimoto, T. (1997) · Journal of Dermatological Science · doi:10.1016/s0923-1811(96)00578-6 · Cited by 81 · see citing works
LR-90 prevents methylglyoxal-induced oxidative stress and apoptosis in human endothelial cells
Figarola, J. L., Singhal, J., Rahbar, S., Awasthi, S., & Singhal, S. S. (2014) · APOPTOSIS · doi:10.1007/s10495-014-0974-3 · Cited by 65 · see citing works
Determination of Sun Protection Factor (SPF) number of some aqueous herbal extracts
Malsawmtluangi, C., Nath, D. K., Jamatia, I., Zarzoliana, E., & Pachuau, L. (2013) · Journal of Applied Pharmaceutical Science · doi:10.7324/japs.2013.3925 · Cited by 61 · see citing works
Silva, R. V., Costa, S. C. C., Branco, C. R. C., & Branco, A. (2016) · Industrial Crops and Products · doi:10.1016/j.indcrop.2015.12.077 · Cited by 59 · see citing works
Marquele‐Oliveira, F., Fonseca, Y. M., de Freitas, O., & Fonseca, M. J. V. (2007) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2007.04.026 · Cited by 57 · see citing works
Self-citationQi, H., Dong, X., Zhao, Y.-P., Li, N., Fu, H., Feng, D., Liu, L., & Yu, C. (2015) · Food Chemistry · doi:10.1016/j.foodchem.2015.07.030 · Cited by 56 · see citing works
Exposure of vitamins to UVB and UVA radiation generates singlet oxygen
Knak, A., Regensburger, J., Maisch, T., & Bäumler, W. (2014) · Photochemical & Photobiological Sciences · doi:10.1039/c3pp50413a · Cited by 51 · see citing works
Kim, S., You, D. H., Han, T., & Choi, E.-M. (2014) · Journal of Photochemistry and Photobiology B: Biology · doi:10.1016/j.jphotobiol.2014.10.012 · Cited by 49 · see citing works
Cvetanović, A. D., Švarc‐Gajić, J., Zeković, Z. P., Savic, S. R., Vulić, J., Mašković, P., & Ćetković, G. (2015) · Planta · doi:10.1007/s00425-015-2308-2 · Cited by 48 · see citing works
The intracellular oxidation of 2′,7′-dichlorofluorescin in murine T lymphocytes
van Reyk, D. M., King, N. J. C., Dinauer, M. C., & Hunt, N. H. (2001) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(00)00449-4 · Cited by 45 · see citing works
Importance of UVA photoprotection as shown by genotoxic related endpoints: DNA damage and p53 status
Marrot, L., Belaïdi, J.-P., & Meunier, J. (2005) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2004.07.020 · Cited by 38 · see citing works
Aragão, D. M. D. O., Lima, I. V. D. A., da Silva, J. M., Bellozi, P. M. Q., da Costa, J. D. C., Cardoso, G. M. M., de Souza-Fagundes, E. M., & Scio, E. (2012) · Phytotherapy Research · doi:10.1002/ptr.4811 · Cited by 35 · see citing works
Jaszewska, E., Soin, M., Filipek, A., & Naruszewicz, M. (2013) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2013.07.001 · Cited by 32 · see citing works
Free-radical scavenging activity and antibacterial impact of Greek oregano isolates obtained by SFE
Stamenić, M., Vulić, J., Djilas, S., Mišić, D., Tadić, V., Petrovic, S. D., & Žižović, I. (2014) · Food Chemistry · doi:10.1016/j.foodchem.2014.05.091 · Cited by 32 · see citing works
Gazal, M., Ortmann, C. F., Martins, F. A., STRECK, E. L., Quevedo, J., de Campos, Á. M., Stefanello, F. M., Kaster, M. P., Ghisleni, G., Reginatto, F. H., et al. (2014) · Brain Research Bulletin · doi:10.1016/j.brainresbull.2014.07.007 · Cited by 31 · see citing works
Huang, B., Zhu, L., Liu, S., Li, D., Chen, Y., Ma, B., & Wang, Y. (2013) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2013.02.005 · Cited by 30 · see citing works
da Silva, E. M., da Silva, J. S., Pena, R. D. S., & Rogez, H. L. G. (2010) · Food and Bioproducts Processing · doi:10.1016/j.fbp.2010.03.004 · Cited by 28 · see citing works
Self-citationSinglet-oxygen-derived products from linoleate activate Nrf2 signaling in skin cells
Ogawa, Y., Shichiri, M., Nishio, K., Yoshida, Y., Niki, E., & Hagihara, Y. (2014) · Free Radical Biology and Medicine · doi:10.1016/j.freeradbiomed.2014.12.004 · Cited by 27 · see citing works
Gazal, M., Kaufmann, F. N., Acosta, B. A., Oliveira, P. S., Valente, M. R., Ortmann, C. F., Sturbelle, R. T., Lencina, C. L., Stefanello, F. M., Kaster, M. P., et al. (2015) · Neurochemical Research · doi:10.1007/s11064-015-1610-5 · Cited by 27 · see citing works
Farooqı, A. A., Li, R., Huang, H., Ismail, M., Yuan, S. F., Wang, H., Liu, J., Tang, J., & Chang, H. (2015) · Current Pharmaceutical Biotechnology · doi:10.2174/1389201016666150731111308 · Cited by 15 · see citing works
Arend, D. P., dos Santos, T. C., Cazarolli, L. H., Hort, M. A., Sonaglio, D., dos Santos, A. L. G., Ribeiro‐do‐Valle, R. M., Silva, F. R. M. B., & de Campos, Á. M. (2015) · Revista Brasileira de Farmacognosia · doi:10.1016/j.bjp.2015.05.010 · Cited by 12 · see citing works
Laquièze, L., Lorencini, M., & Granjeiro, J. M. (2015) · Applied In Vitro Toxicology · doi:10.1089/aivt.2015.0008 · Cited by 11 · see citing works
Shairibha, S. M. R., & Rajadurai, M. (2014) · Cited by 7 · see citing works
The protective effect of laver extract against the UVA- and UVB-induced damage in HaCaT cells
Yang, S.-J., Han, T., & Choi, E. (2012) · Toxicology and Environmental Health Sciences · doi:10.1007/s13530-012-0134-5 · Cited by 2 · see citing works
Flagged first, then by citations
3 of the 51 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
UV Filters: Challenges and Prospects
Jesus, A., Sousa, E., Cruz, M., Cidade, H., Lobo, J., & Almeida, I. (2022) · Pharmaceuticals · doi:10.3390/ph15030263 · Cited by 132 · see citing works
Bose, B., Choudhury, H., Tandon, P., & Kumaria, S. (2017) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2017.07.010 · Cited by 117 · see citing works
Espinosa-Leal, C. A., & García‐Lara, S. (2019) · Planta Medica · doi:10.1055/a-0857-6633 · Cited by 60 · see citing works
Bose, B., Tripathy, D., Chatterjee, A., Tandon, P., & Kumaria, S. (2018) · Phytomedicine · doi:10.1016/j.phymed.2018.08.010 · Cited by 50 · see citing works
Wang, L., Ryu, B., Kim, W.-S., Kim, G. H., & Jeon, Y. (2017) · ALGAE · doi:10.4490/algae.2017.32.11.29 · Cited by 44 · see citing works
Alves, G. D. A. D., de Souza, R. O., Rogez, H. L. G., Masaki, H., & Fonseca, M. J. V. (2019) · PLoS ONE · doi:10.1371/journal.pone.0216501 · Cited by 33 · see citing works
Self-citationde Souza, R. O., Alves, G. D. A. D., Forte, A. L. S. A., Rogez, H. L. G., & Fonseca, M. J. V. (2017) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2017.10.033 · Cited by 32 · see citing works
Self-citationSkin penetration and UV ‐damage prevention by nanoberries
Bucci, P., Prieto, M. J., Sanabria, L. N. M., Calienni, M. N., Martinez, L., Rivarola, V. A., Alonso, S. D. V., & Montanari, J. (2017) · Journal of Cosmetic Dermatology · doi:10.1111/jocd.12436 · Cited by 32 · see citing works
de Souza, R. O., Alves, G. D. A. D., Forte, A. L. S. A., Marquele‐Oliveira, F., da Silva, D. F., Rogez, H. L. G., & Fonseca, M. J. V. (2017) · Industrial Crops and Products · doi:10.1016/j.indcrop.2017.07.015 · Cited by 21 · see citing works
Self-citationMartins, R. M., Alves, G. D. A. D., Martins, S. D. S., de Freitas, L. A. P., Rochette, P. J., Moulin, V. J., & Fonseca, M. J. V. (2020) · Rejuvenation Research · doi:10.1089/rej.2019.2219 · Cited by 19 · see citing works
Self-citationXu, X., Sun, G., Liu, J., Zhou, J., Li, J., Sun, Z. R., Li, X., Chen, H., Zhao, D., Jiang, R., et al. (2021) · Wound Repair and Regeneration · doi:10.1111/wrr.12962 · Cited by 8 · see citing works
Rivera-Mondragón, A., Ortiz, O. O., Gupta, M. P., & Caballero‐George, C. (2021) · Planta Medica · doi:10.1055/a-1495-9785 · Cited by 8 · see citing works
A novel research model for evaluating sunscreen protection in the UV-A1
Figueiredo, S. A., de Moraes, D. C., Vilela, F. M. P., de Faria, A. N., dos Santos, M. H., & Fonseca, M. J. V. (2017) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2017.10.031 · Cited by 5 · see citing works
Self-citationAlves, G. D. A. D., da Silva, D. F., Teixeira, T. V., de Souza, R. O., Rogez, H. L. G., & Fonseca, M. J. V. (2019) · Preparative Biochemistry & Biotechnology · doi:10.1080/10826068.2019.1658118 · Cited by 4 · see citing works
Self-citationLalthafamkimi, L., Kumar, A., Wann, S. B., Kumar, D., Bhattacharyya, P., & Kumar, S. (2023) · Industrial Crops and Products · doi:10.1016/j.indcrop.2023.116689 · Cited by 1 · see citing works
Pereira, D. T., Bouzon, Z. L., & Simioni, C. (2019) · Geosul · doi:10.5007/1982-5153.2019v34n73p175
Influência da encapsulação e associação de derivados da avobenzona na performance de filtros solares
Gluzezak, A. J. P. (2021) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.60.2021.tde-29092021-064024
Azevedo, C. B. D. S. (2021) · Digital Library of Theses and Dissertations (Universidade de São Paulo) · doi:10.11606/d.60.2021.tde-22032022-074324
Azevedo, S. C. M., Amorim, B. S., Zauli, R. C., Mendonça, L. D. S., Marinho, L. D. N., Vieira, M. M., Carvalho, K. M., da Silva, G. F., da Silva, W. L., Falcão, L. D. S., et al. (2024) · Processes · doi:10.3390/pr12102237
- Authors
- Alves, G. D. A. D., de Souza, R. O., Rogez, H., Masaki, H., & Fonseca, M. J. V.
- Affiliations
- Tokyo University of Technology (JP)
- Universidade de São Paulo (BR)
- Corresponding author
- Fonseca, Maria José Vieira
- Venue
- Industrial Crops and Products
- Publisher
- Elsevier BV
- Published
- 2016-12
- Volume
- 94
- Pages
- 893-902
- ISSN
- 0926-6690 (ISSN-L)
- OpenAlex
- W2529407431
- Cited by
- 20
- References
- 51
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 4 · 2020: 1 · 2019: 4 · 2018: 1 · 2017: 6
- FWCI
- 4.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Industrial Crops and Products (publishedVersion)
- Funders
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo a Pesquisa do Estado de São Paulo
- Fundo da Amazônia
- Brazilian Genetic Heritage Management Council (010526/2015-3)
- Topics
- Health Sciences › Medicine › Dermatology › Skin Protection and Aging
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Medicinal Plant Research
- Keywords
- ethanolic leaf extract, UVA, keratinocytes, antioxidant capacity, superoxide, singlet oxygen, lipid peroxidation, redox homeostasis, polyphenols, flavonoids, UV filters, cosmetic formulation, oxidative damage
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
