Cited by 138Neuroinflammation and Neurodegeneration MechanismsFlavonoids in Medical ResearchGenomics, phytochemicals, and oxidative stress

Quercetin Inhibits Lipopolysaccharide-Induced Nitric Oxide Production in BV2 Microglial Cells by Suppressing the NF-Κb Pathway and Activating the Nrf2-dependent HO-1 Pathway

Kang, Chang-Hee, Choi, Yung Hyun, Moon, Sung-Kwon, Kim, Wun-Jae, Kim, Gi-Young·2013·International Immunopharmacology

Abstract

Abnormal nitrosative stress-induced neuroinflammation is implicated in the pathogenesis of neurodegenerative diseases. Therefore, it has been thought that nitric oxide (NO) production is a good therapeutic target. In this sense, quercetin is a good chemopreventive component, because it has free radical-scavenging and anti-inflammatory activities. However, explicit mechanisms are not clear in the lipopolysaccharide (LPS)-stimulated BV2 microglial cell line. Here, we found that quercetin significantly suppressed LPS-induced NO production and inducible NO synthase (iNOS) expression. Notably, quercetin inhibited nuclear factor-κB (NF-κB) activation by inhibiting degradation of the inhibitor of kappa Bα (IκBα) in LPS-stimulated BV2 microglial cells corresponding to the inhibitory effect of specific NF-κB inhibitors, namely proteasome inhibitor I (PSI) and MG132. Quercetin caused significant increases in the levels of heme oxgenase-1 (HO-1) mRNA and protein. Notably, treatment with an HO-1 inducer, cobalt protoporphyrin (CoPP), significantly diminished LPS-stimulated NO production. Additionally, quercetin induced the specific DNA-binding activity of nuclear factor-2-erythroid 2-related factor 2 (Nrf2), and siRNA-mediated knockdown of Nrf2 expression reduced the inhibitory effect of quercetin on LPS-stimulated NO production by inhibiting HO-1 expression, indicating that quercetin regulated NO production by inducing Nrf2-mediated HO-1 expression. Therefore, quercetin has the potential to decrease nitrosative stress by suppressing NF-κB activation and inducing Nrf2-mediated HO-1 expression.

Integrity signals

Registry

Crossref · 10.1016/j.intimp.2013.09.009

Cites

38 of 38 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Cites

Flagged first, then by citations

2 of the 40 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Kang, C.-H., Choi, Y. H., Moon, S.-K., Kim, W.-J., & Kim, G.-Y.
Affiliations
  • Jeju National University (KR)
  • Dong-Eui University (KR)
  • Chung-Ang University (KR)
  • Chungbuk National University (KR)
Corresponding author
Kim, Gi-Young
Venue
International Immunopharmacology
Publisher
Elsevier BV
Published
2013-11
Volume
17
Issue
3
Pages
808-813
ISSN
1567-5769 (ISSN-L)
OpenAlex
W4247659709
Cited by
138
References
40
Citations by year
2026: 6 · 2025: 12 · 2024: 10 · 2023: 6 · 2022: 8 · 2021: 11 · 2020: 11 · 2019: 8 · 2018: 11 · 2017: 14 · 2016: 18 · 2015: 15 · 2014: 7
FWCI
7.47
Type
journal article
Language
en
Open access
closed
Locations
  • International Immunopharmacology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Ministry of Education, Science and Technology (2008-0062611)
  • National Research Foundation of Korea
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Health Sciences › Medicine › Pharmacology › Flavonoids in Medical Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics, phytochemicals, and oxidative stress
Keywords
quercetin, nitric oxide production, lipopolysaccharide, BV2 microglial cells, NF-κB signaling, Nrf2, heme oxygenase-1, inducible nitric oxide synthase, IκBα degradation, neuroinflammation, nitrosative stress, cobalt protoporphyrin, neurodegenerative diseases
MeSH terms
Animals, Cell Line, Lipopolysaccharides, Membrane Proteins, Nitric Oxide, Quercetin, NF-kappa B, Microglia, Neuroprotective Agents, NF-E2-Related Factor 2, Mice, Heme Oxygenase-1, Nitric Oxide Synthase Type II

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review