Taming the Wild West of Organoids, Enteroids, and Mini-Guts
Open accessAbstract
The complex nature of an organ, made up of multiple tissue and cells types, poses an incredible challenge to biologists wishing to interrogate homeostasis or disease. Because of this complexity, it often is difficult to ascertain the cause-and-effect relationship of an experimental manipulation in these systems. For example, in the intestine, a perturbation to the epithelium may elicit an immune response; however, it is difficult to determine if this response is a direct result of the epithelial perturbation, or a consequence of a secondary effect, such as translocation of bacteria across the epithelium.
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Lot-to-lot variations of Matrigel compound the difficulty of identifying the signals necessary for organoid structure and function.
“The undefined nature of Matrigel makes it difficult to identify the signals necessary for organoid structure and function; this difficulty is compounded by lot-to-lot variations of Matrigel45–48.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [47]
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Self-citationGastrointestinal Organoids: Understanding the Molecular Basis of the Host–Microbe Interface
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Self-citationLGR4 and LGR5 Function Redundantly During Human Endoderm Differentiation
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Towards organoid culture without Matrigel
Kozlowski, M. T., Crook, C. J., & Ku, H. T. (2021) · Communications Biology · doi:10.1038/s42003-021-02910-8 · Cited by 409 · see citing works
Intestinal organoids: A new paradigm for engineering intestinal epithelium in vitro
Rahmani, S., Breyner, N. M., Su, H.-M., Verdu, E. F., & Didar, T. F. (2019) · Biomaterials · doi:10.1016/j.biomaterials.2018.12.006 · Cited by 98 · see citing works
- Authors
- Spence, J. R.
- Affiliations
- University of Michigan (US)
- Corresponding author
- Spence, Jason R.
- ORCID
- Spence, Jason R.
- Venue
- Cellular and Molecular Gastroenterology and Hepatology
- Publisher
- Elsevier BV
- Published
- 2018
- Volume
- 5
- Issue
- 2
- Pages
- 159-160
- ISSN
- 2352-345X (ISSN-L)
- PMC
- PMC5904044
- OpenAlex
- W2769542972
- Cited by
- 24
- References
- 12
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 3 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 5 · 2018: 2
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cellular and Molecular Gastroenterology and Hepatology (publishedVersion, cc-by-nc-nd)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-nd)
- PubMed Central (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- Intestinal Stem Cell Consortium (U01DK103141)
- National Institute of Allergy and Infectious Diseases Novel Alternative Model Systems for Enteric Diseases consortium (U19AI116482)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- organoids, enteroids, intestinal epithelium, immune response, bacterial translocation, model complexity, intestinal homeostasis, intestinal diseases
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