The DOI matches its Crossref record; 43 of the 43 references it cites were found in a registry or an index.
Purification of novel anti-inflammatory peptides from enzymatic hydrolysate of the edible microalgal Spirulina maxima
Open accessAbstract
Abstract Natural bioactive peptides have been known as ingredients in nutraceuticals and pharmaceuticals. In this study, the potential anti-inflammatory peptides were purified from enzymatic hydrolysate of the edible microalgae Spirulina maxima using gastrointestinal endopeptidases (trypsin, α-chymotrypsin, and pepsin). Purification of the hydrolysate resulted in two peptides with amino acid sequences of LDAVNR (P1, 686 Da) and MMLDF (P2, 655 Da). It was found that peptides P1 and P2 exhibited significant inhibition on the release and production of histamine from antigen-stimulated RBL-2H3 mast cells. Moreover, the suppressive effect of P1 and P2 on production as well as expression of interleukin-8 in histamine-stimulated EA.hy926 endothelial cells was determined. Notably, the production of intracellular reactive oxygen species from mast cells and endothelial cells was decreased in the presence of P1 or P2. Collectively, the peptides P1 and P2 from S. maxima could be used as functional ingredients with potent anti-inflammatory benefits.
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In the citing paper
“Both P1 and P2 exhibited a suppressive effect on IL-8 expression in histamine-stimulated EA.hy926 endothelial cells, and on the production of intracellular reactive oxygen species (ROS) in the mast and endothelial cells [33].”
From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [33]
In the citing paper
“Two novel anti-inflammatory peptides with amino acid sequences of LDAVNR (P1, 686 Da) and MMLDF (P2, 655 Da) were purified from the enzymatic hydrolysate of Spirulina maxima.”
From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [33]
In the citing paper
“Both P1 and P2 exhibited a suppressive effect on IL-8 expression in histamine-stimulated EA.hy926 endothelial cells, and on the production of intracellular reactive oxygen species (ROS) in the mast and endothelial cells [33].”
From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [33]
Citations
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Cited by
Sorted by citations
Algal Proteins: Extraction, Application, and Challenges Concerning Production
Bleakley, S., & Hayes, M. (2017) · Foods · doi:10.3390/foods6050033 · Cited by 1,092
“maxima (Leu-Asp-Ala-Val-Asn-Arg and Met-Met-Leu-Asn-Phe), which were additionally reported to display anti-atherosclerosis bioactivity [226,227].”
The antioxidant, immunomodulatory, and anti-inflammatory activities of Spirulina: An overview
Wu, Q., Liu, L., Miron, A., Klímová, B., Wan, D., & Kuča, K. (2016) · Archives of Toxicology · doi:10.1007/s00204-016-1744-5 · Cited by 655
Immunomodulatory and anticancer protein hydrolysates (peptides) from food proteins: A review
Chalamaiah, M., Yu, W., & Wu, J. (2017) · Food Chemistry · doi:10.1016/j.foodchem.2017.10.087 · Cited by 601
“…tyrosine, tryptophan, cysteine, asparagine, and aspartic acid facilitate the immunomodulatory activities of food protein originated peptides (Chen, Suetsuna, & Yamauchi, 1995; He et al., 2015; Hou, Fan, Li, Xue, & Yu, 2012; Kim, Kim, Hwang, Kang et al., 2013; Lee et al., 2012; Vo et al., 2013).”
BackgroundInfluentialA review of high value-added molecules production by microalgae in light of the classification
Levasseur, W., Perré, P., & Pozzobon, V. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107545 · Cited by 575
Zaky, A. A., Simal‐Gándara, J., Eun, J., Shim, J., & El‐Aty, A. M. A. (2022) · Frontiers in Nutrition · doi:10.3389/fnut.2021.815640 · Cited by 475
Metadata
- Authors
- Vo, T.-S., Ryu, B., & Kim, S.-K.
- Affiliations
- Pukyong National University (KR)
- The University of Queensland (AU)
- Corresponding author
- Kim, Se-Kwon
- Venue
- Journal of Functional Foods
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 5
- Issue
- 3
- Pages
- 1336-1346
- ISSN
- 1756-4646 (ISSN-L)
- OpenAlex
- W2083421143
- Semantic Scholar
- 08ed729b45ce31cef2ca7b019a57bb5d305e4885
- Cited by
- 209
- References
- 43
- Citations by year
- 2026: 16 · 2025: 29 · 2024: 25 · 2023: 22 · 2022: 27 · 2021: 21 · 2020: 13 · 2019: 14 · 2018: 13 · 2017: 7 · 2016: 9 · 2015: 4 · 2014: 7 · 2013: 2
- FWCI
- 3.28
- Type
- journal article
- Language
- en
- Open access
- green
- doaj.org
- Licence
- Locations
- Journal of Functional Foods (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Immunology and Microbiology › Immunology › Mast cells and histamine
- Keywords
- anti-inflammatory peptides, Spirulina maxima, enzymatic hydrolysate, peptide purification, histamine release, mast cells, RBL-2H3 cells, interleukin-8, endothelial cells, reactive oxygen species, microalgae, nutraceuticals
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