Cited by 179Aquaculture disease management and microbiotaMicrobial Community Ecology and PhysiologyAquaculture Nutrition and Growth

Bacterial population in intestines of the Black Tiger Shrimp (Penaeus monodon) under different growth stages

Open access
Rungrassamee, Wanilada, Klanchui, Amornpan, Chaiyapechara, Sage, Maibunkaew, Sawarot, Tangphatsornruang, Sithichoke, Jiravanichpaisal, Pikul, Karoonuthaisiri, Nitsara·2013·PLoS ONE

Abstract

Intestinal bacterial communities in aquaculture have been drawn to attention due to potential benefit to their hosts. To identify core intestinal bacteria in the black tiger shrimp (Penaeus monodon), bacterial populations of disease-free shrimp were characterized from intestines of four developmental stages (15-day-old post larvae (PL15), 1- (J1), 2- (J2), and 3-month-old (J3) juveniles) using pyrosequencing, real-time PCR and denaturing gradient gel electrophoresis (DGGE) approaches. A total of 25,121 pyrosequencing reads (reading length = 442±24 bases) were obtained, which were categorized by barcode for PL15 (7,045 sequences), J1 (3,055 sequences), J2 (13,130 sequences) and J3 (1,890 sequences). Bacteria in the phyla Bacteroides, Firmicutes and Proteobacteria were found in intestines at all four growth stages. There were 88, 14, 27, and 20 bacterial genera associated with the intestinal tract of PL15, J1, J2 and J3, respectively. Pyrosequencing analysis revealed that Proteobacteria (class Gammaproteobacteria) was a dominant bacteria group with a relative abundance of 89% for PL15 and 99% for J1, J2 and J3. Real-time PCR assay also confirmed that Gammaproteobacteria had the highest relative abundance in intestines from all growth stages. Intestinal bacterial communities from the three juvenile stages were more similar to each other than that of the PL shrimp based on PCA analyses of pyrosequencing results and their DGGE profiles. This study provides descriptive bacterial communities associated to the black tiger shrimp intestines during these growth development stages in rearing facilities.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1371/journal.pone.0060802

Cites

76 of 76 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 77 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Rungrassamee, W., Klanchui, A., Chaiyapechara, S., Maibunkaew, S., Tangphatsornruang, S., Jiravanichpaisal, P., & Karoonuthaisiri, N.
Affiliations
National Center for Genetic Engineering and Biotechnology (TH)
Corresponding author
Karoonuthaisiri, Nitsara
Venue
PLoS ONE
Published
2013-04-05
Volume
8
Issue
4
Pages
e60802
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2020616960
Cited by
179
References
77
Citations by year
2026: 4 · 2025: 13 · 2024: 18 · 2023: 17 · 2022: 18 · 2021: 25 · 2020: 19 · 2019: 11 · 2018: 17 · 2017: 13 · 2016: 9 · 2015: 9 · 2014: 4 · 2013: 1
FWCI
4.24
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, other-oa)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • Figshare (submittedVersion, cc-by)
  • Greater South Information System
Funders
  • Thailand Research Fund (TRG5480014)
  • Burapha University
  • National Center for Genetic Engineering and Biotechnology
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
Penaeus monodon, intestinal bacterial community, gut microbiota, growth stages, postlarvae, juveniles, Gammaproteobacteria, Bacteroides, Firmicutes, shrimp aquaculture
MeSH terms
Animals, Bacteria, DNA, Bacterial, Intestines, Larva, Time Factors, Sequence Analysis, DNA, Aquaculture, Principal Component Analysis, Penaeidae

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review