A high aspect ratio microelectrode array for mapping neural activity in vitro
Open accessAbstract
A novel high-aspect-ratio penetrating microelectrode array was designed and fabricated for the purpose of recording neural activity. The array allows two dimensional recording of 64 sites in vitro with high aspect ratio penetrating electrodes. Traditional surface electrode arrays, although easy to fabricate, do not penetrate to the viable tissue such as central hippocampus slices and thus have a lower signal/noise ratio and lower selectivity than a penetrating array. In the unfolded hippocampus preparation, the CA1-CA3 pyramidal cell layer in the whole unfolded rodent hippocampus preparation is encased by the alveus on one side and the Schaffer tract on the other and requires penetrating electrodes for high signal to noise ratio recording. An array of 64 electrode spikes, each with a target height of 200μm and diameter of 20μm, was fabricated in silicon on a transparent glass substrate. The impedance of the individual electrodes was measured to be approximately 1.5MΩ±497kΩ. The signal to noise ratio was measured and found to be 19.4±3dB compared to 3.9±0.8dB S/N for signals obtained with voltage sensitive dye RH414. A mouse unfolded hippocampus preparation was bathed in solution containing 50 micro-molar 4-amino pyridine and a complex two dimensional wave of activity was recorded using the array. These results indicate that this novel penetrating electrode array is able to obtain data superior to that of voltage sensitive dye techniques for broad field two-dimensional neuronal activity recording. When used with the unfolded hippocampus preparation, the combination forms a uniquely capable tool for imaging hippocampal network activity in the entire hippocampus.
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Some neural interface devices have been fabricated with high electrode densities, but their designs have limited their use to specific central nervous system structures such as cortical grey matter or hippocampal slices.
“Some neural interface devices have been fabricated with high electrode densities; however, their designs have limited the use of these arrays to specific central nervous system structures, such as cortical grey matter (6.25 electrodes/mm 2 [12,13]; >150 electrodes/mm 2 [17,18]) or hippocampal slices (<11 electrodes/mm 2 [19]).”
From A new high-density (25 electrodes/mm<sup>2</sup>) penetrating microelectrode array for recording and stimulating sub-millimeter neuroanatomical structures (2013) · cites this paper as [19]
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- Authors
- Kibler, A. B., Jamieson, B. G., & Durand, D. M.
- Affiliations
- Case Western Reserve University (US)
- Goddard Space Flight Center (US)
- Corresponding author
- Durand, Dominique M.
- ORCID
- Durand, Dominique M.
- Venue
- Journal of Neuroscience Methods
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 204
- Issue
- 2
- Pages
- 296-305
- ISSN
- 0165-0270 (ISSN-L)
- PMC
- PMC3273647
- OpenAlex
- W2017397002
- Cited by
- 25
- References
- 22
- Citations by year
- 2026: 1 · 2024: 2 · 2022: 2 · 2021: 2 · 2020: 2 · 2018: 1 · 2016: 2 · 2015: 4 · 2014: 5 · 2013: 3 · 2012: 1
- FWCI
- 1.19
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Neuroscience Methods (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- NIH (R01NS40785)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Life Sciences › Neuroscience › Cognitive Neuroscience › Neural dynamics and brain function
- Physical Sciences › Engineering › Biomedical Engineering › Analog and Mixed-Signal Circuit Design
- Keywords
- high aspect ratio electrodes, silicon micromachining, electrode impedance, signal-to-noise ratio, 4-aminopyridine, hippocampal network activity
- MeSH terms
- Action Potentials, Animals, Brain Mapping, Electric Stimulation, Electrophysiology, Microelectrodes, Microscopy, Electron, Scanning, Nerve Net, Neurons, 4-Aminopyridine, Potassium Channel Blockers, Tissue Culture Techniques, Mice, CA1 Region, Hippocampal, CA3 Region, Hippocampal, Signal-To-Noise Ratio
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