miRNA extracted from extracellular vesicles is a robust biomarker of amyotrophic lateral sclerosis
Open accessAbstract
BACKGROUND AND OBJECTIVES: We examined miRNA biomarkers for ALS extracted from extracellular vesicles in blood samples using a large and diverse patient and control population. Different blood collection and storage protocols by different investigators could impact repeatability of miRNA analysis. We tested the hypotheses that miRNA extracted from extracellular vesicles using immunoaffinity purification techniques are robust and repeatable across investigators, laboratories and in a broad ALS population. METHODS: De-identified patient blood plasma samples obtained from the U.S. National ALS Biorepository were compared with plasma from non-ALS controls. Extracellular vesicles were extracted and isolated using L1CAM immunoaffinity purification. Total RNA was extracted, and miRNA quantified using qPCR following careful quality control measures. Gene fold expressions of eight miRNAs were compared using a Mann-Whitney two-tailed test. RESULTS: One hundred blinded, blood plasma samples were analyzed. Thirty-five men and 15 women with ALS were compared with controls consisting of 30 men and 20 women. None of the ALS patient cohort reported family members with ALS suggesting sporadic ALS. Five of the eight biomarkers previously published were found to significantly discriminate ALS patient samples from control samples. DISCUSSION: The methods used in this study provide a repeatable measure of miRNA biomarkers that statistically differentiate ALS patient samples from control samples. The broad inclusion criteria for both the ALS patient cohort and controls along with the collection of blood samples by different investigators suggest that these methods are robust and represent good candidates for further research and development aimed at clinical application.
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miR-146a-5p is up-regulated while miR-4454 is down-regulated in blood plasma-derived EVs of ALS patients compared to healthy controls.
“Banack et al. confirmed previous studies reporting that miR-146a-5p is up-regulated while miR-4454 is down-regulated in the blood plasma-derived EVs of ALS patients compared to healthy controls [267].”
From Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions (2024) · cites this paper as [267]
Cites
Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., & Speleman, F. (2002) · Genome Biology · doi:10.1186/gb-2002-3-7-research0034 · Cited by 20,038 · see citing works
Hellemans, J., Mortier, G., De Paepe, A., Speleman, F., & Vandesompele, J. (2007) · Genome Biology · doi:10.1186/gb-2007-8-2-r19 · Cited by 4,023 · see citing works
Exosomes are released by cultured cortical neurones
Fauré, J., Lachenal, G., Court, M., Hirrlinger, J., Chatellard-Causse, C., Blot, B., Grange, J., Schoehn, G., Goldberg, Y., Boyer, V., et al. (2006) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2005.12.003 · Cited by 896 · see citing works
Maness, P. F., & Schachner, M. (2006) · Nature Neuroscience · doi:10.1038/nn1827 · Cited by 850 · see citing works
Rathjen, F. G., & Schachner, M. (1984) · The EMBO Journal · doi:10.1002/j.1460-2075.1984.tb01753.x · Cited by 741 · see citing works
Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study
Mestdagh, P., Hartmann, N., Baeriswyl, L., Andreasen, D., Bernard, N., Chen, C., Cheo, D., D'Andrade, P., DeMayo, M., Dennis, L., et al. (2014) · Nature Methods · doi:10.1038/nmeth.3014 · Cited by 619 · see citing works
Lachenal, G. M., Pernet‐Gallay, K., Chivet, M., Hemming, F. J., Belly, A., Bodon, G., Blot, B., Haase, G., Goldberg, Y., & Sadoul, R. (2010) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2010.11.004 · Cited by 595 · see citing works
Modulating inflammatory monocytes with a unique microRNA gene signature ameliorates murine ALS
Butovsky, O., Siddiqui, S., Gabriely, G., Lanser, A. J., Dake, B., Murugaiyan, G., Doykan, C. E., Wu, P. M., Gali, R. R., Iyer, L. K., et al. (2012) · Journal of Clinical Investigation · doi:10.1172/jci62636 · Cited by 481 · see citing works
The regulation and functions of the nuclear RNA exosome complex
Kilchert, C., Wittmann, S., & Vasiljeva, L. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2015.15 · Cited by 444 · see citing works
Increased lipid peroxidation in sera of ALS patients
Simpson, E. P., Henry, Y. K., Henkel-Tigges, J., Smith, R. G., & Appel, S. H. (2004) · Neurology · doi:10.1212/wnl.62.10.1758 · Cited by 328 · see citing works
L1CAM is not associated with extracellular vesicles in human cerebrospinal fluid or plasma
Norman, M., Ter‐Ovanesyan, D., Trieu, W., Lazarovits, R., Kowal, E. J. K., Lee, J.-H., Chen‐Plotkin, A. S., Regev, A., Church, G. M., & Walt, D. R. (2021) · Nature Methods · doi:10.1038/s41592-021-01174-8 · Cited by 255 · see citing works
Time to diagnosis and factors affecting diagnostic delay in amyotrophic lateral sclerosis
Richards, D., Morren, J. A., & Pioro, E. P. (2020) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2020.117054 · Cited by 185 · see citing works
Campos‐Melo, D., Droppelmann, C. A., He, Z., Volkening, K., & Strong, M. J. (2013) · Molecular Brain · doi:10.1186/1756-6606-6-26 · Cited by 160 · see citing works
Electrical impedance myography as a biomarker to assess ALS progression
Rutkove, S. B., Caress, J. B., Cartwright, M. S., Burns, T. M., Warder, J., David, W. S., Goyal, N., Maragakis, N. J., Clawson, L., Benatar, M. G., et al. (2012) · Amyotrophic Lateral Sclerosis · doi:10.3109/17482968.2012.688837 · Cited by 143 · see citing works
Saucier, D., Wajnberg, G., Roy, J. W., Beauregard, A., Chacko, S. M., Crapoulet, N., Fournier, S., Ghosh, A., Lewis, S. M., Marrero, A., et al. (2018) · Brain Research · doi:10.1016/j.brainres.2018.12.016 · Cited by 134 · see citing works
Liguori, M., Nuzziello, N., Introna, A., Consiglio, A., Licciulli, F., D’Errico, E., Scarafino, A., Distaso, E., & Simone, I. L. (2018) · Frontiers in Molecular Neuroscience · doi:10.3389/fnmol.2018.00288 · Cited by 131 · see citing works
Benatar, M. G., & Wuu, J. (2012) · Neurology · doi:10.1212/wnl.0b013e31826e9b1d · Cited by 121 · see citing works
Bustin, S., & Nolan, T. (2017) · European Journal of Clinical Investigation · doi:10.1111/eci.12801 · Cited by 113 · see citing works
Shepheard, S. R., Wuu, J., Cardoso, M., Wiklendt, L., Dinning, P. G., Chataway, T., Schultz, D. W., Benatar, M. G., & Rogers, M. (2017) · Neurology · doi:10.1212/wnl.0000000000003741 · Cited by 111 · see citing works
Banack, S. A., Dunlop, R. A., & Cox, P. A. (2020) · Open Biology · doi:10.1098/rsob.200116 · Cited by 103 · see citing works
Self-citationAmyotrophic lateral sclerosis is a multifactorial disease
Eisen, A. (1995) · Muscle & Nerve · doi:10.1002/mus.880180711 · Cited by 102 · see citing works
Presymptomatic spinal cord pathology in c9orf72 mutation carriers: A longitudinal neuroimaging study
Querin, G., Bede, P., Mendili, M. M. E., Li, M., Pélégrini‐Issac, M., Rinaldi, D., Catala, M., Saracino, D., Salachas, F., Camuzat, A., et al. (2019) · Annals of Neurology · doi:10.1002/ana.25520 · Cited by 95 · see citing works
Locked nucleic acids in PCR primers increase sensitivity and performance
Ballantyne, K. N., van Oorschot, R. A. H., & Mitchell, R. J. (2008) · Genomics · doi:10.1016/j.ygeno.2007.10.016 · Cited by 87 · see citing works
Xu, Q., Zhao, Y., Zhou, X., Luan, J. Y., Cui, Y., & Han, J. (2018) · Intractable & Rare Diseases Research · doi:10.5582/irdr.2017.01091 · Cited by 83 · see citing works
He, S., Huang, L., Shao, C., Nie, T., Xia, L., Cui, B., Lu, F., Zhu, L., Chen, B., & Yang, Q. (2021) · Translational Neurodegeneration · doi:10.1186/s40035-021-00249-y · Cited by 80 · see citing works
Mehta, P., Raymond, J. S., Punjani, R., Han, M., Larson, T. C., III, Kaye, W., Nelson, L. M., Topol, B., Muravov, O. I., Genson, C., et al. (2022) · Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration · doi:10.1080/21678421.2022.2059380 · Cited by 78 · see citing works
Self-citationpNfH is a promising biomarker for ALS
Ganesalingam, J., An, J., Bowser, R. P., Andersen, P. M., & Shaw, C. E. (2012) · Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration · doi:10.3109/21678421.2012.729596 · Cited by 77 · see citing works
Newell, M. E., Adhikari, S., & Halden, R. U. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.152504 · Cited by 75 · see citing works
Spinal cord markers in ALS: Diagnostic and biomarker considerations
Bede, P., Bokde, A. L. W., Byrne, S., Elamin, M., Fagan, A. J., & Hardiman, O. M. (2012) · Amyotrophic Lateral Sclerosis · doi:10.3109/17482968.2011.649760 · Cited by 71 · see citing works
Circulating RNAs as Potential Biomarkers in Amyotrophic Lateral Sclerosis
Ravnik‐Glavač, M., & Glavač, D. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21051714 · Cited by 68 · see citing works
Dobrowolny, G., Martone, J., Lepore, E., Casola, I., Petrucci, A., Inghilleri, M., Morlando, M., Colantoni, A., Scicchitano, B. M., Calvo, A., et al. (2021) · Cell Death Discovery · doi:10.1038/s41420-020-00397-6 · Cited by 65 · see citing works
RNA metabolism in ALS: When normal processes become pathological
Droppelmann, C. A., Campos‐Melo, D., Ishtiaq, M., Volkening, K., & Strong, M. J. (2014) · Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration · doi:10.3109/21678421.2014.881377 · Cited by 61 · see citing works
Identification of a potential non-coding RNA biomarker signature for amyotrophic lateral sclerosis
Joilin, G., Gray, E., Thompson, A. G., Bobeva, Y., Talbot, K., Weishaupt, J. H., Ludolph, A. C., Malaspina, A., Leigh, P. N., Newbury, S. F., et al. (2020) · Brain Communications · doi:10.1093/braincomms/fcaa053 · Cited by 59 · see citing works
Prevalence of Amyotrophic Lateral Sclerosis — United States, 2010–2011
(2015) · American Journal of Public Health · doi:10.2105/ajph.2015.302747 · Cited by 59 · see citing works
Elevated plasma miR-133b and miR-221-3p as biomarkers for early Parkinson’s disease
Chen, Q., Deng, N., Lu, K., Liao, Q., Long, X., Gou, D., Bi, F., & Zhou, J. (2021) · Scientific Reports · doi:10.1038/s41598-021-94734-z · Cited by 57 · see citing works
Falzone, Y., Domi, T., Mandelli, A., Pozzi, L., Schito, P., Russo, T., Barbieri, A., Fazio, R., Volonté, M. A., Magnani, G., et al. (2022) · European Journal of Neurology · doi:10.1111/ene.15321 · Cited by 56 · see citing works
The presymptomatic phase of amyotrophic lateral sclerosis: are we merely scratching the surface?
Chipika, R. H., Siah, W. F., McKenna, M. C., Shing, S. L. H., Hardiman, O. M., & Bede, P. (2020) · Journal of Neurology · doi:10.1007/s00415-020-10289-5 · Cited by 53 · see citing works
Cortical hyperexcitability: Diagnostic and pathogenic biomarker of ALS
Vucic, S., Pavey, N., Haidar, M., Turner, B. J., & Kiernan, M. C. (2021) · Neuroscience Letters · doi:10.1016/j.neulet.2021.136039 · Cited by 52 · see citing works
Cerebrospinal fluid biomarkers of disease activity and progression in amyotrophic lateral sclerosis
Dreger, M., Steinbach, R., Otto, M., Turner, M. R., & Großkreutz, J. (2022) · Journal of Neurology Neurosurgery & Psychiatry · doi:10.1136/jnnp-2021-327503 · Cited by 49 · see citing works
The Biogenesis of miRNAs and Their Role in the Development of Amyotrophic Lateral Sclerosis
Liu, J., Zhou, F., Guan, Y., Meng, F., Zhao, Z., Su, Q., Bao, W., Wang, X., Zhao, J., Huo, Z., et al. (2022) · Cells · doi:10.3390/cells11030572 · Cited by 49 · see citing works
Phenotypic variability and its pathological basis in amyotrophic lateral sclerosis
Takeda, T., Kitagawa, K., & Arai, K. (2019) · Neuropathology · doi:10.1111/neup.12606 · Cited by 46 · see citing works
RNA Deregulation in Amyotrophic Lateral Sclerosis: The Noncoding Perspective
Laneve, P., Tollis, P., & Caffarelli, E. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms221910285 · Cited by 32 · see citing works
Pansarasa, O., Garofalo, M., Scarian, E., Dragoni, F., Garau, J., Gerlando, R. D., Diamanti, L., Bordoni, M., & Gagliardi, S. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23052580 · Cited by 32 · see citing works
VAPB ER-Aggregates, A Possible New Biomarker in ALS Pathology
Cadoni, M. P. L., Biggio, M. L., Arru, G., Secchi, G., Orrù, N., Clemente, M. G., Sechi, G., Yamoah, A., Tripathi, P., Orrù, S., et al. (2020) · Cells · doi:10.3390/cells9010164 · Cited by 26 · see citing works
C‐reactive protein levels in patients with amyotrophic lateral sclerosis: A systematic review
Kharel, S., Ojha, R., Preethish‐Kumar, V., & Bhagat, R. (2022) · Brain and Behavior · doi:10.1002/brb3.2532 · Cited by 26 · see citing works
Varma-Doyle, A. V., Lukiw, W. J., Zhao, Y., Lovera, J. F., & Devier, D. J. (2020) · Journal of the Neurological Sciences · doi:10.1016/j.jns.2020.117202 · Cited by 25 · see citing works
Exosome microRNAs in Amyotrophic Lateral Sclerosis: A Pilot Study
Pregnolato, F., Cova, L., Doretti, A., Bardelli, D., Silani, V., & Bossolasco, P. (2021) · Biomolecules · doi:10.3390/biom11081220 · Cited by 18 · see citing works
Dunlop, R. A., Banack, S. A., & Cox, P. A. (2021) · Biology Methods and Protocols · doi:10.1093/biomethods/bpab015 · Cited by 16 · see citing works
Self-citationImaging Net Retrograde Axonal Transport In Vivo: A Physiological Biomarker
Lee, P., Kennedy, Z., Wang, Y., Lu, Y., Cefaliello, C., Uyan, Ö., Song, C., Godinho, B. M. D. C., Xu, Z., Rusckowski, M., et al. (2022) · Annals of Neurology · doi:10.1002/ana.26329 · Cited by 16 · see citing works
RNA as a source of biomarkers for amyotrophic lateral sclerosis
Kiaei, L., & Kiaei, M. (2021) · Metabolic Brain Disease · doi:10.1007/s11011-021-00738-z · Cited by 4 · see citing works
Flagged first, then by citations
2 of the 52 references couldn’t be matched to a known paper.
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Bravo-Miana, R. D. C., Arizaga-Echebarria, J. K., & Otaegui, D. (2024) · Translational Neurodegeneration · doi:10.1186/s40035-024-00418-9 · Cited by 62 · see citing works
Malaguarnera, M., & Cabrera‐Pastor, A. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms251810068 · Cited by 49 · see citing works
Zanirati, G., dos Santos, P. G., Alcará, A. M., Bruzzo, F. T. K., Ghilardi, I. M., Wietholter, V., Xavier, F. A. C., Gonçalves, J. I. B., Marinowic, D. R., Shetty, A. K., et al. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25137371 · Cited by 48 · see citing works
Extracellular Vesicles in Amyotrophic Lateral Sclerosis
McCluskey, G., Morrison, K. E., Donaghy, C. G., René, F., Duddy, W. J., & Duguez, S. (2022) · Life · doi:10.3390/life13010121 · Cited by 41 · see citing works
Extracellular Vesicles as Potential Biomarkers in Amyotrophic Lateral Sclerosis
Barbo, M., & Ravnik‐Glavač, M. (2023) · Genes · doi:10.3390/genes14020325 · Cited by 38 · see citing works
Marriott, H., Kabiljo, R., Hunt, G. P., Khleifat, A. A., Jones, A., Troakes, C., Pfaff, A. L., Quinn, J. P., Kõks, S., Dobson, R., et al. (2023) · Acta Neuropathologica Communications · doi:10.1186/s40478-023-01686-8 · Cited by 32 · see citing works
A microRNA diagnostic biomarker for amyotrophic lateral sclerosis
Banack, S. A., Dunlop, R. A., Mehta, P., Mitsumoto, H., Wood, S. P., Han, M., & Cox, P. A. (2024) · Brain Communications · doi:10.1093/braincomms/fcae268 · Cited by 34 · see citing works
Self-citationCNS cell-derived exosome signatures as blood-based biomarkers of neurodegenerative diseases
Park, C., Weerakkody, J. S., Schneider, R., Miao, S., & Pitt, D. (2024) · Frontiers in Neuroscience · doi:10.3389/fnins.2024.1426700 · Cited by 31 · see citing works
Shu, F., Hu, Y., Sarsaiya, S., Jin, L., Yang, X., Liu, F., Zhu, J., Chen, G., & Chen, J. (2025) · Discover Nano · doi:10.1186/s11671-025-04399-0 · Cited by 30 · see citing works
Dunlop, R. A., Banack, S. A., & Cox, P. A. (2023) · RNA Biology · doi:10.1080/15476286.2023.2198805 · Cited by 27 · see citing works
Self-citationRecent Advances in Extracellular Vesicles in Amyotrophic Lateral Sclerosis and Emergent Perspectives
Afonso, G. J. M., Cavaleiro, C., Valero, J., Mota, S. I., & Ferreiro, E. B. (2023) · Cells · doi:10.3390/cells12131763 · Cited by 26 · see citing works
Extracellular vesicles as nanotheranostic platforms for targeted neurological disorder interventions
Choi, H. K., Chen, M., Goldston, L. L., & Lee, K.-B. (2024) · Nano Convergence · doi:10.1186/s40580-024-00426-5 · Cited by 26 · see citing works
Exosomes for neurodegenerative diseases: diagnosis and targeted therapy
Tao, H., & Gao, B. (2024) · Journal of Neurology · doi:10.1007/s00415-024-12329-w · Cited by 26 · see citing works
Challenges associated with using extracellular vesicles as biomarkers in neurodegenerative disease
Couch, Y. (2023) · Expert Review of Molecular Diagnostics · doi:10.1080/14737159.2023.2277373 · Cited by 21 · see citing works
Bagyinszky, E., Hulme, J., & An, S. S. A. (2023) · Cells · doi:10.3390/cells12151948 · Cited by 20 · see citing works
MicroRNA-23a-3p Is Upregulated in Plasma Exosomes of Bulbar-onset ALS Patients and Targets ERBB4
Liu, Y., Ding, M., Pan, S., Zhou, R., Yao, J., Fu, R., Yu, H., & Lu, Z. (2023) · Neuroscience · doi:10.1016/j.neuroscience.2023.05.030 · Cited by 18 · see citing works
Extracellular vesicles in TDP-43 proteinopathies: pathogenesis and biomarker potential
Dellar, E. R., Nikel, L., Fowler, S. L., Vahsen, B. F., Dafinca, R., Feneberg, E., Talbot, K., Turner, M. R., & Thompson, A. G. (2025) · Molecular Neurodegeneration · doi:10.1186/s13024-025-00859-4 · Cited by 17 · see citing works
Gama, H. C., Amorós, M. A., de Araújo, M. A., Sha, C. M., Vieira, M. P. S., Torres, R. G. D., Souza, G. F., Junkes, J. A., Dokholyan, N. V., Gitaí, D. L. G., et al. (2024) · Non-coding RNA Research · doi:10.1016/j.ncrna.2024.02.006 · Cited by 17 · see citing works
miRNA profiling as a complementary diagnostic tool for amyotrophic lateral sclerosis
Cheng, J., Ho, W.-K., Wu, B., Liu, H., & Lin, W. (2023) · Scientific Reports · doi:10.1038/s41598-023-40879-y · Cited by 16 · see citing works
Deng, Z., Chen, H., Chen, J., Du, Z., Zhou, W., & Yuan, Z. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2025.167960 · Cited by 13 · see citing works
Liquid biopsy in neurodegenerative disease: A minimally invasive approach for early diagnosis
Udayakumar, S., Girigoswami, A., & Girigoswami, K. (2025) · Clinica Chimica Acta · doi:10.1016/j.cca.2025.120517 · Cited by 12 · see citing works
Romanò, S., Nele, V., Campani, V., Rosa, G. D., & Cinti, S. (2024) · Analytica Chimica Acta · doi:10.1016/j.aca.2024.342473 · Cited by 12 · see citing works
Wiersema, A. F., Rennenberg, A., Smith, G., Varderidou‐Minasian, S., & Pasterkamp, R. J. (2024) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-024-05522-7 · Cited by 13 · see citing works
Zaman, A., Drake, S., & Fournier, A. E. (2026) · Journal of Neurochemistry · doi:10.1111/jnc.70323 · Cited by 13 · see citing works
Extracellular microRNAs associated with psychiatric symptoms in the Normative Aging Study
Qiu, X., Yazdi, M. D., Wang, C., Kosheleva, A., Wu, H., Vokonas, P., Spiro, A., Laurent, L. C., DeHoff, P., Kubzansky, L. D., et al. (2024) · Journal of Psychiatric Research · doi:10.1016/j.jpsychires.2024.08.017 · Cited by 9 · see citing works
Zhu, Y., Fang, X., Zhang, S., Liao, Y., Lin, H., Chen, P., Yang, M., Huang, J. J., & Wang, X. (2026) · International Journal of Nanomedicine · doi:10.2147/ijn.s589123 · Cited by 11 · see citing works
Therapeutic and diagnostic potential of extracellular vesicles in amyotrophic lateral sclerosis
Ellison, T. J., Stice, S. L., & Yao, Y. (2022) · Extracellular Vesicle · doi:10.1016/j.vesic.2022.100019 · Cited by 8 · see citing works
Liu, S., Feng, A., & Li, Z. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-05010-4 · Cited by 8 · see citing works
Lauria, G., Curcio, R., & Tucci, P. (2023) · Biomolecules · doi:10.3390/biom14010047 · Cited by 8 · see citing works
Rawat, A., Khurana, S., Verma, S., Dhawan, U., Gourie‐Devi, M., Ganguly, N. K., & Taneja, V. (2025) · Scientific Reports · doi:10.1038/s41598-025-04322-8 · Cited by 9 · see citing works
Brusati, A., Ratti, A., Pensato, V., Peverelli, S., Gentilini, D., Bella, E. D., Sorce, M. N., Meneri, M., Gagliardi, D., Corti, S. P., et al. (2022) · Frontiers in Genetics · doi:10.3389/fgene.2022.1055313 · Cited by 6 · see citing works
Kajitani, G. S., Xavier, G., Villena-Rueda, B. E., Karia, B. T. R., & Santoro, M. L. (2024) · Current topics in membranes · doi:10.1016/bs.ctm.2024.06.002 · Cited by 4 · see citing works
de la Monte, S. M., Yang, Y., Prabhu, A., & Tong, M. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26094190 · Cited by 4 · see citing works
Lockard, G., Gordon, J., Schimmel, S. J., Sayed, B. E., Monsour, M., Garbuzova-Davis, S. N., & Borlongan, C. V. (2023) · Neuroprotection/Neuroprotection (Chichester, England. Print) · doi:10.1002/nep3.26 · Cited by 4 · see citing works
Ueno, Y., Morishima, Y., Hata, T., Shindo, A., Murata, H., Saito, T., Nakamura, Y., & Shindo, K. (2024) · Neurobiology of Disease · doi:10.1016/j.nbd.2024.106639 · Cited by 4 · see citing works
Insights into the Versatile Role of Extracellular Vesicles in the Treatment of CNS Disorders
Jana, K., Ghosh, S., Parua, P., Debnath, B., Halder, J., Sahoo, R. K., Rai, V. K., Pradhan, D., Dash, P., Das, C., et al. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-05283-9 · Cited by 3 · see citing works
Bolsinger, M. M., Vivek, N., Singh, J., Challa, A., Zhu, A., Rothell, T., Wang, S., Zhang, T., Zhu, S., Robbins, N., et al. (2026) · Journal of Translational Medicine · doi:10.1186/s12967-026-08562-8 · Cited by 3 · see citing works
miRNA Biomarkers Diagnose Amyotrophic Lateral Sclerosis in Circulating Blood
Dunlop, R. A., Cox, P. A., Mehta, P., Stommel, E. W., & Banack, S. A. (2025) · Molecular Neurobiology · doi:10.1007/s12035-025-05579-w · Cited by 5 · see citing works
Self-citationMarriott, H., Kabiljo, R., Hunt, G. P., Khleifat, A. A., Jones, A., Troakes, C., Pfaff, A. L., Quinn, J. P., Kõks, S., Dobson, R., et al. (2023) · medRxiv · doi:10.1101/2023.04.21.23288942 · Cited by 3 · see citing works
Hou, X., Jiang, J., & Deng, M. (2025) · Journal of Neurology · doi:10.1007/s00415-025-13028-w · Cited by 2 · see citing works
IsomiR utility in amyotrophic lateral sclerosis prognostication
Cohen, Y., Sinai, I., Magen, I., Danino, Y. M., Wuu, J., Malaspina, A., Benatar, M. G., & Hornstein, E. (2025) · Med · doi:10.1016/j.medj.2025.100928 · Cited by 1 · see citing works
Takemoto, Y., Ito, D., Komori, S., Kishimoto, Y., Yamada, S., Hashizume, A., Katsuno, M., & Nakatochi, M. (2024) · BMC Bioinformatics · doi:10.1186/s12859-024-05840-4 · Cited by 1 · see citing works
(2023) · Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration · doi:10.1080/21678421.2023.2260196
Santana, M. M., Silva, P., Pinto, M. M., Gaspar, L. S., Nobre, R. J., Duarte, S., Marques, T. M., Gama‐Carvalho, M., Januário, C., Cunha, I., et al. (2024) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2024.01.04.574044
Blood Test for ALS Looks Promising, Study Finds
Burling, S. (2024) · Neurology Today · doi:10.1097/01.nt.0001081276.84970.18
Early Detection of Amyotrophic Lateral Sclerosis with Time Collection Evaluation
Mehar, P. T. K., Agarwal, T., Suneetha, K., & Ahmad, N. (2024) · Lecture notes in electrical engineering · doi:10.1007/978-981-97-8043-3_32
Exosomes and extracellular vesicles for neurodegenerative diseases
Dutta, S., & Hornung, S. (2024) · Elsevier eBooks · doi:10.1016/b978-0-443-28822-7.00008-8
Serum small non-coding RNA define molecular subtypes in amyotrophic lateral sclerosis
Baindoor, S., Gibriel, H. A. Y., Kool, L., Su, J., Demaegd, K. C., Venø, M. T., van den Berg, L. H., Kjems, J., van Es, M. A., & Prehn, J. H. M. (2025) · Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration · doi:10.1080/21678421.2025.2574690
(2025) · Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration · doi:10.1080/21678421.2025.2564552
Takemoto, Y., Ito, D., Komori, S., Kishimoto, Y., Yamada, S., Hashizume, A., Katsuno, M., & Nakatochi, M. (2024) · Figshare · doi:10.6084/m9.figshare.26736783
Barry-Carroll, L., Varilh, M., Marchaland, F., Chen, C. T., Sadeyen, A.-L., Dupuy, J., McDade, K., Millar, T., Bazinet, R. P., Layé, S., et al. (2026) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.64898/2026.01.22.700328
Hosseinzadeh, F., Kalhor, H., Fath‐Bayati, L., Kheirkhah, A. H., Jalise, S. Z., Yang, P., & Sheykhhasan, M. (2026) · Molecular and translational medicine · doi:10.1007/978-3-032-23810-8_9
Extracellular Vesicle-Derived microRNAs as Diagnostic Biomarkers Across Neurodegenerative Diseases
Tevlek, A. (2026) · IntechOpen eBooks · doi:10.5772/intechopen.1017741
The diagnostic biomarker problem in amyotrophic lateral sclerosis
Benatar, M. G., Wuu, J., Turner, M. R., & Macklin, E. A. (2026) · Brain · doi:10.1093/brain/awag313
- Authors
- Banack, S. A., Dunlop, R. A., Stommel, E. W., Mehta, P., & Cox, P. A.
- Affiliations
- Dartmouth–Hitchcock Medical Center (US)
- Centers for Disease Control and Prevention (US)
- Corresponding author
- Cox, Paul Alan
- Venue
- Journal of the Neurological Sciences
- Publisher
- Elsevier BV
- Published
- 2022-11
- Volume
- 442
- Pages
- 120396
- ISSN
- 0022-510X (ISSN-L)
- OpenAlex
- W4293522954
- Cited by
- 54
- References
- 52
- Citations by year
- 2026: 7 · 2025: 13 · 2024: 18 · 2023: 12 · 2022: 4
- FWCI
- 6.34
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of the Neurological Sciences (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- Centers for Disease Control and Prevention
- Topics
- Health Sciences › Medicine › Neurology › Amyotrophic Lateral Sclerosis Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Keywords
- amyotrophic lateral sclerosis, microRNA biomarkers, extracellular vesicles, blood plasma, biomarker reproducibility, sporadic ALS, plasma microRNA expression
- MeSH terms
- Extracellular Vesicles, Amyotrophic Lateral Sclerosis, Female, Humans, Male, Biomarkers, MicroRNAs, Neural Cell Adhesion Molecule L1
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