Eugenol suppressed the expression of lipopolysaccharide-induced proinflammatory mediators in human macrophages
Abstract
Eugenol is commonly used as an analgesic agent during acute pulpitis and is a major component of root canal sealers. Despite the frequent applications of eugenol in the practice of dentistry, little is known about the role of eugenol under the status of inflammation. This study was aimed to investigate the influence of eugenol on human macrophages (U937) under the stimulation of lipopolysaccharide (LPS). Eugenol was shown to block the release of the bone resorbing mediators, including interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and prostaglandin E2 from LPS-stimulated macrophages. In contrast, eugenol alone did not alter the expression levels of these proinflammatory mediators in macrophages. Consistent with downregulation of bone-resorbing mediators, eugenol suppressed the messenger RNA expression of LPS-induced IL-1beta, TNF-alpha, and cyclooxygenase-2 in macrophages. The results suggest a potential anti-inflammatory effect of eugenol in the acute inflamed pulps and apical periodontitis.
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Eugenol blocks release of IL-1β, TNF-α, and PGE2 in LPS-stimulated human U937 macrophages.
“Other authors also found that eugenol on human macrophages (U937) under the stimulation of LPS blocked the release of IL-1β, TNF-α and PGE2 [119].”
From Antioxidant and Anti-Inflammatory Activities of Essential Oils: A Short Review (2010) · cites this paper as [119]
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- Authors
- Lee, Y.-Y., Hung, S.-L., Pai, S.-F., Lee, Y.-H., & Yang, S.-F.
- Affiliations
- National Yang Ming University (TW)
- Taipei Veterans General Hospital (TW)
- Corresponding author
- Yang, Shue-Fen
- Venue
- Journal of Endodontics
- Publisher
- Elsevier BV
- Published
- 2007-06
- Volume
- 33
- Issue
- 6
- Pages
- 698-702
- ISSN
- 0099-2399 (ISSN-L)
- OpenAlex
- W2089438372
- Cited by
- 87
- References
- 39
- Citations by year
- 2026: 1 · 2025: 4 · 2024: 4 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 5 · 2019: 1 · 2018: 4 · 2017: 4 · 2016: 2 · 2014: 9 · 2013: 7 · 2012: 6
- FWCI
- 6.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Endodontics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Dentistry › Oral Surgery › Endodontics and Root Canal Treatments
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
- Keywords
- eugenol, lipopolysaccharide, macrophages, U937 cells, pro-inflammatory mediators, anti-inflammatory activity, IL-1β, TNF-α, prostaglandin E2, cyclooxygenase-2, bone resorption, apical periodontitis, pulpitis, endodontic sealers
- MeSH terms
- Anti-Inflammatory Agents, Eugenol, Humans, Lipopolysaccharides, Macrophages, Tumor Necrosis Factor-alpha, Dinoprostone, Down-Regulation, Inflammation Mediators, U937 Cells, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors, Interleukin-1beta
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