Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin, a blue-colored biliprotein of microalga Spirulina
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C-phycocyanin, the major protein of cyanobacteria Spirulina, possesses significant antioxidant, anti-cancer, anti-inflammatory and immunomodulatory effects, ascribed to covalently attached linear tetrapyrrole chromophore phycocyanobilin. There are no literature data about structure and biological activities of released peptides with bound chromophore in C-phycocyanin digest. This study aims to identify chromopeptides obtained after pepsin digestion of C-phycocyanin and to examine their bioactivities. C-phycocyanin is rapidly digested by pepsin in simulated gastric fluid. The structure of released chromopeptides was analyzed by high resolution tandem mass spectrometry and peptides varying in size from 2 to 13 amino acid residues were identified in both subunits of C-phycocyanin. Following separation by HPLC, chromopeptides were analyzed for potential bioactivities. It was shown that all five chromopeptide fractions have significant antioxidant and metal-chelating activities and show cytotoxic effect on human cervical adenocarcinoma and epithelial colonic cancer cell lines. In addition, chromopeptides protect human erythrocytes from free radical-induced hemolysis in antioxidative capacity-dependant manner. There was a positive correlation between antioxidative potency and other biological activities of chromopeptides. Digestion by pepsin releases biologically active chromopeptides from C-phycocyanin whose activity is mostly related to the antioxidative potency provided by chromophore.
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Marine Origin Bioactive Peptides: Novel Advances in the Therapeutic Potential
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Role of food protein-derived peptides in the treatment of colon cancer
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Purificación y caracterización de la ficocianobilina
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Analytical Protocols in the Measurement of Pigments’ Bioavailability
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Evaluation of Phycocyanin Promoter Function in Bacteria by Investigating the Expression of HBsAg
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Spirulina: Properties, Benefits and Health-Nutrition Relationship
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Safety evaluation of blue galdieria extract as a food additive
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- Authors
- Minic, S. L., Stanic-Vucinic, D., Mihailovic, J., Krstic, M., Nikolic, M. R., & Cirkovic Velickovic, T.
- Affiliations
- University of Belgrade (RS)
- Venue
- Journal of Proteomics
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 147
- Pages
- 132-139
- ISSN
- 1874-3919 (ISSN-L)
- OpenAlex
- W2342272883
- Cited by
- 83
- References
- 58
- Citations by year
- 2026: 16 · 2025: 8 · 2024: 9 · 2023: 8 · 2022: 10 · 2021: 11 · 2020: 6 · 2019: 4 · 2018: 8 · 2017: 2 · 2016: 1
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- green
- cherry.chem.bg.ac.rs
- Licence
- cc-by-nc-nd
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- Locations
- Journal of Proteomics (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion)
- Cherry (Univesrity of Belgrade, Faculty of Chemistry) (submittedVersion)
- Cherry (Univesrity of Belgrade, Faculty of Chemistry) (submittedVersion, cc-by-nc-nd)
- Funders
- Seventh Framework Programme (256716)
- Ministarstvo Prosvete, Nauke i Tehnološkog Razvoja (172024)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Hydrolysis and Bioactive Peptides
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Keywords
- C-phycocyanin, pepsin digestion, chromopeptides, phycocyanobilin, antioxidant activity, metal chelating activity, cytotoxicity, colon cancer cells, erythrocyte hemolysis, free radical protection, Spirulina, cyanobacteria, simulated gastric fluid
- MeSH terms
- Antioxidants, Cells, Cultured, Uterine Cervical Neoplasms, Colonic Neoplasms, Color, Erythrocytes, Female, Hemolysis, Humans, Pepsin A, Peptides, Phycocyanin, Cell Line, Tumor, Spirulina
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