Cited by 61Neurological Disease Mechanisms and TreatmentsNeurological Disorders and TreatmentsHeme Oxygenase-1 and Carbon Monoxide

Phycocyanobilin promotes PC12 cell survival and modulates immune and inflammatory genes and oxidative stress markers in acute cerebral hypoperfusion in rats

Marín-Prida, Javier, Pavón-Fuentes, Nancy, Llópiz-Arzuaga, Alexey, Fernández-Massó, Julio R., Delgado-Roche, Liván, Mendoza-Marí, Yssel, Santana, Seydi Pedroso, Cruz-Ramírez, Alieski +8 more·2013·Toxicology and Applied Pharmacology

Abstract

Since the inflammatory response and oxidative stress are involved in the stroke cascade, we evaluated here the effects of Phycocyanobilin (PCB, the C-Phycocyanin linked tetrapyrrole) on PC12 cell survival, the gene expression and the oxidative status of hypoperfused rat brain. After the permanent bilateral common carotid arteries occlusion (BCCAo), the animals were treated with saline or PCB, taking samples 24h post-surgery. Global gene expression was analyzed with GeneChip Rat Gene ST 1.1 from Affymetrix; the expression of particular genes was assessed by the Fast SYBR Green RT-PCR Master Mix and Bioplex methods; and redox markers (MDA, PP, CAT, SOD) were evaluated spectrophotometrically. The PCB treatment prevented the H2O2 and glutamate induced PC12 cell injury assessed by the MTT assay, and modulated 190 genes (93 up- and 97 down-regulated) associated to several immunological and inflammatory processes in BCCAo rats. Furthermore, PCB positively modulated 19 genes mostly related to a detrimental pro-inflammatory environment and counteracted the oxidative imbalance in the treated BCCAo animals. Our results support the view of an effective influence of PCB on major inflammatory mediators in acute cerebral hypoperfusion. These results suggest that PCB has a potential to be a treatment for ischemic stroke for which further studies are needed.

Integrity signals

Registry

Crossref · 10.1016/j.taap.2013.05.021

Cites

111 of 111 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In ischemic rats treated with PCB, mRNA expression levels of ICAM-1 and CXCL2 were strongly reduced.

    “In ischemic rats treated with PCB, the mRNA expression levels of both markers were strongly reduced [93]”

    From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [93]

  • A similar counteracting effect on Alzheimer's disease may improve ICAM-1- and CXCL2-mediated damage in this disease.

    “suggesting that a similar counteracting effect on AD may improve ICAM-1 and CXCL2-mediated damage in this disease.”

    From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [93]

Cites

Flagged first, then by citations

1 of the 112 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Marín-Prida, J., Pavón-Fuentes, N., Llópiz-Arzuaga, A., Fernández-Massó, J. R., Delgado-Roche, L., Mendoza-Marí, Y., Santana, S. P., Cruz-Ramírez, A., Valenzuela-Silva, C., Nazábal-Gálvez, M., Cintado-Benítez, A., Pardo-Andreu, G. L., Polentarutti, N., Riva, F., Pentón-Arias, E., & Pentón-Rol, G.
Affiliations
  • University of Havana (CU)
  • Centro de Ingeniería Genética y Biotecnología (CU)
  • IRCCS Humanitas Research Hospital (IT)
  • University of Milan (IT)
Venue
Toxicology and Applied Pharmacology
Publisher
Elsevier BV
Published
2013-10
Volume
272
Issue
1
Pages
49-60
ISSN
0041-008X (ISSN-L)
OpenAlex
W1969587909
Cited by
61
References
112
Citations by year
2026: 6 · 2025: 1 · 2024: 8 · 2023: 6 · 2022: 9 · 2021: 8 · 2020: 3 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 6 · 2015: 3 · 2014: 2 · 2013: 1
FWCI
2.23
Type
journal article
Language
en
Open access
closed
Locations
  • Toxicology and Applied Pharmacology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
  • Life Sciences › Neuroscience › Neurology › Neurological Disorders and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heme Oxygenase-1 and Carbon Monoxide
Keywords
phycocyanobilin, phycocyanin, ischemic stroke, cerebral hypoperfusion, neuroprotection, oxidative stress, inflammation, gene expression profiling, common carotid artery occlusion, glutamate-induced neuronal injury, hydrogen peroxide, antioxidant enzymes, lipid peroxidation
MeSH terms
Animals, Brain Chemistry, Cell Survival, Cerebrovascular Disorders, Coloring Agents, Genes, MHC Class II, Hydrogen Peroxide, Inflammation, Male, Oxidation-Reduction, Phycocyanin, Tetrazolium Salts, Thiazoles, Biomarkers, Cytokines, PC12 Cells, Rats, Wistar, Oxidative Stress, Glutamic Acid, Vascular Endothelial Growth Factor A, Microarray Analysis, Rats, Phycobilins, Spirulina, Real-Time Polymerase Chain Reaction

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review