Cited by 118Vitamin D Research StudiesNF-κB Signaling PathwaysImmune Response and Inflammation

RETRACTED: The anti-inflammatory activity of 1,25-dihydroxyvitamin D3 in macrophages

Cohen-Lahav, Merav, Douvdevani, Amos, Chaimovitz, Cidio, Shany, Shraga·2007·The Journal of Steroid Biochemistry and Molecular Biology

Abstract

In a previous study we demonstrated a down-regulatory effect of vitamin D active metabolite (1,25(OH)(2)D(3)) and its vitamin D(2) analog (1,24(OH)(2)D(2)) on TNFalpha expression in macrophages. We also found an inhibitory effect in the physiological concentration (10(-10)M) of 1,25(OH)(2)D(3) which was dose-dependent. This down-regulation, caused by the decrease in NFkappaB activity by 1,25(OH)(2)D(3) and 1,24(OH)(2)D(2), was demonstrated in P388D1 cells transfected with NFkappaB reporter gene (p NFkappaB-Luc) and by EMSA. In our present study we investigated the processes leading to reduced NFkappaB activity on P388D1 cells. A decrease in nuclei NFkappaB-p65 and an increase in cytosolic NFkappaB-p65, were measured, while no changes in total NFkappaB-p65 mRNA and protein levels were observed. Simultaneously, a significant increase in both mRNA and protein levels of the NFkappaB-cytosolic inhibitor, IkappaBalpha, were determined. The half-life of IkappaBalpha-mRNA increased, with a parallel decrease in the phosphorylation of its protein, as the first step of ubiquitinization and degradation. The present results demonstrate that 1,25(OH)(2)D(3) and 1,24(OH)(2)D(2) inhibit TNFalpha expression in macrophages, by increasing IkappaBalpha and decreasing NFkappaB activity. Since NFkappaB is a major transcription factor for TNFalpha and other inflammatory mediators, these findings suggest that 1,25(OH)(2)D(3) and 1,24(OH)(2)D(2) may be used therapeutically as anti-inflammatory agents.

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Crossref · 10.1016/j.jsbmb.2006.12.093This paper is marked retracted.

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14 of 14 references verified0 citation errors · 0 not checkable

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  • Reduced energy intake is paramount compared with the nature of low-energy food; decreased inflammatory markers occur with low-energy fat-rich and low-energy carbohydrate-rich diets.

    “Early studies have shown that reduced energy intake is paramount compared with the nature of low-energy food, i.e. decreased concentrations of inflammatory markers are observed with a low-energy, fat-rich as well as with a low-energy, carbohydrate-rich diet (279) .”

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Authors
Cohen-Lahav, M., Douvdevani, A., Chaimovitz, C., & Shany, S.
Affiliations
Ben-Gurion University of the Negev (IL)
Corresponding author
Shany, Shraga
Venue
The Journal of Steroid Biochemistry and Molecular Biology
Publisher
Elsevier BV
Published
2007-03
Volume
103
Issue
3-5
Pages
558-562
ISSN
0960-0760 (ISSN-L)
OpenAlex
W382757940
Cited by
118
References
14
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 4 · 2020: 2 · 2019: 1 · 2018: 5 · 2017: 6 · 2016: 3 · 2015: 11 · 2014: 8 · 2013: 10 · 2012: 16
FWCI
3.96
Type
journal article
Language
en
Status
Retracted
Open access
closed
Locations
  • The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Vitamin D Research Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
Keywords
1,25-dihydroxyvitamin D3, vitamin D analogs, macrophages, anti-inflammatory activity, TNF-α expression, NF-κB, IκBα, ubiquitination, P388D1 cells, retraction
MeSH terms
NF-KappaB Inhibitor alpha, Calcitriol, Cell Line, Cell Nucleus, Ergocalciferols, Gene Expression Regulation, Macrophages, Phosphorylation, RNA, Messenger, NF-kappa B, Inflammation Mediators, I-kappa B Proteins, Protein Transport

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