Multiplexed steroid profiling of gluco- and mineralocorticoids pathways using a liquid chromatography tandem mass spectrometry method

Travers, Simon; Martinerie, Laetitia; Bouvattier, Claire; Boileau, Pascal; Lombès, Marc; Pussard, Eric

doi:10.1016/j.jsbmb.2016.06.005

Cited by 81Adrenal Hormones and DisordersMetabolomics and Mass Spectrometry StudiesHormonal Regulation and Hypertension

Multiplexed steroid profiling of gluco- and mineralocorticoids pathways using a liquid chromatography tandem mass spectrometry method

Travers, Simon, Martinerie, Laetitia, Bouvattier, Claire, Boileau, Pascal, Lombès, Marc, Pussard, Eric·2017·The Journal of Steroid Biochemistry and Molecular Biology

DOI 10.1016/j.jsbmb.2016.06.005

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Abstract

Serum steroid assays are major tools in the clinical evaluation of adrenal disorders. The main adrenal steroids are routinely measured with immunoassays. However, chromatographic methods are known to offer better specificity. We report a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for simultaneous quantification of 15 adrenal steroids targeting the mineralo- and gluco-corticosteroid pathways. Serum steroids combined with deuterated internal standards were extracted using successive protein precipitation and solid phase extraction steps. Cortisol, cortisone, 11-deoxycortisol, 17-hydroxyprogesterone, 21-deoxycortisol, progesterone, 11-deoxycorticosterone, corticosterone, 11-dehydrocorticosterone, 18-hydroxycorticosterone, 18-hydroxy-11-deoxycorticosterone, aldosterone, dehydroepiandrosterone sulfate, testosterone and androstenedione were resolved in fourteen minutes using a BEH C18 column coupled to a methanol-ammonium formate gradient. Detection was performed using multiple reaction monitoring quantitation. Routinely determined steroid levels by immunoassays were compared to those measured by LC-MS/MS. This method was applied to assess steroid profiles in congenital adrenal hyperplasia (CAH) patients with 21-hydroxylase deficiency. Low quantification limits depending on each steroid (ranging from 0.015ng/mL for aldosterone to 20ng/mL for DHEAS) are adapted to the clinical use. Recoveries of steroids range from 64% for 21-deoxycortisol to 101% for cortisol and are fully corrected by internal standards. A good linearity with R>0.989 is obtained for each compound. The inter-day variation coefficients ranged from 4.7% for cortisol to 16.3% for 11-deoxycorticosterone. The immunoassay for cortisol (Immulite 2000, Siemens) showed acceptable agreement with LC-MS/MS (bias +7.2%). However, Bland-Altman plots revealed large negative bias for aldosterone (-33.4%, AldoCT, CisBio international), for 17-hydroxyprogesterone at concentrations below 2ng/mL (-74.1%, OHP-CT MP Biomedical), for androstenedione (-80.3%, RIA D4, Beckman Coulter) and for 11-deoxycortisol (-125.3%, Diasource Immunoassays). Finally, the analysis of samples from 21-hydroxylase defective patients demonstrated the potential usefulness of multiplexed steroid profiling for the diagnosis and/or monitoring of different forms of congenital adrenal hyperplasia. This LC-MS/MS method provides highly sensitive and specific assessments of mineralo- and glucocorticoids pathways from a small volume sample and is therefore a promising potent tool for clinical and experimental endocrine studies.

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Several sensitive and robust LC-MS/MS methods for multiple steroids have been developed in recent years and many methods meet the requirements for clinical applications.
“Several sensitive and robust LC-MS/MS methods for multiple steroids have been developed in recent years and many methods meet the re quirements for clinical applications [5 -16].”
From Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years (2024) · cites this paper as [7]
Previously published methods including multiple steroid metabolites used larger sample volumes (150-500 μL) and/or longer total run-times (13.5-17 min).
“Previously published methods including multiple steroid metabolites used either larger sample volumes from 150-500 µ L and/or longer total rum-times from 13.5-17 min. [5,7,9,14,22].”
From Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years (2024) · cites this paper as [7]

LC-MS/MS in the Clinical Laboratory - Where to From Here?
Grebe, S. K. G., & Singh, R. J. (2011) · Europe PMC (PubMed Central) · Cited by 356 · see citing works
Tutorial review on validation of liquid chromatography–mass spectrometry methods: Part II
Kruve, A., Rebane, R., Kipper, K., Oldekop, M.-L., Evard, H., Herodes, K., Ravio, P., & Leito, I. (2015) · Analytica Chimica Acta · doi:10.1016/j.aca.2015.02.016 · Cited by 341 · see citing works
Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow
Taylor, A. E., Keevil, B., & Huhtaniemi, I. T. (2015) · European Journal of Endocrinology · doi:10.1530/eje-15-0338 · Cited by 320 · see citing works
Simultaneous determination of 12 steroids by isotope dilution liquid chromatography-photospray ionization tandem mass spectrometry
Guo, T., Taylor, R. L., Singh, R., & Soldin, S. J. (2006) · Clinica Chimica Acta · doi:10.1016/j.cca.2006.03.034 · Cited by 228 · see citing works
Standardized LC–MS/MS based steroid hormone profile-analysis
Koal, T., Schmiederer, D., Pham‐Tuan, H., Röhring, C., & Rauh, M. (2011) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2011.12.001 · Cited by 198 · see citing works
Serum steroid profiling by isotopic dilution-liquid chromatography–mass spectrometry: Comparison with current immunoassays and reference intervals in healthy adults
Fanelli, F., Belluomo, I., Lallo, V. D. D., Cuomo, G., IASIO, R. D., Baccini, M., Casadio, E., Casetta, B., Vicennati, V., Gambineri, A., et al. (2010) · Steroids · doi:10.1016/j.steroids.2010.11.005 · Cited by 190 · see citing works
An LC-MS/MS method for steroid profiling during adrenal venous sampling for investigation of primary aldosteronism
Peitzsch, M., Dekkers, T., Haase, M., Sweep, F. C. G. J., Quack, I., Antoch, G., Siegert, G., Lenders, J. W. M., Deinum, J., Willenberg, H. S., et al. (2015) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2014.10.006 · Cited by 169 · see citing works
Liquid chromatography–tandem mass spectrometry applications in endocrinology
Kushnir, M. M., Rockwood, A. L., & Bergquist, J. (2009) · Mass Spectrometry Reviews · doi:10.1002/mas.20264 · Cited by 159 · see citing works
Novel liquid chromatography tandem mass spectrometry (LC-MS/MS) methods for measuring steroids
Keevil, B. G. (2013) · Best Practice & Research Clinical Endocrinology & Metabolism · doi:10.1016/j.beem.2013.05.015 · Cited by 134 · see citing works
18-Hydroxycorticosterone, 18-Hydroxycortisol, and 18-Oxocortisol in the Diagnosis of Primary Aldosteronism and Its Subtypes
Mulatero, P., di Cella, S. M., Monticone, S., Schiavone, D., Manzo, M. L., Mengozzi, G., Rabbia, F., Terzolo, M., Gómez-Sánchez, E. P., Gómez-Sánchez, C. E., et al. (2012) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2011-2384 · Cited by 133 · see citing works
Measurement of Aldosterone in Human Plasma by Semiautomated HPLC–Tandem Mass Spectrometry
Taylor, P. J., Cooper, D. P., Gordon, R. D., & Stowasser, M. (2009) · Clinical Chemistry · doi:10.1373/clinchem.2008.116004 · Cited by 117 · see citing works
Steroid measurement with LC–MS/MS. Application examples in pediatrics
Rauh, M. (2009) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2009.12.007 · Cited by 105 · see citing works
Multisteroid LC–MS/MS assay for glucocorticoids and androgens and its application in Addison's disease
Methlie, P., Hustad, S., Kellman, R., Almås, B., Erichsen, M. M., Husebye, E. S., & Løvås, K. (2013) · Endocrine Connections · doi:10.1530/ec-13-0023 · Cited by 92 · see citing works
Determination of aldosterone in serum by liquid chromatography–tandem mass spectrometry
Turpeinen, U., Hämäläinen, E. K., & Stenman, U. (2007) · Journal of Chromatography B · doi:10.1016/j.jchromb.2007.11.005 · Cited by 87 · see citing works
Simultaneous determination of endogenous steroid hormones in human and animal plasma and serum by liquid or gas chromatography coupled to tandem mass spectrometry
Abdel‐Khalik, J., Björklund, E., & Hansen, M. A. (2013) · Journal of Chromatography B · doi:10.1016/j.jchromb.2013.03.013 · Cited by 77 · see citing works
Quantitation of aldosterone in human plasma by ultra high performance liquid chromatography tandem mass spectrometry
Hinchliffe, E., Carter, S., Owen, L. J., & Keevil, B. G. (2012) · Journal of Chromatography B · doi:10.1016/j.jchromb.2012.11.013 · Cited by 72 · see citing works
A sensitive and rapid mass spectrometric method for the simultaneous measurement of eight steroid hormones and CALIPER pediatric reference intervals
Kyriakopoulou, L., Yazdanpanah, M., Colantonio, D. A., Chan, M. K., Daly, C. H., & Adeli, K. (2013) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2013.01.002 · Cited by 68 · see citing works
Use of steroid profiles in determining the cause of adrenal insufficiency
Holst, J. P., Soldin, S. J., Tractenberg, R. E., Guo, T., Kundra, P., Verbalis, J. G., & Jonklaas, J. (2007) · Steroids · doi:10.1016/j.steroids.2006.11.001 · Cited by 67 · see citing works
A Reference Method for the Analysis of Aldosterone in Blood by High-Performance Liquid Chromatography–Atmospheric Pressure Chemical Ionization–Tandem Mass Spectrometry
Fredline, V. F., Taylor, P. J., Dodds, H. M., & Johnson, A. G. (1997) · Analytical Biochemistry · doi:10.1006/abio.1997.2340 · Cited by 61 · see citing works
Pediatric reference intervals for aldosterone, 17α-hydroxyprogesterone, dehydroepiandrosterone, testosterone and 25-hydroxy vitamin D3 using tandem mass spectrometry
Soldin, O. P., Sharma, H., Husted, L., & Soldin, S. J. (2009) · Clinical Biochemistry · doi:10.1016/j.clinbiochem.2009.01.015 · Cited by 58 · see citing works
Development of sensitive derivatization method for aldosterone in liquid chromatography–electrospray ionization tandem mass spectrometry of corticosteroids
Yamashita, K., Okuyama, M., Nakagawa, R., Honma, S., Satoh, F., Morimoto, R., Ito, S., Takahashi, M., & Numazawa, M. (2008) · Journal of Chromatography A · doi:10.1016/j.chroma.2008.05.034 · Cited by 57 · see citing works
Determination of serum aldosterone by liquid chromatography and tandem mass spectrometry: a liquid–liquid extraction method for the ABSCIEX API-5000 mass spectrometry system
van der Gugten, J. G., Dubland, J. A., Liu, H., Wang, A. Y., Joseph, C., & Holmes, D. T. (2012) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2011-200564 · Cited by 55 · see citing works
Development and validation of a method using supported liquid extraction for aldosterone determination in human plasma by LC-MS/MS
Meunier, C., Blondelle, D., Faure, P., Baguet, J.-P., Goff, C. M. L., Chabre, O., & Ducros, V. (2015) · Clinica Chimica Acta · doi:10.1016/j.cca.2015.05.007 · Cited by 53 · see citing works
Congenital adrenal hyperplasia – pharmacologic interventions from the prenatal phase to adulthood
Claahsen-van der Grinten, H. L., Stikkelbroeck, N. M. M. L., Otten, B. J., & Hermus, A. R. M. M. (2011) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2011.05.004 · Cited by 53 · see citing works
Hormone Modeling in Preterm Neonates: Establishment of Pituitary and Steroid Hormone Reference Intervals
Greaves, R. F., Pitkin, J., Ho, C. S., Baglin, J., Hunt, R. W., & Zacharin, M. (2015) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-3681 · Cited by 42 · see citing works
Simultaneous quantification of 17α-OH progesterone, 11-deoxycortisol, Δ4-androstenedione, cortisol and cortisone in newborn blood spots using liquid chromatography–tandem mass spectrometry
Magnisali, P., Chalioti, M.-B., Livadara, T., Mataragas, M., Paliatsiou, S., Malamitsi‐Puchner, A., & Moutsatsou, P. (2011) · Journal of Chromatography B · doi:10.1016/j.jchromb.2011.03.048 · Cited by 37 · see citing works
Simultaneous measurement of aldosterone and cortisol by high-performance liquid chromatography–tandem mass spectrometry: Application to dehydration–rehydration studies
Taylor, P. J., van Rosendal, S. P., Coombes, J. S., Gordon, R. D., & Stowasser, M. (2010) · Journal of Chromatography B · doi:10.1016/j.jchromb.2010.03.033 · Cited by 36 · see citing works
Measurement of 18-hydroxycorticosterone during adrenal vein sampling for primary aldosteronism
Auchus, R. J., Chandler, D. W., Singeetham, S., Chokshi, N., Nwariaku, F. E., Dolmatch, B. L., Holt, S. A., Wians, F. H., Josephs, S. C., Trimmer, C. K., et al. (2007) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2006-2631 · Cited by 35 · see citing works
Diagnosis of 21-hydroxylase deficiency by urinary metabolite ratios using gas chromatography–mass spectrometry analysis: reference values for neonates and infants
Kamrath, C., Hartmann, M. F., Boettcher, C., Zimmer, K.-P., & Wudy, S. A. (2016) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2015.10.013 · Cited by 34 · see citing works
LC–MS/MS based determination of basal- and ACTH-stimulated plasma concentrations of 11 steroid hormones: implications for detecting heterozygote CYP21A2 mutation carriers
Kulle, A. E., Riepe, F. G., Hedderich, J., Sippell, W. G., Schmitz, J., Niermeyer, L., & Holterhus, P. (2015) · European Journal of Endocrinology · doi:10.1530/eje-14-1084 · Cited by 30 · see citing works
Mineralocorticoids in congenital adrenal hyperplasia
Biglieri, E. G., & Kater, C. E. (1991) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/0960-0760(91)90268-a · Cited by 29 · see citing works
Liquid chromatography–tandem mass spectrometry analysis of human adrenal vein corticosteroids before and after adrenocorticotropic hormone stimulation
Nakamura, Y., Rege, J., Satoh, F., Morimoto, R., Kennedy, M. R., Ahlem, C. N., Honma, S., Sasano, H., & Rainey, W. E. (2011) · Clinical Endocrinology · doi:10.1111/j.1365-2265.2011.04316.x · Cited by 28 · see citing works
The clinical significance of aldosterone synthase deficiency: report of a novel mutation in the CYP11B2 gene
Hui, E., Yeung, M. C., Cheung, P. T., Kwan, E., Low, L. C. K., Tan, K. C. B., Lam, K. S., & Chan, A. O.-K. (2014) · BMC Endocrine Disorders · doi:10.1186/1472-6823-14-29 · Cited by 28 · see citing works
Supported liquid extraction offers improved sample preparation for aldosterone analysis by liquid chromatography tandem mass spectrometry
van der Gugten, J. G., Crawford, M. L., Grant, R. P., & Holmes, D. T. (2012) · Journal of Clinical Pathology · doi:10.1136/jclinpath-2012-200990 · Cited by 26 · see citing works
Distinctive plasma aldosterone, 18-hydroxycorticosterone, and 18-hydroxydeoxycorticosterone profile in the 21-, 17α-, and 11β-hydroxylase deficiency types of congenital adrenal hyperplasia
Kater, C. E., & Biglieri, E. G. (1983) · The American Journal of Medicine · doi:10.1016/0002-9343(83)91166-x · Cited by 23 · see citing works
Combined quantification of corticotropin-releasing hormone, cortisol-to-cortisone ratio and progesterone by liquid chromatography–Tandem mass spectrometry in placental tissue
Fahlbusch, F. B., Ruebner, M., Rascher, W., & Rauh, M. (2013) · Steroids · doi:10.1016/j.steroids.2013.04.015 · Cited by 22 · see citing works
Development of a solid phase extraction method for the simultaneous determination of steroid hormones in H295R cell line using liquid chromatography–tandem mass spectrometry
Abdel‐Khalik, J., Björklund, E., & Hansen, M. A. (2013) · Journal of Chromatography B · doi:10.1016/j.jchromb.2013.07.013 · Cited by 21 · see citing works
11β-Hydroxysteroid Dehydrogenase Activity in the Brain Does Not Contribute to Systemic Interconversion of Cortisol and Cortisone in Healthy Men
Kilgour, A. H. M., Semple, S., Marshall, I., Andrews, P., Andrew, R., & Walker, B. R. (2014) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2014-3277 · Cited by 16 · see citing works
Chromatographic system for the simultaneous measurement of plasma 18-hydroxy-11-deoxycorticosterone and 18-hydroxycorticosterone by radioimmunoassay: reference data for neonates and infants and its application in aldosterone-synthase deficiency
Riepe, F. G., Krone, N. P., Peter, M., Sippell, W. G., & Partsch, C. (2003) · Journal of Chromatography B · doi:10.1016/s1570-0232(02)00921-2 · Cited by 13 · see citing works

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1 of the 40 references couldn’t be matched to a known paper.

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21-Desoxykortisol
Hubl, W. (2019) · Springer Reference Medizin · doi:10.1007/978-3-662-48986-4_3780 · Cited by 473 · see citing works
From a single steroid to the steroidome: Trends and analytical challenges
Olesti, E., Boccard, J., Visconti, G., González‐Ruiz, V., & Rudaz, S. L. (2020) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2020.105797 · Cited by 74 · see citing works
Applications of Mass Spectrometry for Clinical Diagnostics: The Influence of Turnaround Time
Swiner, D. J., Jackson, S., Burris, B. J., & Badu-Tawiah, A. K. (2020) · Analytical Chemistry · doi:10.1021/acs.analchem.9b04901 · Cited by 73 · see citing works
An LC/MS/MS method for analyzing the steroid metabolome with high accuracy and from small serum samples
Yuan飞袁腾, T.-F., Le, J., Wang, S., & 艳, Y. L. 李. (2020) · Journal of Lipid Research · doi:10.1194/jlr.d119000591 · Cited by 64 · see citing works
A Liquid Chromatography/Tandem Mass Spectometry Profile of 16 Serum Steroids, Including 21-Deoxycortisol and 21-Deoxycorticosterone, for Management of Congenital Adrenal Hyperplasia
Fiet, J., Bouc, Y. L., Guechot, J., Hélin, N., Maubert, M.-A., Farabos, D., & Lamazière, A. (2017) · Journal of the Endocrine Society · doi:10.1210/js.2016-1048 · Cited by 62 · see citing works
Determination of the steroid profile in alternative matrices by liquid chromatography tandem mass spectrometry
Gomez-Gomez, A., Miranda, J., Feixas, G., Arranz Betegon, A., Crispi, F., Gratacós, E., & Pozo, O. J. (2020) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105520 · Cited by 50 · see citing works
Effect of acute and chronic aldosterone exposure on the retinal pigment epithelium-choroid complex in rodents
Canonica, J., Mehanna, C., Bonnard, B., Jonet, L., Gélizé, E., Jaïs, J., Jaisser, F., Zhao, M., & Béhar‐Cohen, F. (2019) · Experimental Eye Research · doi:10.1016/j.exer.2019.107747 · Cited by 45 · see citing works
Sex- and age-specific reference intervals of 16 steroid metabolites quantified simultaneously by LC-MS/MS in sera from 2458 healthy subjects aged 0 to 77 years
Frederiksen, H., Johannsen, T. H., Andersen, S. E., Petersen, J. H., Busch, A. S., Ljubicic, M. L., Fischer, M. B., Upners, E. N., Hagen, C. P., Main, K. M., et al. (2024) · Clinica Chimica Acta · doi:10.1016/j.cca.2024.119852 · Cited by 46 · see citing works
Application of 3D-printed scabbard-like sorbent for sample preparation in bioanalysis expanded to 96-wellplate high-throughput format
Belka, M., Konieczna, L., Okońska, M., Pyszka, M., Ulenberg, S., & Bączek, T. (2019) · Analytica Chimica Acta · doi:10.1016/j.aca.2019.05.078 · Cited by 42 · see citing works
Parallel targeted and non-targeted quantitative analysis of steroids in human serum and peritoneal fluid by liquid chromatography high-resolution mass spectrometry
Andrieu, T., du Toit, T., Vogt, B., Mueller, M. D., & Groessl, M. (2022) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-022-03881-3 · Cited by 41 · see citing works
Comprehensive steroid profiling by liquid chromatography coupled to high resolution mass spectrometry
Kaabia, Z., Laparre, J., Cesbron, N., Bizec, B. L., & Dervilly, G. (2018) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2018.06.003 · Cited by 40 · see citing works
Urinary steroidomic profiles by LC-MS/MS to monitor classic 21-Hydroxylase deficiency
Pussard, E., Travers, S. A. A., Bouvattier, C. M., Xue, Q.-Y., Cosson, C., Viengchareun, S., Martinerie, L., & Lombès, M. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105553 · Cited by 34 · see citing works
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Raff, H. (2016) · Endocrinology · doi:10.1210/en.2016-1500 · Cited by 33 · see citing works
Alterations of adrenal steroidomic profiles in preterm infants at birth
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Billan, F. L., Amazit, L., Kevin Bleakley, A., Xue, Q.-Y., Pussard, E., Lhadj, C., Kolkhof, P., Viengchareun, S., Fagart, J., & Lombès, M. (2018) · The FASEB Journal · doi:10.1096/fj.201800391rr · Cited by 31 · see citing works
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Dried Blood Spot Multiplexed Steroid Profiling Using Liquid Chromatography Tandem Mass Spectrometry in Korean Neonates
Choi, R., Park, H., Oh, H. J., Lee, K., Song, J., & Lee, S. (2019) · Annals of Laboratory Medicine · doi:10.3343/alm.2019.39.3.263 · Cited by 29 · see citing works
Impaired 11β-Hydroxysteroid Dehydrogenase Type 2 in Glucocorticoid-Resistant Patients
Vitellius, G., Delemer, B., Caron, P. J., Chabre, O., Bouligand, J., Pussard, E., Trabado, S., & Lombès, M. (2019) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.2019-00800 · Cited by 29 · see citing works
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Serum steroid profiling by mass spectrometry in adrenocortical tumors: diagnostic implications
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Selective LC-MRM/SIM-MS based profiling of adrenal steroids reveals metabolic signatures of 17α-hydroxylase deficiency
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A novel fully-automated method to measure steroids in serum by liquid chromatography-tandem mass spectrometry
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Zola, M., Bousquet, E., Bourges, J., Azan, F., Zhao, M., Jaworski, T., Pussard, E., & Béhar‐Cohen, F. (2023) · Scientific Reports · doi:10.1038/s41598-023-41126-0 · Cited by 21 · see citing works
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Deng, B., Bi, M., Zheng, Y., Hussain, D., Yang, S., & Chen, D. (2024) · Journal of Analysis and Testing · doi:10.1007/s41664-024-00326-x · Cited by 21 · see citing works
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Dumeige, L., Storey, C., Decourtye, L., Nehlich, M., Lhadj, C., Viengchareun, S., Kappeler, L., Lombès, M., & Martinerie, L. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18020457 · Cited by 16 · see citing works
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Zhang, Q., Liu, R., Xu, H., Yang, X., Zhang, Y., Wang, Q., Gao, P., Kai-shun, B., Han, T., & Li, Q. (2019) · Journal of Chromatography A · doi:10.1016/j.chroma.2019.460723 · Cited by 14 · see citing works
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Denham, S. G., Just, G., Kyle, C. J., Richardson, J., Lee, P. J., Simpson, J. P., Gifford, R. M., Andrew, R., Reynolds, R. M., & Homer, N. Z. M. (2020) · Preprints.org · doi:10.20944/preprints202011.0551.v1 · Cited by 6 · see citing works
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Self-citation
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Hubl, W. (2017) · Springer eBooks · doi:10.1007/978-3-662-49054-9_3780-1
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Honour, J. W. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-818124-9.00003-6
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Honour, J. W. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-818124-9.00021-8
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Honour, J. W. (2023) · Elsevier eBooks · doi:10.1016/b978-0-12-818124-9.00006-1
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Lefèvre, C. R., Vaduva, P., Kvasnička, A., Lenski, M., Senarathne, U. D., Taie, S. F., Zibar, K., & Rolić, T. (2026) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2026-0101
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Dumeige, L. (2019) · doi:10.70675/05bf2142z392dz48dez8223z2fb2756f15dd
Implication du récepteur des glucocorticoïdes en physiopathologie humaine
Vitellius, G. (2019) · doi:10.70675/84630f11zbc82z43a6z8604z69024f4ae7dd
Stéroïdomique corticosurrénalienne par LC-MS/MS : applications en endocrinologie pédiatrique
Travers, S. A. A. (2020) · theses.fr (ABES) · doi:10.70675/84cf9c3bze93ez41dcza251z559f27f3c6a6
Self-citation
Mechanistic links between CSCR and corticoids
Zola, M. (2023) · theses.fr (ABES) · doi:10.70675/3a08e2f8z3b27z4665zb212zbae7666d47d6
Rare Causes of Pediatric Primary Adrenal Insufficiency: An Observational Cohort Study
Schermesser-Sicard, M., Samara‐Boustani, D., Roucher‐Boulez, F., Pinto, G., Carel, J., Quoc, A. N., Vermillac, G., Rovani, S., Thalassinos, C., Costa, S., et al. (2026) · Endocrine Practice · doi:10.1016/j.eprac.2026.05.011
Rapid screening method of glucocorticoid residues in sample-limited cell-cultured meat
Yu, P., Li, S., Zhang, C. H. I., Li, X., Wang, H., & Jin, F. (2026) · Food Research International · doi:10.1016/j.foodres.2026.120146
A simultaneous quantification method of 22 steroid hormones in human serum based on PP-SPE and ID-LC-MS/MS
Yang, S., Wan, W., Qu, Z., Yi, K., Zhang, D., Jiang, Y., Ye, Z., Fang, X., Xie, J., & Dai, X. (2026) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-026-06587-y

The comprehensive characterization of adrenocortical steroidogenesis using two-dimensional ultra-performance liquid chromatography – electrospray ionization tandem mass spectrometry
Karvaly, G., Kovács, K., Mészáros, K. V., Kocsis, I., Patócs, A., & Vásárhelyi, B. (2018) · Journal of Pharmaceutical and Biomedical Analysis · doi:10.1016/j.jpba.2018.01.054 · Cited by 20 · see citing works
Simultaneous quantitation of seven endogenous C-21 adrenal steroids by liquid chromatography tandem mass spectrometry in human serum
Carvalho, V. M., Nakamura, O. H., & Vieira, J. G. H. (2008) · Journal of Chromatography B · doi:10.1016/j.jchromb.2008.07.035 · Cited by 68 · see citing works
Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) Method for the Quantification of Steroids Androstenedione, Dehydroepiandrosterone, 11-Deoxycortisol, 17-Hydroxyprogesterone, and Testosterone
Munar, A., Frazee, C. C., & Garg, U. C. (2022) · Methods in molecular biology · doi:10.1007/978-1-0716-2565-1_40 · Cited by 4 · see citing works
Multisteroid LC–MS/MS assay for glucocorticoids and androgens and its application in Addison's disease
Methlie, P., Hustad, S., Kellman, R., Almås, B., Erichsen, M. M., Husebye, E. S., & Løvås, K. (2013) · Endocrine Connections · doi:10.1530/ec-13-0023 · Cited by 92 · see citing works
Principles and clinical applications of liquid chromatography - tandem mass spectrometry for the determination of adrenal and gonadal steroid hormones.
Kulle, A. E., Welzel, M., Holterhus, P.-M., & Riepe, F. G. (2011) · PubMed · doi:10.3275/7843 · Cited by 31 · see citing works

Authors: Travers, S., Martinerie, L., Bouvattier, C., Boileau, P., Lombès, M., & Pussard, E.
Affiliations: Université Paris-Sud (FR)
Université de Versailles Saint-Quentin-en-Yvelines (FR)
Corresponding author: Pussard, Eric
ORCID: Travers, Simon
Bouvattier, Claire
Boileau, Pascal
Lombès, Marc
Pussard, Eric
Venue: The Journal of Steroid Biochemistry and Molecular Biology
Publisher: Elsevier BV
Published: 2017-01
Volume: 165
Issue: Pt B
Pages: 202-211
ISSN: 0960-0760 (ISSN-L)
DOI: 10.1016/j.jsbmb.2016.06.005
OpenAlex: W2473953302
Cited by: 81
References: 40
Citations by year: 2026: 8 · 2025: 3 · 2024: 4 · 2023: 11 · 2022: 6 · 2021: 8 · 2020: 7 · 2019: 20 · 2018: 4 · 2017: 9 · 2016: 1
FWCI: 11.38
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: The Journal of Steroid Biochemistry and Molecular Biology (publishedVersion)
PubMed (publishedVersion)
HAL (submittedVersion)
Funders: Institut National de la Santé et de la Recherche Médicale
Cardiovasculaire-Obésité-Rein-Diabète-Domaine d’Intérêt Majeur
Waters Xevo TQS triple-quadrupole mass spectrometer
Valerie Steiner and Cécile Dupuy for their advice in the first steps of method development
Topics: Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Hormonal Regulation and Hypertension
Keywords: adrenal steroids, mineralocorticoids, glucocorticoids, congenital adrenal hyperplasia, 21-hydroxylase deficiency, cortisol, aldosterone, 17-hydroxyprogesterone, dehydroepiandrosterone sulfate, androstenedione, adrenal disorders, immunoassay comparison
MeSH terms: Adrenal Hyperplasia, Congenital, Child, Chromatography, High Pressure Liquid, Chromatography, Liquid, Glucocorticoids, Humans, Immunoassay, Mineralocorticoids, Mutation, Regression Analysis, Steroids, Reproducibility of Results, Tandem Mass Spectrometry, Limit of Detection

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Multiplexed steroid profiling of gluco- and mineralocorticoids pathways using a liquid chromatography tandem mass spectrometry method

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