The DOI matches its Crossref record; 28 of the 29 references it cites were found in a registry or an index.
Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells
Open accessAbstract
Objective We compared 3 different decellularization protocols in porcine heart valves for efficiency of complete cell removal and potential for recellularization. Methods Porcine aortic and pulmonary roots were treated with trypsin, sodium-dodecyl-sulphate, or a new method using 0.25% tert-octylphenyl-polyoxyethylen in combination with sodium-deoxycholate. After a subsequent ribonuclease digestion, specimens were seeded with in vitro expanded human saphenous vein endothelial cells and myofibroblasts. Results After treatment with trypsin and subsequent ribonuclease digestion, endothelial attachment took place; however, xenogenic cells were still visible within the matrix. Unexpectedly, when human cells were seeded onto specimens that had been decellularized with sodium-dodecyl-sulphate, the matrices were surrounded by nonviable endothelial cell fragments, indicating a toxic influence of the ionic detergent; 0.25% tert-octylphenyl-polyoxyethylen together with sodium-deoxycholate completely removed porcine cells and enabled host recellularization. Conclusion Compared with trypsin and sodium-dodecyl-sulphate involving decellularization procedures, reported to be effective in cell removal and susceptible to recellularization with human cells, only the porcine matrix treated with a new detergent-based decellularization method using 0.25% tert-octylphenyl-polyoxyethylen/sodium-deoxycholate followed by nuclease digestion presented an excellent scaffold for recellularization with human cells.
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“Initially used in decellularization for its efficacy in disrupting tissue ultrastructure and enhancing the penetration of other agents, trypsin may nevertheless impact ECM mechanics.”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [44]
In the citing paper
“Viral contamination is the substantial cause of ribonuclease treatment in some decellularization protocols [99].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [99]
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Metadata
- Authors
- Rieder, E., Kasimir, M.-T., Silberhumer, G., Seebacher, G., Wolner, E., Simon, P., & Weigel, G.
- Affiliations
- University of Vienna (AT)
- Corresponding author
- Weigel, Guenter
- Venue
- The Journal of Thoracic and Cardiovascular Surgery
- Publisher
- Elsevier BV
- Published
- 2004-02
- Volume
- 127
- Issue
- 2
- Pages
- 399-405
- ISSN
- 0022-5223 (ISSN-L)
- OpenAlex
- W2109385127
- Cited by
- 430
- References
- 29
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 21 · 2023: 21 · 2022: 30 · 2021: 23 · 2020: 25 · 2019: 25 · 2018: 17 · 2017: 28 · 2016: 22 · 2015: 22 · 2014: 26 · 2013: 24 · 2012: 16
- FWCI
- 11.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Thoracic and Cardiovascular Surgery (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- decellularization, porcine valves, recellularization, sodium deoxycholate, sodium dodecyl sulfate, trypsin, ribonuclease digestion, aortic valve, pulmonary valve, endothelial cells, myofibroblasts, detergent toxicity
- MeSH terms
- Aortic Valve, Biocompatible Materials, Bioprosthesis, Chelating Agents, Deoxycholic Acid, Deoxyribonucleases, Detergents, Edetic Acid, Endothelium, Vascular, Extracellular Matrix, Heart Valve Prosthesis, Humans, Microscopy, Electron, Prosthesis Design, Pulmonary Valve, Ribonucleases, Saphenous Vein, Sodium Dodecyl Sulfate, Surface-Active Agents, Octoxynol
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