The DOI matches its Crossref record; 28 of the 29 references it cites were found in a registry or an index.
Decellularization protocols of porcine heart valves differ importantly in efficiency of cell removal and susceptibility of the matrix to recellularization with human vascular cells
Open accessAbstract
Objective We compared 3 different decellularization protocols in porcine heart valves for efficiency of complete cell removal and potential for recellularization. Methods Porcine aortic and pulmonary roots were treated with trypsin, sodium-dodecyl-sulphate, or a new method using 0.25% tert-octylphenyl-polyoxyethylen in combination with sodium-deoxycholate. After a subsequent ribonuclease digestion, specimens were seeded with in vitro expanded human saphenous vein endothelial cells and myofibroblasts. Results After treatment with trypsin and subsequent ribonuclease digestion, endothelial attachment took place; however, xenogenic cells were still visible within the matrix. Unexpectedly, when human cells were seeded onto specimens that had been decellularized with sodium-dodecyl-sulphate, the matrices were surrounded by nonviable endothelial cell fragments, indicating a toxic influence of the ionic detergent; 0.25% tert-octylphenyl-polyoxyethylen together with sodium-deoxycholate completely removed porcine cells and enabled host recellularization. Conclusion Compared with trypsin and sodium-dodecyl-sulphate involving decellularization procedures, reported to be effective in cell removal and susceptible to recellularization with human cells, only the porcine matrix treated with a new detergent-based decellularization method using 0.25% tert-octylphenyl-polyoxyethylen/sodium-deoxycholate followed by nuclease digestion presented an excellent scaffold for recellularization with human cells.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Initially used in decellularization for its efficacy in disrupting tissue ultrastructure and enhancing the penetration of other agents, trypsin may nevertheless impact ECM mechanics.”
From Bioactive scaffolds for tissue engineering: A review of decellularized extracellular matrix applications and innovations (2025) · cites this paper as [44]
In the citing paper
“Viral contamination is the substantial cause of ribonuclease treatment in some decellularization protocols [99].”
From Immunogenicity of decellularized extracellular matrix scaffolds: a bottleneck in tissue engineering and regenerative medicine (2023) · cites this paper as [99]
Citations
Cites
Jaffe, E. A., Nachman, R., Becker, C. G., & Minick, C. (1973) · Journal of Clinical Investigation · doi:10.1172/jci107470 · Cited by 7,146
Thromboembolic and bleeding complications in patients with mechanical heart valve prostheses.
Cannegieter, S. C., Rosendaal, F. R., & Briët, E. (1994) · Circulation · doi:10.1161/01.cir.89.2.635 · Cited by 1,072
Infection of human cells by an endogenous retrovirus of pigs
Patience, C., Takeuchi, Y., & Weiss, R. A. (1997) · Nature Medicine · doi:10.1038/nm0397-282 · Cited by 953
Early failure of the tissue engineered porcine heart valve SYNERGRAFT™ in pediatric patients
Simon, P. (2003) · European Journal of Cardio-Thoracic Surgery · doi:10.1016/s1010-7940(03)00094-0 · Cited by 627
Self-citationTissue Engineering Heart Valves: Valve Leaflet Replacement Study in a Lamb Model
Shinoka, T., Breuer, C. K., Tanel, R. E., Zünd, G., Miura, T., Ma, P. X., Langer, R., Vacanti, J. P., & Mayer, J. E. (1995) · The Annals of Thoracic Surgery · doi:10.1016/0003-4975(95)00733-4 · Cited by 450
Creation Of Viable Pulmonary Artery Autografts Through Tissue Engineering
Shinoka, T., Shum‐Tim, D., Ma, P. X., Tanel, R. E., Isogai, N., Langer, R., Vacanti, J. P., & Mayer, J. E. (1998) · Journal of Thoracic and Cardiovascular Surgery · doi:10.1016/s0022-5223(98)70315-0 · Cited by 445
Steinhoff, G., Stock, U., Karim, N., Mertsching, H., Timke, A., Meliß, R. R., Pethig, K., Haverich, A., & Bader, A. (2000) · Circulation · doi:10.1161/01.cir.102.suppl_3.iii-50 · Cited by 352
Bader, A., Schilling, T., Teebken, O. E., Brandes, G., Herden, T., Steinhoff, G., & Haverich, A. (1998) · European Journal of Cardio-Thoracic Surgery · doi:10.1016/s1010-7940(98)00171-7 · Cited by 340
Martin, U., Kiessig, V., Blusch, J., Haverich, A., von der Helm, K., Herden, T., & Steinhoff, G. (1998) · The Lancet · doi:10.1016/s0140-6736(98)07144-x · Cited by 311
Clinical autologous in vitro endothelialization of 153 infrainguinal ePTFE grafts
Meinhart, J., Deutsch, M., Fischlein, T., Howanietz, N., Fröschl, A., & Zilla, P. (2001) · The Annals of Thoracic Surgery · doi:10.1016/s0003-4975(01)02555-3 · Cited by 263
Research works printed but not linked: 2
- Booth, 2002, Tissue engineering of cardiac valve prosthesis I, J Heart Valve Dis, 11, 457
- O'Brien, 1999, The Synergraft valve, Semin Thorac Cardiovasc Surg, 11, 194
Cited by
Sorted by citations
An overview of tissue and whole organ decellularization processes
Crapo, P. M., Gilbert, T. W., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.01.057 · Cited by 3,591
On the mechanisms of biocompatibility
Williams, D. F. (2008) · Biomaterials · doi:10.1016/j.biomaterials.2008.04.023 · Cited by 2,772
Perfusion-decellularized matrix: using nature's platform to engineer a bioartificial heart
Ott, H. C., Matthiesen, T. S., Goh, S.-K., Black, L. D., Kren, S. M., Netoff, T. I., & Taylor, D. A. (2008) · Nature Medicine · doi:10.1038/nm1684 · Cited by 2,650
Decellularization of tissues and organs
Gilbert, T. W., Sellaro, T. L., & Badylak, S. F. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.02.014 · Cited by 2,222
Reprint of: Extracellular matrix as a biological scaffold material: Structure and function
Badylak, S. F., Freytes, D. O., & Gilbert, T. W. (2015) · Acta Biomaterialia · doi:10.1016/j.actbio.2015.07.016 · Cited by 1,367
Badylak, S. F., Taylor, D., & Uygun, K. (2011) · Annual Review of Biomedical Engineering · doi:10.1146/annurev-bioeng-071910-124743 · Cited by 1,041
Decellularization Strategies for Regenerative Medicine: From Processing Techniques to Applications
Gilpin, A., & Yang, Y. (2017) · BioMed Research International · doi:10.1155/2017/9831534 · Cited by 742
Methods of tissue decellularization used for preparation of biologic scaffolds and in vivo relevance
Keane, T. J., Swinehart, I. T., & Badylak, S. F. (2015) · Methods · doi:10.1016/j.ymeth.2015.03.005 · Cited by 670
Consequences of ineffective decellularization of biologic scaffolds on the host response
Keane, T. J., Londono, R., Turner, N. J., & Badylak, S. F. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2011.10.054 · Cited by 613
Extracellular matrix-based biomaterial scaffolds and the host response
Aamodt, J. M., & Grainger, D. W. (2016) · Biomaterials · doi:10.1016/j.biomaterials.2016.02.003 · Cited by 509
Reing, J. E., Brown, B. N., Daly, K. A., Freund, J. M., Gilbert, T. W., Hsiong, S. X., Huber, A., Kullas, K. E., Tottey, S., Wolf, M. T., et al. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.07.083 · Cited by 475
Kim, B. S., Das, S., Jang, J., & Cho, D.-W. (2020) · Chemical Reviews · doi:10.1021/acs.chemrev.9b00808 · Cited by 465
Flynn, L. E. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.02.046 · Cited by 441
Woods, T., & Gratzer, P. F. (2005) · Biomaterials · doi:10.1016/j.biomaterials.2005.05.066 · Cited by 381
Kasravi, M., Ahmadi, A., Babajani, A., Mazloomnejad, R., Hatamnejad, M. R., Shariatzadeh, S., Bahrami, S., & Niknejad, H. (2023) · Biomaterials Research · doi:10.1186/s40824-023-00348-z · Cited by 316
Heart Valve Tissue Engineering
Veselý, I. (2005) · Circulation Research · doi:10.1161/01.res.0000185326.04010.9f · Cited by 309
Badylak, S. F. (2014) · Annals of Biomedical Engineering · doi:10.1007/s10439-013-0963-7 · Cited by 307
Mendibil, U., Ruiz-Hernández, R., Retegi-Carrión, S., García‐Urkía, N., Olalde, B., & Abarrategi, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21155447 · Cited by 323
Neishabouri, A., Soltani Khaboushan, A., Daghigh, F., Kajbafzadeh, A.-M., & Majidi Zolbin, M. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.805299 · Cited by 281
Perfusion decellularization of whole organs
Guyette, J. P., Gilpin, S. E., Charest, J. M., Tapias, L. F., Ren, X., & Ott, H. C. (2014) · Nature Protocols · doi:10.1038/nprot.2014.097 · Cited by 275
Cebotari, S., Tudorache, I., Jaekel, T., Hilfiker, A., Dorfman, S. E., Ternes, W., Haverich, A., & Lichtenberg, A. (2010) · Artificial Organs · doi:10.1111/j.1525-1594.2009.00796.x · Cited by 275
The use of high-hydrostatic pressure treatment to decellularize blood vessels
Funamoto, S., Nam, K., Kimura, T., Murakoshi, A., Hashimoto, Y., Niwaya, K., Kitamura, S., Fujisato, T., & Kishida, A. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.01.073 · Cited by 274
The impact of detergents on the tissue decellularization process: A ToF-SIMS study
White, L. J., Taylor, A. J., Faulk, D. M., Keane, T. J., Saldin, L. T., Reing, J. E., Swinehart, I. T., Turner, N. J., Ratner, B. D., & Badylak, S. F. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2016.12.033 · Cited by 268
Medical Application of Microbial Biopolyesters Polyhydroxyalkanoates
Wu, Q., Wang, Y., & Chen, G. (2009) · Artificial Cells Blood Substitutes and Biotechnology · doi:10.1080/10731190802664429 · Cited by 227
Peripheral nerve conduits: technology update
Hasirci, V., Arslantunali, D., Dursun, T., Yucel, D., & Hasirci, N. (2014) · Medical Devices Evidence and Research · doi:10.2147/mder.s59124 · Cited by 224
Brown, B. N., Freund, J. M., Li, H., Rubin, J. P., Reing, J. E., Jeffries, E. M., Wolf, M. T., Tottey, S., Barnes, C. A., Ratner, B. D., et al. (2010) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2010.0342 · Cited by 223
Caralt, M., Uzarski, J. S., Iacob, S., Obergfell, K. P., Berg, N., Bijonowski, B. M., Kiefer, K. M., Ward, H. H., Wandinger‐Ness, A., Miller, W. M., et al. (2014) · American Journal of Transplantation · doi:10.1111/ajt.12999 · Cited by 224
Wang, Y., Cui, C.-B., Yamauchi, M., Miguez, P., Roach, M., Malavarca, R., Costello, J. M., Cardinale, V., Wauthier, E., Barbier, C., et al. (2011) · Hepatology · doi:10.1002/hep.24012 · Cited by 220
Biocompatibility evaluation of tissue-engineered decellularized scaffolds for biomedical application
Hussein, K. H., Park, K.-M., Kang, K.-S., & Woo, H.-M. (2016) · Materials Science and Engineering: C · doi:10.1016/j.msec.2016.05.068 · Cited by 218
Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture
Modulevsky, D. J., Lefebvre, C., Haase, K., Al‐Rekabi, Z., & Pelling, A. E. (2014) · PLoS ONE · doi:10.1371/journal.pone.0097835 · Cited by 217
Aortic valve disease and treatment: The need for naturally engineered solutions
Butcher, J. T., Mahler, G. J., & Hockaday, L. A. (2011) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2011.01.008 · Cited by 220
Yang, B., Zhang, Y., Zhou, L., Sun, Z., Zheng, J., Chen, Y., & Dai, Y. (2010) · Tissue Engineering Part C: Methods · doi:10.1089/ten.tec.2009.0311 · Cited by 206
Lam, D., Enright, H. A., Cadena, J., Peters, S. K. G., Sales, A. P., Osburn, J. J., Soscia, D. A., Kulp, K. S., Wheeler, E. K., & Fischer, N. O. (2019) · Scientific Reports · doi:10.1038/s41598-019-40128-1 · Cited by 213
Extracellular Matrix-Based Biomaterials and Their Influence Upon Cell Behavior
Cramer, M. C., & Badylak, S. F. (2020) · Annals of Biomedical Engineering · doi:10.1007/s10439-019-02408-9 · Cited by 196
The host response to naturally-derived extracellular matrix biomaterials
Morris, A. H., Stamer, D. K., & Kyriakides, T. R. (2017) · Seminars in Immunology · doi:10.1016/j.smim.2017.01.002 · Cited by 180
Dijkman, P. E., Driessen‐Mol, A., Frese, L., Hoerstrup, S. P., & Baaijens, F. F. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.03.015 · Cited by 172
Tissue Engineering of Heart Valves
Rieder, E., Seebacher, G., Kasimir, M., Eichmair, E., Winter, B., Dekan, B., Wolner, E., Simon, P., & Weigel, G. (2005) · Circulation · doi:10.1161/circulationaha.104.473629 · Cited by 171
Self-citationScaffolds for tissue engineering of cardiac valves
Jana, S., Tefft, B. J., Spoon, D. B., & Simari, R. D. (2014) · Acta Biomaterialia · doi:10.1016/j.actbio.2014.03.014 · Cited by 168
Jana, S., & Lerman, A. (2015) · Biotechnology Advances · doi:10.1016/j.biotechadv.2015.07.006 · Cited by 164
Gratzer, P. F., Harrison, R. D., & Woods, T. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.2975 · Cited by 160
Decellularization and Recellularization Technologies in Tissue Engineering
Fu, R., Wang, Y.-C., Liu, S., Shih, T.-R., Lin, H.-L., Chen, Y.-M., Sung, J.-H., Lu, C.-H., Wei, J.-R., Wang, Z.-W., et al. (2014) · Cell Transplantation · doi:10.3727/096368914x678382 · Cited by 165
Development and Characterization of an Acellular Human Pericardial Matrix for Tissue Engineering
Mirsadraee, S., Wilcox, H. E., Korossis, S. A., Kearney, J. N., Watterson, K. G., Fisher, J., & Ingham, E. (2006) · Tissue Engineering · doi:10.1089/ten.2006.12.763 · Cited by 157
Decellularized matrices for cardiovascular tissue engineering.
Moroni, F., & Mirabella, T. (2014) · Europe PMC (PubMed Central) · Cited by 149
(2006) · PubMed · Cited by 149
Self-citationTissue engineering of heart valves: advances and current challenges
Mol, A., Smits, A. I. P. M., Bouten, C. V. C., & Baaijens, F. F. (2009) · Expert Review of Medical Devices · doi:10.1586/erd.09.12 · Cited by 147
A Biodegradable, Acellular Xenogeneic Scaffold for Regeneration of the Vocal Fold Lamina Propria
Xu, C. C., Chan, R. W., & Tirunagari, N. (2007) · Tissue Engineering · doi:10.1089/ten.2006.0169 · Cited by 148
Decellularized extracellular matrix mediates tissue construction and regeneration
Liu, C., Pei, M., Li, Q., & Zhang, Y. (2021) · Frontiers of Medicine · doi:10.1007/s11684-021-0900-3 · Cited by 151
Solarte-David, V. A., Güiza-Argüello, V. R., Arango-Rodríguez, M. L., Sossa, C., & Becerra‐Bayona, S. M. (2022) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2022.821852 · Cited by 150
The effect of source animal age upon extracellular matrix scaffold properties
Tottey, S., Johnson, S. A., Crapo, P. M., Reing, J. E., Zhang, L., Jiang, H., Medberry, C. J., Reines, B., & Badylak, S. F. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2010.09.006 · Cited by 138
Wilshaw, S.-P., Kearney, J., Fisher, J., & Ingham, E. (2008) · Tissue Engineering Part A · doi:10.1089/tea.2007.0145 · Cited by 137
Lichtenberg, A., Tudorache, I., Cebotari, S., Ringes‐Lichtenberg, S., Sturz, G., Hoeffler, K., Hurschler, C., Brandes, G., Hilfiker, A., & Haverich, A. (2006) · Biomaterials · doi:10.1016/j.biomaterials.2006.03.047 · Cited by 135
Costa, A., Naranjo, J. D., Londono, R., & Badylak, S. F. (2017) · Cold Spring Harbor Perspectives in Medicine · doi:10.1101/cshperspect.a025676 · Cited by 135
Regulation of decellularized matrix mediated immune response
Chakraborty, J., Roy, S., & Ghosh, S. (2020) · Biomaterials Science · doi:10.1039/c9bm01780a · Cited by 130
TRITON‐X is most effective among three decellularization agents for ACL tissue engineering
Vavken, P., Joshi, S. M., & Murray, M. M. (2009) · Journal of Orthopaedic Research® · doi:10.1002/jor.20932 · Cited by 134
Liu, J., Song, Q., Yin, W., Li, C., An, N., Le, Y., Wang, Q., Feng, Y., Hu, Y., & Wang, Y. (2025) · Exploration · doi:10.1002/exp.20230078 · Cited by 140
Wang, Y., Bao, J., Wu, Q., Zhou, Y., Li, Y., Wu, X., Shi, Y., Li, L., & Bu, H. (2015) · Xenotransplantation · doi:10.1111/xen.12141 · Cited by 125
Recellularization of Decellularized Allograft Scaffolds in Ovine Great Vessel Reconstructions
Ketchedjian, A., Jones, A. L., Krueger, P. M., Robinson, E., Crouch, K., Wolfinbarger, L., & Hopkins, R. A. (2005) · The Annals of Thoracic Surgery · doi:10.1016/j.athoracsur.2004.09.033 · Cited by 118
Recellularization of decellularized heart valves: Progress toward the tissue-engineered heart valve
VeDepo, M. C., Detamore, M. S., Hopkins, R. A., & Converse, G. L. (2017) · Journal of Tissue Engineering · doi:10.1177/2041731417726327 · Cited by 117
Pridgen, B. C., Woon, C. Y.-L., Kim, M. Y., Thorfinn, J., Lindsey, D. P., Pham, H. M., & Chang, J. (2011) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2010.0457 · Cited by 115
Systematic in vitro comparison of decellularization protocols for blood vessels
Simsa, R., Padma, A. M., Heher, P., Hellström, M., Teuschl, A., Jenndahl, L., Bergh, N., & Fogelstrand, P. (2018) · PLOS ONE · doi:10.1371/journal.pone.0209269 · Cited by 115
Functional acellular matrix for tissue repair
Wang, B., Qinglai, T., Yang, Q., Li, M., Zeng, S., Yang, X., Xiao, Z., Tong, X., Lei, L., & Li, S. (2022) · Materials Today Bio · doi:10.1016/j.mtbio.2022.100530 · Cited by 111
Sellaro, T. L., Hildebrand, D. K., Lu, Q., Vyavahare, N. R., Scott, M., & Sacks, M. S. (2006) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.30871 · Cited by 110
Novel Utilization of Serum in Tissue Decellularization
Gui, L., Chan, S. A., Breuer, C. K., & Niklason, L. E. (2010) · Tissue Engineering Part C: Methods · doi:10.1089/ten.tec.2009.0120 · Cited by 109
Heath, D. E. (2019) · Regenerative Engineering and Translational Medicine · doi:10.1007/s40883-018-0080-0 · Cited by 110
Yang, M., Chen, C., Wang, X., Zhu, Y., & Gu, Y. (2009) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.31409 · Cited by 108
Chun, S. Y., Lim, J. O., Lee, E. H., Han, M., Ha, Y., Lee, J. N., Kim, B. S., Park, M., Yeo, M., Jung, B., et al. (2019) · Tissue Engineering and Regenerative Medicine · doi:10.1007/s13770-019-00199-7 · Cited by 107
Biocompatibility of Acellular Human Pericardium
Mirsadraee, S., Wilcox, H. E., Watterson, K. G., Kearney, J. N., Hunt, J., Fisher, J., & Ingham, E. (2007) · Journal of Surgical Research · doi:10.1016/j.jss.2007.01.026 · Cited by 104
Using extracellular matrix for regenerative medicine in the spinal cord
Volpato, F. Z., Führmann, T., Migliaresi, C., Hutmacher, D. W., & Dalton, P. D. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.03.057 · Cited by 103
Youngstrom, D. W., Barrett, J. G., Jose, R. R., & Kaplan, D. L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0064151 · Cited by 102
The decellularized porcine heart valve matrix in tissue engineering
Kasimir, M., Weigel, G., Sharma, J., Rieder, E., Seebacher, G., Wolner, E., & Simon, P. (2005) · Thrombosis and Haemostasis · doi:10.1160/th05-01-0025 · Cited by 101
Self-citationMechanical Evaluation of Decellularized Porcine Thoracic Aorta
Zou, Y. F., & Zhang, Y. (2011) · Journal of Surgical Research · doi:10.1016/j.jss.2011.03.070 · Cited by 100
Bone Marrow Niches of Hematopoietic Stem and Progenitor Cells
Kandarakov, O. F., Belyavsky, A. V., & Semenova, E. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms23084462 · Cited by 107
The surface molecular functionality of decellularized extracellular matrices
Barnes, C. A., Brison, J., Michel, R., Brown, B. N., Castner, D. G., Badylak, S. F., & Ratner, B. D. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.09.007 · Cited by 99
Tracheal Replacement With Cryopreserved, Decellularized, or Glutaraldehyde-Treated Aortic Allografts
Seguin‐Givelet, A., Radu, D. M., Holder‐Espinasse, M., Bruneval, P., Fialaire, A., Duterque‐Coquillaud, M., Carpentier, A., & Martinod, E. (2009) · The Annals of Thoracic Surgery · doi:10.1016/j.athoracsur.2008.11.038 · Cited by 97
Tissue engineering of heart valves using decellularized xenogeneic or polymeric starter matrices
Schmidt, D., Stock, U. A., & Hoerstrup, S. P. (2007) · Philosophical Transactions of the Royal Society B Biological Sciences · doi:10.1098/rstb.2007.2131 · Cited by 92
Nagao, R. J., Xu, J. H., Luo, P., Xue, J., Wang, Y., Kotha, S. S., Zeng, W., Fu, X., Himmelfarb, J., & Zheng, Y. Y. (2016) · Tissue Engineering Part A · doi:10.1089/ten.tea.2016.0213 · Cited by 90
Tissue and organ decellularization in regenerative medicine
Damodaran, R. G., & Vermette, P. (2018) · Biotechnology Progress · doi:10.1002/btpr.2699 · Cited by 91
Effect of Decellularization Protocol on the Mechanical Behavior of Porcine Descending Aorta
Fitzpatrick, J. C., Clark, P., & Capaldi, F. M. (2010) · International Journal of Biomaterials · doi:10.1155/2010/620503 · Cited by 84
Barbulescu, G. I., Bojin, F. M., Ordodi, V. L., Goje, I.-D., Barbulescu, A. S., & Păunescu, V. (2022) · International Journal of Molecular Sciences · doi:10.3390/ijms232113040 · Cited by 89
Decellularized bovine intervertebral disc as a natural scaffold for xenogenic cell studies
Chan, L. K. Y., Leung, V., Tam, V., Lu, W. W., Sze, K. Y., & Cheung, K. (2012) · Acta Biomaterialia · doi:10.1016/j.actbio.2012.09.005 · Cited by 84
Hülsmann, J., Grün, K., Amouri, S. E., Barth, M., Hornung, K., Holzfuß, C., Lichtenberg, A., & Akhyari, P. (2012) · Xenotransplantation · doi:10.1111/j.1399-3089.2012.00719.x · Cited by 81
Design and Characterization of Tissue-Specific Extracellular Matrix-Derived Microcarriers
Turner, A. E. B., & Flynn, L. E. (2011) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2011.0246 · Cited by 79
Jin, Q., Liu, G., Li, S., Yuan, H., Yun, Z., Zhang, W., Zhang, S., Dai, Y., & Yu-zhen, M. (2018) · Journal of Cellular Physiology · doi:10.1002/jcp.26782 · Cited by 79
Tissue engineering on matrix: future of autologous tissue replacement
Weber, B., Emmert, M. Y., Schoenauer, R., Brokopp, C. E., Baumgartner, L., & Hoerstrup, S. P. (2011) · Seminars in Immunopathology · doi:10.1007/s00281-011-0258-8 · Cited by 78
Generation and characterization of a human acellular meniscus scaffold for tissue engineering
Sandmann, G. H., Eichhorn, S., Vogt, S., Adamczyk, C., Aryee, S., Hoberg, M., Milz, S., Imhoff, A. B., & Tischer, T. (2008) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.32269 · Cited by 75
Development of a novel human recellularized endometrium that responds to a 28-day hormone treatment†
Olalekan, S. A., Burdette, J. E., Getsios, S., Woodruff, T. K., & Kim, J. J. (2017) · Biology of Reproduction · doi:10.1093/biolre/iox039 · Cited by 72
Biomechanical characterization of decellularized and cross-linked bovine pericardium.
Oswal, D., Korossis, S., Mirsadraee, S., Wilcox, H., Watterson, K. G., Fisher, J., & Ingham, E. (2007) · PubMed · Cited by 72
Biomaterials to Prevascularize Engineered Tissues
Tian, L., & George, S. C. (2011) · Journal of Cardiovascular Translational Research · doi:10.1007/s12265-011-9301-3 · Cited by 72
In vitro analysis of an allogenic scaffold for tissue‐engineered meniscus replacement
Maier, D., Braeun, K., Steinhauser, E., Ueblacker, P., Oberst, M., Kreuz, P., Roos, N., Martinek, V., & Imhoff, A. B. (2007) · Journal of Orthopaedic Research® · doi:10.1002/jor.20405 · Cited by 69
Schenke‐Layland, K., Rofail, F., Heydarkhan, S., Gluck, J. M., Ingle, N. P., Angelis, E., Choi, C., MacLellan, W. R., Beygui, R. E., Shemin, R. J., et al. (2009) · Biomaterials · doi:10.1016/j.biomaterials.2009.05.033 · Cited by 67
Different approaches to heart valve decellularization: A comprehensive overview of the past 30 years
Naso, F., & Gandaglia, A. (2017) · Xenotransplantation · doi:10.1111/xen.12354 · Cited by 66
Kumar, A., Sood, A., & Han, S. S. (2022) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2022.2132206 · Cited by 66
Yuan, M., Yeung, C. W., Li, Y. Y., Diao, H., Cheung, K., Chan, D. T.-M., Cheah, K. S. E., & Chan, B. P. (2013) · Biomaterials · doi:10.1016/j.biomaterials.2013.02.004 · Cited by 66
Wu, Q., Bao, J., Zhou, Y., Wang, Y., Du, Z., Shi, Y., Li, L., & Bu, H. (2015) · BioMed Research International · doi:10.1155/2015/785474 · Cited by 67
Approaches to heart valve tissue engineering scaffold design
Brody, S., & Pandit, A. S. (2007) · Journal of Biomedical Materials Research Part B Applied Biomaterials · doi:10.1002/jbm.b.30763 · Cited by 66
Medical applications of biopolyesters polyhydroxyalkanoates
Chen, G., & Wang, Y. (2013) · Chinese Journal of Polymer Science · doi:10.1007/s10118-013-1280-1 · Cited by 65
Nerve Repair Using Decellularized Nerve Grafts in Rat Models. A Review of the Literature
Lovati, A. B., D’Arrigo, D., Odella, S., Tos, P., Geuna, S., & Raimondo, S. (2018) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2018.00427 · Cited by 64
Performance and Morphology of Decellularized Pulmonary Valves Implanted in Juvenile Sheep
Quinn, R. W., Hilbert, S. L., Bert, A. A., Drake, B. W., Bustamante, J. A., Fenton, J., Moriarty, S. J., Neighbors, S., Lofland, G. K., & Hopkins, R. A. (2011) · The Annals of Thoracic Surgery · doi:10.1016/j.athoracsur.2011.03.039 · Cited by 62
Sun, W. Q., Xu, H. M., Sandor, M., & Lombardi, J. (2013) · Journal of Tissue Engineering · doi:10.1177/2041731413505305 · Cited by 62
Zhang, J., Wang, G. Y., Xiao, Y. P., Fan, L. Y., & Wang, Q. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.06.016 · Cited by 61
Metadata
- Authors
- Rieder, E., Kasimir, M.-T., Silberhumer, G., Seebacher, G., Wolner, E., Simon, P., & Weigel, G.
- Affiliations
- University of Vienna (AT)
- Corresponding author
- Weigel, Guenter
- Venue
- The Journal of Thoracic and Cardiovascular Surgery
- Publisher
- Elsevier BV
- Published
- 2004-02
- Volume
- 127
- Issue
- 2
- Pages
- 399-405
- ISSN
- 0022-5223 (ISSN-L)
- OpenAlex
- W2109385127
- Cited by
- 430
- References
- 29
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 21 · 2023: 21 · 2022: 30 · 2021: 23 · 2020: 25 · 2019: 25 · 2018: 17 · 2017: 28 · 2016: 22 · 2015: 22 · 2014: 26 · 2013: 24 · 2012: 16
- FWCI
- 11.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Journal of Thoracic and Cardiovascular Surgery (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- decellularization, porcine valves, recellularization, sodium deoxycholate, sodium dodecyl sulfate, trypsin, ribonuclease digestion, aortic valve, pulmonary valve, endothelial cells, myofibroblasts, detergent toxicity
- MeSH terms
- Aortic Valve, Biocompatible Materials, Bioprosthesis, Chelating Agents, Deoxycholic Acid, Deoxyribonucleases, Detergents, Edetic Acid, Endothelium, Vascular, Extracellular Matrix, Heart Valve Prosthesis, Humans, Microscopy, Electron, Prosthesis Design, Pulmonary Valve, Ribonucleases, Saphenous Vein, Sodium Dodecyl Sulfate, Surface-Active Agents, Octoxynol
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review