Cited by 64Effects of Environmental Stressors on LivestockAnimal health and immunologyThermoregulation and physiological responses

Expression Analysis of Toll like Receptors and Interleukins in Tharparkar Cattle during Acclimation to Heat Stress Exposure

Bharati, Jaya, Dangi, S.S., Mishra, S.R., Chouhan, V.S., Verma, V., Shankar, O., Bharti, M.K., Paul, A. +7 more·2017·Journal of Thermal Biology

Abstract

Six male Tharparkar cattle of 2-3 years old were selected for the study. After 15 days acclimation at thermo neutral zone (TNZ) in psychrometric chamber, animals were exposed at 42°C for 6h up to 23 days followed by 12 days of recovery period. Blood samples were collected during control period at TNZ (day 1, 5 and 12), after heat stress exposure (day 1-10, Short Term Heat Stress Acclimation - STHSA; day 15-23, Long Term Heat Stress Acclimation - LTHSA) and recovery period (day 7 and 12) and peripheral blood mononuclear cells (PBMCs) were isolated for RNA and protein extraction. Serum cortisol concentration was assessed by RIA. The mRNA and protein expression in PBMCs were determined by qPCR and western blot respectively. Samples at TNZ were taken as control. Serum cortisol concentration was increased (P<0.05) during STHSA and gradually declined during LTHSA. Toll like receptor 2 (TLR 2) expression was up regulated (P<0.05) during STHSA and declined to basal level during LTHSA and recovery phase. However, toll like receptor 4 (TLR 4) expression was up regulated (P<0.05) during STHSA and LTHSA while declined in recovery phase. Interleukin 2 (IL2) and interleukin 6 (IL 6) were up regulated (P<0.05) during STHSA and reduced to basal level during LTHSA. PBMCs culture study was conducted to study transcriptional abundance of TLR2/4 and IL2/6 at different temperature-time combinations. The present findings indicate that TLR 2/4 and IL 2/6 could possibly play a vital role in thermo tolerance in Tharparkar cattle during short term and long term heat stress exposure.

Integrity signals

Registry

Crossref · 10.1016/j.jtherbio.2017.02.002

Cites

54 of 54 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The expression of TLR2/4 and IL2/6 in blood was upregulated under both short-term and long-term heat stress.

    “Additionally, the expression of TLR2/4 and IL2/6 in blood was upregulated under both short-term and long-term heat stress [117].”

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Authors
Bharati, J., Dangi, S. S., Mishra, S. R., Chouhan, V. S., Verma, V., Shankar, O., Bharti, M. K., Paul, A., Mahato, D. K., Rajesh, G., Singh, G., Maurya, V. P., Bag, S., Kumar, P., & Sarkar, M.
Affiliations
Indian Veterinary Research Institute (IN)
Corresponding author
Sarkar, M.
Venue
Journal of Thermal Biology
Publisher
Elsevier BV
Published
2017-04
Volume
65
Pages
48-56
ISSN
0306-4565 (ISSN-L)
OpenAlex
W2586875384
Cited by
63
References
54
Citations by year
2026: 5 · 2025: 6 · 2024: 7 · 2023: 10 · 2022: 5 · 2021: 8 · 2020: 8 · 2019: 7 · 2018: 4 · 2017: 3
FWCI
6.70
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Thermal Biology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Initiative on Climate Resilient Agriculture (4-3/2010-IA-11)
  • Indian Veterinary Research Institute (IVRI/P&C/10-14/002)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Animal Science and Zoology › Effects of Environmental Stressors on Livestock
  • Health Sciences › Veterinary › Small Animals › Animal health and immunology
  • Health Sciences › Medicine › Physiology › Thermoregulation and physiological responses
Keywords
Tharparkar cattle, heat stress, TLR2, TLR4, interleukin-2, interleukin-6, peripheral blood mononuclear cells, serum cortisol, heat tolerance, gene expression, heat stress recovery
MeSH terms
Acclimatization, Animals, Cattle, Cells, Cultured, Gene Expression Regulation, Hot Temperature, Hydrocortisone, Interleukins, Male, Stress, Physiological, Toll-Like Receptors, Global Warming

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review