Cited by 98Mesenchymal stem cell researchBone Metabolism and DiseasesBone Tissue Engineering Materials

Effects of nicotine on proliferation and osteoblast differentiation in human alveolar bone marrow-derived mesenchymal stem cells

Kim, Beom-Su, Kim, Su-Jin, Kim, Hyung-Jin, Lee, Seung-Jin, Park, Yoon-Jeong, Lee, Jun, You, Hyung-Keun·2012·Life Sciences

Abstract

Aims Nicotine is a risk factor for various diseases, including osteoporosis, oral cancer, and periodontal disease. Numerous studies have elucidated the effects of nicotine on cell proliferation and differentiation. The purpose of this study was to determine the effects of nicotine on the proliferation and osteoblast differentiation of human alveolar bone marrow-derived mesenchymal stem cells (hABMMSCs).Main methods In this study, we treated hABMMSCs with different doses (1 μM to 5 mM) of nicotine. The survival and proliferation of hABMMSCs were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay and crystal violet assay. TUNEL and propidium iodide (PI) double staining assay were also performed. The effect of nicotine on osteoblast differentiation of hABMMSCs was determined by measuring calcium accumulation using alizarin red-sulfate (AR-S) staining, measurement of alkaline phosphatase (ALP) activity, and semi-quantitative PCR of osteoblast markers.Key findings The survival and proliferation of hABMMSCs did not differ when they were exposed to nicotine at concentrations ranging from 1 μM to 100 μM; however, cell proliferation increased when the cells were exposed to nicotine at concentrations of 1-2 mM and decreased significantly when exposed to 5mM of nicotine. A number of cells were stained by PI but not by TUNEL, and membrane vacuolization was observed in hABMMSCs treated with 5mM nicotine. Calcium accumulation; ALP activity; and mRNA levels of ALP, bone sialoprotein (BSP), collagen type I α 1 (Col1αI), and runt-related transcription factor 2 (Runx2) were significantly decreased by treatment with 2mM of nicotine, while osteocalcin transcripts decreased by treatment with 1 to 2 mM of nicotine.Significance These results suggest that nicotine has a bimodal effect on the proliferation and osteoblast differentiation in hABMMSCs.

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Crossref · 10.1016/j.lfs.2011.10.019

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  • Smoking exerts a direct effect on bone tissue via nicotinic acetylcholine receptors on osteoblasts, and nicotine's effect is dose-dependent: low concentrations stimulate osteoblast proliferation, whereas higher concentrations inhibit it.

    “Based on the findings of Kim and colleagues, the effect is dose-dependent—at low concentrations, nicotine appears to have a stimulating effect on osteoblast proliferation, whereas at higher concentrations, the effect is inhibitory [43].”

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Authors
Kim, B.-S., Kim, S.-J., Kim, H.-J., Lee, S.-J., Park, Y.-J., Lee, J., & You, H.-K.
Affiliations
  • Wonkwang University (KR)
  • Daegu Haany University (KR)
  • Ewha Womans University (KR)
  • Seoul National University (KR)
Corresponding author
  • Lee, Jun
  • You, Hyung-Keun
Venue
Life Sciences
Publisher
Elsevier BV
Published
2012-01
Volume
90
Issue
3-4
Pages
109-115
ISSN
0024-3205 (ISSN-L)
OpenAlex
W2029499588
Cited by
98
References
29
Citations by year
2026: 5 · 2025: 5 · 2024: 10 · 2023: 6 · 2022: 11 · 2021: 5 · 2020: 10 · 2019: 9 · 2018: 7 · 2017: 6 · 2016: 4 · 2015: 6 · 2014: 2 · 2013: 6 · 2012: 6
FWCI
2.26
Type
journal article
Language
en
Open access
closed
Locations
  • Life Sciences (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fonds National de la Recherche Luxembourg
  • National Research Foundation of Korea
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
  • Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
Keywords
nicotine, mesenchymal stem cells, osteogenic differentiation, cell proliferation, osteoporosis, alkaline phosphatase, Runx2, osteocalcin, bone sialoprotein, type I collagen, apoptosis, dose-dependent effects, periodontal disease
MeSH terms
Collagen Type I, alpha 1 Chain, Alveolar Process, Bone Marrow Cells, Cell Differentiation, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Nicotine, Osteoblasts, Cell Proliferation, Mesenchymal Stem Cells

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