An active lifestyle induces positive antioxidant enzyme modulation in peripheral blood mononuclear cells of overweight/obese postmenopausal women
Abstract
Aims The aim of this study was to investigate the effects of an active lifestyle on mitochondrial functioning, viability, bioenergetics, and redox status markers in peripheral blood mononuclear cells (PBMC) of overweight/ obese postmenopausal women.Materials and methods We performed a cross-sectional study with postmenopausal women aged 45–64 years and body mass index N 25 kg/m2, divided into physically active (n = 23) and sedentary (n = 12) groups. Mitochondria functioning and viability, bioenergetics and redox status parameters were assessed in PBMC with spectrophotometric and fluorometric assays.Key findings No differences were found in the enzyme activity of complexes I and II of the electron transport chain (ETC), mitochondrial superoxide dismutase (MnSOD) activity, methyl-tetrazolium reduction levels and reduced glutathione and oxidized glutathione levels between the groups. However, the physically active group presented higher levels of reactive oxygen species (ROS) (P= 0.04) and increased catalase (CAT) (P= 0.029), total (P= 0.011) and cytosolic SOD (CuZnSOD) (P= 0.009) activities.Significance An active lifestyle that includes aerobic exercise for at least 30 min, three times per week may improve antioxidant enzyme activities in PBMC in overweight/obese postmenopausal women, without changes in the activity of the ETC enzymes. However, this low intensity physical activity is not able to induce relevant mitochondrial adaptations.
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Exercise might reduce viral reactivation in lean, overweight and obese individuals by decreasing adiposity and maintaining redox balance, limiting accumulation of late-stage differentiated CMV-specific T cells.
“considering exercise decreases visceral and subcutaneous adiposity even in the absence of “weight loss” (Ross and Janiszewski 2008; Tchernof and Despres 2013), and exercise is a potent anti-inflammatory stimulus that helps maintain redox balance even in obesity (Farinha et al. 2015; Gleeson et al. 2011; Ji et al. 2006; Radak et al. 2008), then exercise might reduce viral reactivation in lean,”
From Is immunosenescence influenced by our lifetime “dose” of exercise? (2016) · cites this paper as Farinha et al. 2015
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Is immunosenescence influenced by our lifetime “dose” of exercise?
Turner, J. E. (2016) · Biogerontology · doi:10.1007/s10522-016-9642-z · Cited by 112 · see citing works
- Authors
- Farinha, J. B., De Carvalho, N. R., Steckling, F. M., De Vargas, L. D. S., Courtes, A. A., Stefanello, S. T., Martins, C. C., Bresciani, G., Dos Santos, D. L., & Soares, F. A. A.
- Affiliations
- Universidade Federal de Santa Maria (BR)
- Universidad Autónoma de Chile (CL)
- Corresponding author
- Farinha, Juliano Boufleur
- ORCID
- Venue
- Life Sciences
- Publisher
- Elsevier BV
- Published
- 2015-01
- Volume
- 121
- Pages
- 152-157
- ISSN
- 0024-3205 (ISSN-L)
- OpenAlex
- W2045009861
- Cited by
- 19
- References
- 42
- Citations by year
- 2026: 1 · 2025: 1 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 6 · 2019: 1 · 2018: 2 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Life Sciences (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Fundo de Amparo à Pesquisa do Estado do Rio Grande do Sul
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Health Sciences › Medicine › Physiology › Physical Activity and Health
- Keywords
- physical activity, sedentary lifestyle, postmenopausal women, overweight, obesity, peripheral blood mononuclear cells, antioxidant enzymes, reactive oxygen species, mitochondrial function, electron transport chain, superoxide dismutase, catalase, glutathione, mitochondrial bioenergetics, aerobic exercise
- MeSH terms
- Antioxidants, Catalase, Cross-Sectional Studies, Energy Metabolism, Female, Humans, Life Style, Middle Aged, Monocytes, Motor Activity, Obesity, Oxidation-Reduction, Superoxide Dismutase, Reactive Oxygen Species, Postmenopause, Overweight
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