Cited by 358Advanced Cellulose Research StudiesPolysaccharides and Plant Cell WallsNatural Fiber Reinforced Composites

The DOI matches its Crossref record; 51 of the 52 references it cites were found in a registry or an index; none of the 61 sources its claims cite is checked for support yet.

Cellulose nanofibers produced from banana peel by chemical and enzymatic treatment

Open access
Tibolla, Heloisa, Pelissari, Franciele Maria, Menegalli, Florencia Cecilia·2014·LWT - Food Science and Technology

Abstract

Cellulose nanofibers were isolated from banana peel bran using chemical treatment (CT—alkaline treatment, bleaching, and acid hydrolysis) and enzymatic treatment (ET—alkaline treatment and hydrolysis with xylanase). Both bran and cellulose nanofibers were characterized by size distribution, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR); the morphological features of the bran and the nanostructures were studied by scanning (SEM) and transmission electron microscopy (TEM), respectively. TEM analysis evidenced that both treatments effectively isolated banana fibers in the nanometer scale. Nanofibers originating from CT and ET had an average diameter of 10.9 and 7.6 nm and a length of 454.9 and 2889.7 nm, respectively. The aspect ratio of all the samples lay in the range of long fibers. In neutral water, the nanofiber suspensions exhibited high and negative zeta potential, which prevented the nanofibers from aggregating and yielded a more stable colloidal suspension. XRD studies revealed that CT afforded more crystalline cellulose nanofibers (58.6%) as compared with ET (49.2%). FTIR confirmed that the treatments removed most of the hemicelluloses and lignin present in the banana fibers. Nanoparticles isolated from the banana peel by both treatments have potential application as reinforcing elements in composites.

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TL;DR

Cellulose nanofibers were isolated from banana peel bran using chemical treatment (alkaline treatment, bleaching, acid hydrolysis) and enzymatic treatment (alkaline treatment, xylanase hydrolysis). Both treatments produced nanoscale fibers, but enzymatic treatment gave longer, higher-aspect-ratio, more stable suspensions, while chemical treatment gave more crystalline nanofibers.

Key findings

  • Both treatments effectively isolated banana fibers in the nanometer scale: CT nanofibers had average diameter 10.9 ± 2.3 nm, length 454.9 ± 6.6 nm, aspect ratio 42.7 ± 7.5, and zeta potential −16.1 ± 0.7 mV; ET nanofibers had diameter 7.6 ± 1.5 nm, length 2889.7 ± 214.3 nm, aspect ratio 353.9 ± 28.2, and zeta potential −25.7 ± 0.6 mV.
  • XRD crystallinity index was 15.0% for bran, 58.6% for CT-treated CNFs, and 49.2% for ET-treated CNFs; bran crystallinity improved by 300% after CT and 200% after ET.
  • ET furnished twice as high yield (10%) as CT (5.1%); nanofiber suspension concentrations were 0.54 and 0.57 g/100 g for CT and ET, respectively.
  • FTIR confirmed that treatments removed most hemicelluloses and lignin, but ET did not remove lignin completely, leaving residual lignin and bundles/impurities; CT was more aggressive and removed amorphous components more effectively.

Methods

  • Bran was prepared from the peel of unripe plantain bananas of the variety “Terra” (*Musa paradisiaca*) from southeastern Brazil, harvested in March 2013; peels were dried, milled, washed with ethanol, and sieved through a 200-mesh sieve.
  • CT used 5% w/v KOH, 1% w/v NaClO₂ bleaching at pH 5.0 and 70 °C, a second KOH treatment, and 1% v/v H₂SO₄ hydrolysis at 80 °C for 1 h; ET used 5% w/v KOH delignification followed by xylanase (50 U/g bran) in acetate buffer pH 5.0 at 45 °C for 24 h, then enzyme denaturation at 80 °C for 30 min.
  • Characterization included SEM, TEM, laser diffraction particle size, zeta potential, XRD crystallinity index, and FTIR; ANOVA and Tukey tests were run at 5% significance.

Limitations

  • The paper states that a future study will investigate the potential reinforcement that nanofibers from both treatments promote in composites; no composite mechanical testing was performed here.
  • The paper states that the samples were compared without the mechanical treatment step, even though a previous study found seven passages through a high-pressure homogenizer most suitable, because the objective was to isolate the effect of chemical versus enzymatic treatment.
  • The paper states that ET did not remove lignin completely and that ET nanofibers contained residual bundles/impurities; CT was more aggressive, produced shorter nanofibers, and had lower zeta potential, making those CNFs more likely to aggregate.

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Integrity signals

Registry

Crossref · 10.1016/j.lwt.2014.04.011

Cites

51 of 51 references verified0 citation errors · 0 not checkable

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Claims the paper makes

48 Not checked yet · 40 Own result · 23 Own interpretation · 10 No basis given · 1 Sources not found

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  • It may state two separate claims as one. 46
  • Probably not a substantial claim. 23
  • The quote only partly states this claim. 2
66 of 66
  • The paper relies on this without support.

    Enzymatic treatment allows for milder hydrolysis conditions compared with acid hydrolysis.

    “Enzymatic treatment has gained prominence and is developing rapidly: it allows for milder hydrolysis conditions as compared with acid hydrolysis.”
  • The paper relies on this without support.

    The omission of mechanical treatment does not prevent a valid comparison of the chemical and enzymatic treatments.

    “However, because the main objective of this study was to determine how the chemical and enzymatic treatments affected the CNFs isolation, we decided to compare the samples without the mechanical treatment step.”
  • The paper relies on this without support.

    The TEM images processed for diameter measurement are representative of the nanofiber population.

    “To determine the diameters of the fibers, the TEM images were processed using processing analysis software (ImageJ).”
  • The paper relies on this without support.

    The performance of cellulose nanofibers as reinforcing agents is associated with surface charge (zeta potential).

    “The performance of the nanofiber as reinforcing agent is also associated with other features such as surface charge (zeta potential).”
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Metadata

Authors
Tibolla, H., Pelissari, F. M., & Menegalli, F. C.
Affiliations
Universidade Estadual de Campinas (UNICAMP) (BR)
Corresponding author
Pelissari, Franciele Maria
Venue
LWT - Food Science and Technology
Publisher
Elsevier BV
Published
2014-12
Volume
59
Issue
2
Pages
1311-1318
ISSN
0023-6438 (ISSN-L)
OpenAlex
W1997398631
Cited by
358
References
52
Citations by year
2026: 19 · 2025: 27 · 2024: 36 · 2023: 34 · 2022: 44 · 2021: 39 · 2020: 42 · 2019: 38 · 2018: 28 · 2017: 24 · 2016: 17 · 2015: 10
FWCI
6.43
Type
journal article
Language
en
Open access
Locations
LWT (publishedVersion, cc-by-nc-nd)
Funders
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (2952/2011)
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (477842/2011-9)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Advanced Cellulose Research Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
  • Physical Sciences › Materials Science › Polymers and Plastics › Natural Fiber Reinforced Composites
Keywords
cellulose nanofibers, banana peel, alkali treatment, xylanase hydrolysis, acid hydrolysis, bleaching, hemicellulose removal, lignin removal, crystallinity, zeta potential, colloidal stability, nanofiber morphology, composite reinforcement

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