The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Evaluation of existing methods to extract microplastics from bivalve tissue: Adapted KOH digestion protocol improves filtration at single-digit pore size
Open accessAbstract
Methods standardisation in microplastics research is needed. Apart from reagent-dependent effects on microplastics, varying target particle sizes can hinder result comparison between studies. Human health concerns warrant recovery of small microplastics. We compared existing techniques using hydrogen peroxide, Proteinase-K, Trypsin and potassium hydroxide to digest bivalve tissue. Filterability, digestion efficacy, recoverability of microplastics and subsequent polymer identification using Raman spectroscopy and a matching software were assessed. Only KOH allowed filtration at ≤25 μm. When adding a neutralisation step prior to filtration, KOH digestates were filterable using 1.2-μm filters. Digestion efficacies were >95.0% for oysters, but lower for clams. KOH destroyed rayon at 60 °C but not at 40 °C. Acrylic fibre identification was affected due to changes in Raman spectra peaks. Despite those effects, we recommend KOH as the most viable extraction method for exposure risk studies, due to microplastics recovery from bivalve tissues of single-digit micrometre size. • Neutralising KOH prior to filtration allows for recovery of particles to 1.2 μm. • Polymer damage can be prevented by incubating samples at 40 °C instead of 60 °C. • Validation with an extended range of microplastics, including fibres and film. • Ultrapure water can effect Raman spectra. • Evaluation of Raman spectra of KOH-exposed microplastics with a matching software.
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Microplastics in the marine environment
Andrady, A. L. (2011) · Marine Pollution Bulletin · doi:10.1016/j.marpolbul.2011.05.030 · Cited by 8,105
Lost at sea: where is all the plastic?
Thompson, R. C., Olsen, Y., Mitchell, R. P., Davis, A., Rowland, S. J., John, A. W. G., McGonigle, D., & Russell, A. E. (2004) · Science · doi:10.1126/science.1094559 · Cited by 7,765
Self-citationHidalgo-Ruz, V., Gutow, L., Thompson, R. C., & Thiel, M. (2012) · Environmental Science & Technology · doi:10.1021/es2031505 · Cited by 5,440
The Physical Impacts of Microplastics on Marine Organisms: A Review
Wright, S. L., Thompson, R. C., & Galloway, T. S. (2013) · Environmental Pollution · doi:10.1016/j.envpol.2013.02.031 · Cited by 4,524
Plastic and Human Health: A Micro Issue?
Wright, S. L., & Kelly, F. J. (2017) · Environmental Science & Technology · doi:10.1021/acs.est.7b00423 · Cited by 3,413
Browne, M. A., Dissanayake, A., Galloway, T. S., Lowe, D. M., & Thompson, R. C. (2008) · Environmental Science & Technology · doi:10.1021/es800249a · Cited by 2,263
Microplastics in bivalves cultured for human consumption
Van Cauwenberghe, L., & Janssen, C. R. (2014) · Environmental Pollution · doi:10.1016/j.envpol.2014.06.010 · Cited by 2,074
Oyster reproduction is affected by exposure to polystyrene microplastics
Sussarellu, R., Suquet, M., Thomas, Y., Lambert, C., Fabioux, C., Pernet, M. E. J., Le Goïc, N., Quillien, V., Mingant, C., Epelboin, Y., et al. (2016) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1519019113 · Cited by 1,827
Rochman, C. M., Tahir, A., Williams, S. L., Baxa, D. V., Lam, R., Miller, J. T., Teh, F.-C., Werorilangi, S., & Teh, S. J. (2015) · Scientific Reports · doi:10.1038/srep14340 · Cited by 1,504
Microplastic Pollution in Table Salts from China
Yang, D., Shi, H., Li, L., Li, J., Jabeen, K., & Kolandhasamy, P. (2015) · Environmental Science & Technology · doi:10.1021/acs.est.5b03163 · Cited by 1,112
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Analysis of environmental nanoplastics: Progress and challenges
Cai, H., Xu, E. G., Du, F., Li, R., Liu, J., & Shi, H. (2021) · Chemical Engineering Journal · doi:10.1016/j.cej.2020.128208 · Cited by 444
Microplastics in Food: A Review on Analytical Methods and Challenges
Kwon, J.-H., Kim, J.-W., Pham, T. D., Tarafdar, A., Hong, S., Chun, S.-H., Lee, S.-H., Kang, D.-Y., Kim, J.-Y., Kim, S.-B., et al. (2020) · International Journal of Environmental Research and Public Health · doi:10.3390/ijerph17186710 · Cited by 308
Microplastics in fish and fishmeal: an emerging environmental challenge?
Thiele, C. J., Hudson, M. D., Russell, A. E., Saluveer, M., & Sidaoui-Haddad, G. (2021) · Scientific Reports · doi:10.1038/s41598-021-81499-8 · Cited by 298
Self-citationMicroplastic accumulation in the gastrointestinal tracts in birds of prey in central Florida, USA
Carlin, J., Craig, C., Little, S., Donnelly, M., Fox, D., Zhai, L., & Walters, L. (2020) · Environmental Pollution · doi:10.1016/j.envpol.2020.114633 · Cited by 292
Metadata
- Authors
- Thiele, C. J., Hudson, M. D., & Russell, A. E.
- Venue
- Marine Pollution Bulletin
- Publisher
- Elsevier BV
- Published
- 2019-05
- Volume
- 142
- Pages
- 384-393
- ISSN
- 0025-326X (ISSN-L)
- OpenAlex
- W2926505775
- Cited by
- 299
- References
- 49
- FWCI
- 9.48
- Type
- journal article
- Language
- en
- Open access
- hybrid
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