The DOI matches its Crossref record; 49 of the 50 references it cites were found in a registry or an index.
Inhalable dry powder mRNA vaccines based on extracellular vesicles
Open accessAbstract
Respiratory diseases are a global burden, with millions of deaths attributed to pulmonary illnesses and dysfunctions. Therapeutics have been developed, but they present major limitations regarding pulmonary bioavailability and product stability. To circumvent such limitations, we developed room-temperature-stable inhalable lung-derived extracellular vesicles or exosomes (Lung-Exos) as mRNA and protein drug carriers. Compared with standard synthetic nanoparticle liposomes (Lipos), Lung-Exos exhibited superior distribution to the bronchioles and parenchyma and are deliverable to the lungs of rodents and nonhuman primates (NHPs) by dry powder inhalation. In a vaccine application, severe acute respiratory coronavirus 2 (SARS-CoV-2) spike (S) protein encoding mRNA-loaded Lung-Exos (S-Exos) elicited greater immunoglobulin G (IgG) and secretory IgA (SIgA) responses than its loaded liposome (S-Lipo) counterpart. Importantly, S-Exos remained functional at room-temperature storage for one month. Our results suggest that extracellular vesicles can serve as an inhaled mRNA drug-delivery system that is superior to synthetic liposomes.
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“One of the key benefits of exosomes is their ability to express surface proteins and receptors of their donor cells, allowing them to target recipient cells within the same tissue effectively. For example, lung spheroid cell-derived exosomes can deliver vaccines directly to lung tissue [82].”
From Exosome for mRNA delivery: Strategies and therapeutic applications (2024) · cites this paper as [82]
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Cites
Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine
Polack, F. P., Thomas, S. J., Kitchin, N., Absalon, J., Gurtman, A., Lockhart, S., Perez, J. L., Pérez Marc, G., Moreira, E. D., Zerbini, C., et al. (2020) · New England Journal of Medicine · doi:10.1056/nejmoa2034577 · Cited by 14,592
Shedding light on the cell biology of extracellular vesicles
van Niel, G., D'Angelo, G., & Raposo, G. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2017.125 · Cited by 8,369
A novel coronavirus outbreak of global health concern
Wang, C., Horby, P. W., Hayden, F. G., & Gao, G. F. (2020) · The Lancet · doi:10.1016/s0140-6736(20)30185-9 · Cited by 7,700
Exosomes: composition, biogenesis and function
Théry, C., Zitvogel, L., & Amigorena, S. (2002) · Nature reviews. Immunology · doi:10.1038/nri855 · Cited by 5,478
RNA-Based COVID-19 Vaccine BNT162b2 Selected for a Pivotal Efficacy Study
Walsh, E. E., Frenck, R., Falsey, A. R., Kitchin, N., Absalon, J., Gurtman, A., Lockhart, S., Neuzil, K., Mulligan, M. J., Bailey, R., et al. (2020) · doi:10.1101/2020.08.17.20176651 · Cited by 2,615
SARS-CoV-2 mRNA Vaccine Design Enabled by Prototype Pathogen Preparedness
Corbett, K. S., Edwards, D. K., Leist, S. R., Abiona, O. M., Boyoglu-Barnum, S., Gillespie, R. A., Himansu, S., Schäfer, A., Ziwawo, C. T., DiPiazza, A. T., et al. (2020) · Nature · doi:10.1038/s41586-020-2622-0 · Cited by 1,615
Freeze-drying of nanoparticles: Formulation, process and storage considerations
ABDELWAHED, W., DEGOBERT, G., STAINMESSE, S., & FESSI, H. (2006) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2006.09.017 · Cited by 1,611
Extracellular Vesicles: Unique Intercellular Delivery Vehicles
Maas, S. L. N., Breakefield, X. O., & Weaver, A. M. (2016) · Trends in Cell Biology · doi:10.1016/j.tcb.2016.11.003 · Cited by 1,466
A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity
Yang, H. J., Wang, W., Chen, Z., Lu, S., Yang, F., Bi, Z., Bao, L., Mo, F., Li, X., Huang, Y., et al. (2020) · Nature · doi:10.1038/s41586-020-2599-8 · Cited by 767
Self-citationExosomes Derived from Bone Marrow Mesenchymal Stem Cells as Treatment for Severe COVID-19
Sengupta, V., Sengupta, S., Lazo, A., Woods, P., Nolan, A., & Bremer, N. J. (2020) · Stem Cells and Development · doi:10.1089/scd.2020.0080 · Cited by 751
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Huang, Y., Guo, X., Wu, Y., Chen, X., Feng, L., Xie, N., & Shen, G. (2024) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-024-01745-z · Cited by 537
The landscape of mRNA nanomedicine
Huang, X., Kong, N., Zhang, X., Cao, Y., Langer, R., & Tao, W. (2022) · Nature Medicine · doi:10.1038/s41591-022-02061-1 · Cited by 511
Recent advances in extracellular vesicles for therapeutic cargo delivery
Kim, H. I., Park, J., Zhu, Y., Wang, X., Han, Y., & Zhang, D. (2024) · Experimental & Molecular Medicine · doi:10.1038/s12276-024-01201-6 · Cited by 505
Liu, M., Hu, S., Yan, N., Popowski, K. D., & Cheng, K. (2024) · Nature Nanotechnology · doi:10.1038/s41565-023-01580-3 · Cited by 277
Self-citationCheng, K., & Kalluri, R. (2023) · Extracellular Vesicle · doi:10.1016/j.vesic.2023.100029 · Cited by 231
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Metadata
- Authors
- Popowski, K. D., Moatti, A., Scull, G., Silkstone, D., Lutz, H., López de Juan Abad, B., George, A., Belcher, E., Zhu, D., Mei, X., Cheng, X., Cislo, M., Ghodsi, A., Cai, Y., Huang, K., Li, J., Brown, A. C., Greenbaum, A., Dinh, P.-U. C., & Cheng, K.
- Affiliations
- North Carolina State University (US)
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Dinh, Phuong-Uyen C.
- Cheng, Ke
- ORCID
- Venue
- Matter
- Publisher
- Elsevier BV
- Published
- 2022-09
- Volume
- 5
- Issue
- 9
- Pages
- 2960-2974
- ISSN
- 2590-2385 (ISSN-L)
- PMC
- PMC9272513
- OpenAlex
- W4285008938
- Cited by
- 218
- References
- 50
- Citations by year
- 2026: 33 · 2025: 62 · 2024: 59 · 2023: 55 · 2022: 8
- FWCI
- 15.02
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 371 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Matter (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (HL164998, HL137093, HL144002, HL149940, HL146701, HL146153, HL147357, HL123920)
- American Heart Association (19EIA34660286, T32GM133393, 18TPA34230092)
- College of Veterinary Medicine, North Carolina State University
- North Carolina State University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Inhalation and Respiratory Drug Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- extracellular vesicles, exosomes, mRNA vaccines, dry powder inhalation, pulmonary drug delivery, liposomes, SARS-CoV-2 spike protein, secretory IgA, IgG antibody response, room-temperature stability, non-human primates
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