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Cited by 205Nitric Oxide and Endothelin EffectsMetabolism, Diabetes, and CancerDiabetes Treatment and Management

Metformin Decreases Intracellular Production of Reactive Oxygen Species in Aortic Endothelial Cells

Ouslimani, Nadjat, Peynet, Jacqueline, Bonnefont-Rousselot, Dominique, Thérond, Patrice, Legrand, Alain, Beaudeux, Jean-Louis·2005·Metabolism
DOI 10.1016/j.metabol.2005.01.029
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Abstract

Beyond its antidiabetic activity justifying its use in the treatment of the type 2 diabetes, metformin (MET [dimethylguanidine, Glucophage]) has been shown to exhibit antioxidant properties in vitro, which could contribute to limit the deleterious vascular complications of diabetes. We investigated whether MET, at the pharmacological level of 10 -5 mol/L, was able to modulate intracellular production of reactive oxygen species (ROS) both in quiescent bovine aortic endothelial cells (BAECs) and in BAECs stimulated by a short incubation with high levels of glucose (30 mmol/L, 2 hours) or angiotensin II (10 -7 mol/L, 1 hour). Intracellular ROS production was measured by fluorescence of the DCF (2,7-dichlorodihydrofluorescein) probe. Our results showed that MET was able to reduce the intracellular production of ROS in both nonstimulated BAECs (-20%, P < .05) and BAEC stimulated by high levels of glucose or angiotensin II (-28% and -72%, respectively, P < .01). Experiments performed in the presence of the nicotinamide adenine dinucleotide phosphate [NAD(P)H] oxidase inhibitor apocynin or the respiratory mitochondrial chain inhibitor rotenone indicated that MET exerted its effect partly through an inhibition of the formation of ROS produced mainly by NAD(P)H oxidase and also, to a lesser extent, by the respiratory mitochondrial chain.

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Crossref · 10.1016/j.metabol.2005.01.029

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  • Metformin and other drugs used for T2DM, like SGLT2 inhibitors, may also reduce hyperglycemia-induced elevated endothelial ROS, independent of negative effects on complex 1, such as via inhibition of NADPH oxidase or blocking entry of glucose into the endothelium.

    “Metformin and other drugs used for T2DM, like the SGLT2 inhibitors, may also reduce hyperglycemia-induced elevated endothelial ROS, independent of negative effects on complex 1, such as via inhibition of NADPH oxidase or blocking the entry of glucose into the endothelium (32, 107–109, 187, 188)”

    From A Critical Review of the Evidence That Metformin Is a Putative Anti-Aging Drug That Enhances Healthspan and Extends Lifespan (2021) · cites this paper as [107]

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Authors
Ouslimani, N., Peynet, J., Bonnefont-Rousselot, D., Thérond, P., Legrand, A., & Beaudeux, J.-L.
Corresponding author
Beaudeux, Jean-Louis
ORCID
Venue
Metabolism
Publisher
Elsevier BV
Published
2005-06
Volume
54
Issue
6
Pages
829-834
ISSN
0026-0495 (ISSN-L)
DOI
10.1016/j.metabol.2005.01.029
OpenAlex
W2103676852
Cited by
204
References
54
Citations by year
2026: 5 · 2025: 5 · 2024: 8 · 2023: 5 · 2022: 6 · 2021: 20 · 2020: 14 · 2019: 10 · 2018: 9 · 2017: 9 · 2016: 15 · 2015: 13 · 2014: 11 · 2013: 12 · 2012: 11
FWCI
4.60
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Metabolism (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes Treatment and Management
Keywords
metformin, reactive oxygen species, aortic endothelial cells, antioxidant activity, NADPH oxidase, apocynin, mitochondrial respiratory chain, angiotensin II, high glucose, diabetic vascular complications, type 2 diabetes
MeSH terms
Animals, Aorta, Cattle, Cells, Cultured, Hypoglycemic Agents, Metformin, Reactive Oxygen Species, NADPH Oxidases, Endothelial Cells

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