CoChat
Sign Up
Cited by 19Mitochondrial Function and PathologyAdipose Tissue and MetabolismMetabolism, Diabetes, and Cancer

Short-term increase of plasma free fatty acids does not interfere with intrinsic mitochondrial function in healthy young men

Brands, Myrte, Hoeks, Joris, Sauerwein, Hans P., Ackermans, Mariette T., Ouwens, Margriet, Lammers, Nicolette M., van der Plas, Mart N., Schrauwen, Patrick +2 more·2011·Metabolism
DOI 10.1016/j.metabol.2011.02.006
Open this paper in CoChat — free account

Abstract

Free fatty acid (FFA)- and obesity-induced insulin resistance has been associated with disturbed mitochondrial function. Elevated plasma FFA can impair insulin-induced increase of adenosine triphosphate synthesis and downregulate the expression of genes important in the biogenesis of mitochondria in human skeletal muscle. Whether FAs have a direct effect on intrinsic mitochondrial capacity remains to be established. Therefore, we measured ex vivo mitochondrial respiratory capacity in human skeletal muscle after exposure to hyperinsulinemia and high levels of plasma FFA. Nine healthy lean men were studied during a 6-hour hyperinsulinemic (600 pmol/L) euglycemic clamp with concomitant infusion of Intralipid (Fresensius Kabi Nederland, Den Bosch, the Netherlands) (FFA clamped at 0.5 mmol/L) or saline. Mitochondrial respiratory capacity was measured by high-resolution respirometry in permeabilized muscle fibers using an Oxygraph (OROBOROS Instruments, Innsbruck, Austria). Each participant served as his own control. Peripheral glucose uptake (rate of disappearance) was significantly lower during infusion of the lipid emulsion compared with the control saline infusion (68 μmol/kg·min [saline] vs 40 μmol/kg·min [lipid], P = .008). However, adenosine diphosphate-stimulated and maximal carbonylcyanide-4-(trifluoromethoxy)-phenylhydrazone-stimulated uncoupled respiration rates were not different in permeabilized skeletal muscle fibers after exposure to high levels of FFA compared with the control condition. We conclude that short-term elevation of FFA within the physiological range induces insulin resistance but does not affect intrinsic mitochondrial capacity in skeletal muscle in humans.

Integrity signals

Registry

Crossref · 10.1016/j.metabol.2011.02.006

Cites

48 of 48 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Short-term lipid infusion for 6 to 8 h in healthy individuals does not change mitochondrial content, morphology, and respiration rates in skeletal muscle despite a lower mitochondrial membrane potential.

    “Short-term lipid infusion for 6 to 8 h in healthy individuals does not change mitochondrial content, morphology, and respiration rates in skeletal muscle despite a lower mitochondrial membrane potential (4, 10).”

    From Mitochondrial Fission Contributes to Mitochondrial Dysfunction and Insulin Resistance in Skeletal Muscle (2012) · cites this paper as [4]

Cites

Flagged first, then by citations

1 of the 49 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Brands, M., Hoeks, J., Sauerwein, H. P., Ackermans, M. T., Ouwens, M., Lammers, N. M., van der Plas, M. N., Schrauwen, P., Groen, A. K., & Serlie, M. J.
Affiliations
  • Amsterdam UMC Location University of Amsterdam (NL)
  • Maastricht University Medical Centre (NL)
Corresponding author
Brands, Myrte
ORCID
Venue
Metabolism
Publisher
Elsevier BV
Published
2011-10
Volume
60
Issue
10
Pages
1398-1405
ISSN
0026-0495 (ISSN-L)
DOI
10.1016/j.metabol.2011.02.006
OpenAlex
W2162493960
Cited by
19
References
49
Citations by year
2024: 3 · 2018: 1 · 2016: 1 · 2015: 4 · 2014: 2 · 2013: 4 · 2012: 1
FWCI
1.30
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Metabolism (publishedVersion)
  • PubMed (publishedVersion)
  • Research Publications (Maastricht University) (publishedVersion)
  • Pure Amsterdam UMC (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Research Publications (Maastricht University) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
Keywords
plasma free fatty acids, insulin resistance, mitochondrial respiratory capacity, skeletal muscle, hyperinsulinemic-euglycemic clamp, Intralipid, lipid emulsions, glucose uptake, mitochondrial biogenesis, uncoupled respiration, ATP production
MeSH terms
Adult, Biopsy, Blood Glucose, Fatty Acids, Nonesterified, Health, Humans, Insulin, Insulin Resistance, Male, Mitochondria, Muscle, Oxidation-Reduction, Time Factors, Glucose Clamp Technique, Muscle, Skeletal, Cell Respiration, Young Adult

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review