l-Arginine enriched biscuits improve endothelial function and glucose metabolism: A pilot study in healthy subjects and a cross-over study in subjects with impaired glucose tolerance and metabolic syndrome

Monti, Lucilla D.; Casiraghi, Maria C.; Setola, Emanuela; Galluccio, Elena; Pagani, Maria A.; Quaglia, Lucio; Bosi, Emanuele; Piatti, PierMarco

doi:10.1016/j.metabol.2012.08.004

Cited by 62Nitric Oxide and Endothelin EffectsDiabetes, Cardiovascular Risks, and LipoproteinsDiet, Metabolism, and Disease

l-Arginine enriched biscuits improve endothelial function and glucose metabolism: A pilot study in healthy subjects and a cross-over study in subjects with impaired glucose tolerance and metabolic syndrome

Monti, Lucilla D., Casiraghi, Maria C., Setola, Emanuela, Galluccio, Elena, Pagani, Maria A., Quaglia, Lucio, Bosi, Emanuele, Piatti, PierMarco·2013·Metabolism

DOI 10.1016/j.metabol.2012.08.004

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Abstract

Objective The aim of this study was to evaluate the effects of a new L-arginine-enriched biscuit on endothelial function, insulin sensitivity/secretion and body composition.Materials/methods The project was composed of two studies. The first study was an acute pilot postprandial study in 7 healthy subjects that evaluated bio-availability and vascular effects of L-arginine-enriched biscuits that contained 6.6 gL-arginine, 21.9 g carbohydrates, 3.6 g protein, 7.5 g fat and 4.3 g dietary fiber compared with placebo biscuits and 6.6 g powdered L-arginine. Subjects underwent the tests in random order, in at least 14-day intervals. The second study was a double-blind crossover study in 15 obese subjects with IGT and MS. These subjects consumed 6.6 g of L-arginine-enriched biscuits or placebo biscuits in a 1600 kcal diet. Each study period lasted 2 weeks with a 2-week washout in between. Endothelial function, glucose tolerance, insulin sensitivity and insulin secretion were evaluated at the end of each intervention period.Results In the first study, the groups that received the L-arginine-enriched biscuits and the powdered L-arginine had similarly increased L-arginine, NOx and cGMP levels and post-ischemic blood flow (PI-BF). In both cases, these levels were significantly higher than those in the placebo biscuit recipient group. In the second study, the L-arginine-enriched biscuit recipient group displayed increased L-arginine, NOx, cGMP, PI-BF, and Matsuda index levels, whereas their circulating glucose, proinsulin/insulin ratio and fat mass were decreased compared with the placebo biscuit recipient group.Conclusions L-Arginine-enriched biscuits with low sugar and protein content enhance endothelial function and improve glucose metabolism, insulin sensitivity and insulin secretion in subjects with IGT and MS.

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The effects of NO in the vasculature at nM/pM concentrations are mainly mediated through activation of the canonical NO receptor sGC.
“The effects of NO in the vasculature at concentrations in the nM/pM range (397) are mainly mediated through the activation of the canonical NO receptor sGC (223, 662, 711, 751).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [662]
Human studies have repeatedly shown that L-arginine supplementation has favorable effects on body composition and insulin sensitivity.
“However, human studies have repeatedly shown that L-arginine supplementation has favorable effects on body composition and insulin sensitivity [271-277].”
From Regulation of obesity and insulin resistance by nitric oxide (2014) · cites this paper as [277]

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Authors: Monti, L. D., Casiraghi, M. C., Setola, E., Galluccio, E., Pagani, M. A., Quaglia, L., Bosi, E., & Piatti, P.
Affiliations: IRCCS Ospedale San Raffaele (IT)
University of Milan (IT)
Pavia nel Cuore (IT)
Corresponding author: Monti, Lucilla D.
ORCID: Monti, Lucilla D.
Casiraghi, Maria C.
Pagani, Maria A.
Bosi, Emanuele
Venue: Metabolism
Publisher: Elsevier BV
Published: 2013-02
Volume: 62
Issue: 2
Pages: 255-264
ISSN: 0026-0495 (ISSN-L)
DOI: 10.1016/j.metabol.2012.08.004
OpenAlex: W2154202146
Cited by: 60
References: 37
Citations by year: 2026: 2 · 2025: 2 · 2024: 1 · 2023: 5 · 2022: 8 · 2021: 6 · 2020: 1 · 2019: 7 · 2018: 4 · 2017: 3 · 2016: 8 · 2015: 2 · 2014: 6 · 2013: 5
FWCI: 2.79
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
Locations: Metabolism (publishedVersion)
PubMed (publishedVersion)
Archivio Istituzionale della Ricerca - Università Vita-Salute San Raffaele (submittedVersion)
Topics: Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diabetes, Cardiovascular Risks, and Lipoproteins
Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
Keywords: L-arginine, endothelial function, glucose metabolism, insulin sensitivity, insulin secretion, impaired glucose tolerance, metabolic syndrome, obesity, nitric oxide, cyclic GMP, Matsuda index, proinsulin/insulin ratio, fat mass, bioavailability, dietary supplementation
MeSH terms: Insulin Secretion, Arginine, Body Composition, Body Weight, Cholesterol, Double-Blind Method, Endothelium, Vascular, Female, Cyclic GMP, Humans, Insulin, Male, Middle Aged, Obesity, Pilot Projects, Triglycerides, Glucose Intolerance, Cross-Over Studies, Metabolic Syndrome, Nitric Oxide Synthase Type II, Snacks

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l-Arginine enriched biscuits improve endothelial function and glucose metabolism: A pilot study in healthy subjects and a cross-over study in subjects with impaired glucose tolerance and metabolic syndrome

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