Cited by 624Probiotics and Fermented FoodsBacterial Identification and Susceptibility TestingMicrobial Community Ecology and Physiology

PCR-DGGE fingerprinting: novel strategies for detection of microbes in food

Ercolini, Danilo·2004·Journal of Microbiological Methods

Abstract

Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isolated from food, the evaluation of microbial diversity during food fermentation, and microbiological and commercial food quality assessment. Potentials and limitations of this culture-independent approach in food microbiology are indicated and future perspectives are discussed.

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Crossref · 10.1016/j.mimet.2003.11.006

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Authors
Ercolini, D.
Affiliations
University of Naples Federico II (IT)
Corresponding author
Ercolini, Danilo
Venue
Journal of Microbiological Methods
Publisher
Elsevier BV
Published
2004-03
Volume
56
Issue
3
Pages
297-314
ISSN
0167-7012 (ISSN-L)
OpenAlex
W2078886175
Cited by
624
References
83
Citations by year
2026: 5 · 2025: 8 · 2024: 10 · 2023: 11 · 2022: 21 · 2021: 16 · 2020: 15 · 2019: 24 · 2018: 23 · 2017: 22 · 2016: 39 · 2015: 48 · 2014: 37 · 2013: 42 · 2012: 41
FWCI
1.96
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Microbiological Methods (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministero dell’Istruzione, dell’Università e della Ricerca
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Keywords
microbial community analysis, food microbiology, culture-independent detection, microbial diversity, food fermentation, food quality assessment, food safety, microbial identification
MeSH terms
Bacteria, Electrophoresis, Polyacrylamide Gel, Fermentation, Food Microbiology, Fungi, Nucleic Acid Denaturation, Quality Control, Polymerase Chain Reaction, DNA Fingerprinting, Ecosystem

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