PCR-DGGE fingerprinting: novel strategies for detection of microbes in food
Abstract
Polymerase chain reaction denaturing gradient gel electrophoresis (PCR-DGGE) fingerprinting was recently introduced into food microbiology. This paper describes the technique and reports on the state-of-the-art application of this technique to food and food-related ecosystems. Applications of PCR-DGGE in several fields of food microbiology are reviewed: the identification of microorganisms isolated from food, the evaluation of microbial diversity during food fermentation, and microbiological and commercial food quality assessment. Potentials and limitations of this culture-independent approach in food microbiology are indicated and future perspectives are discussed.
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- Authors
- Ercolini, D.
- Affiliations
- University of Naples Federico II (IT)
- Corresponding author
- Ercolini, Danilo
- ORCID
- Ercolini, Danilo
- Venue
- Journal of Microbiological Methods
- Publisher
- Elsevier BV
- Published
- 2004-03
- Volume
- 56
- Issue
- 3
- Pages
- 297-314
- ISSN
- 0167-7012 (ISSN-L)
- OpenAlex
- W2078886175
- Cited by
- 624
- References
- 83
- Citations by year
- 2026: 5 · 2025: 8 · 2024: 10 · 2023: 11 · 2022: 21 · 2021: 16 · 2020: 15 · 2019: 24 · 2018: 23 · 2017: 22 · 2016: 39 · 2015: 48 · 2014: 37 · 2013: 42 · 2012: 41
- FWCI
- 1.96
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Microbiological Methods (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministero dell’Istruzione, dell’Università e della Ricerca
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Bacterial Identification and Susceptibility Testing
- Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
- Keywords
- microbial community analysis, food microbiology, culture-independent detection, microbial diversity, food fermentation, food quality assessment, food safety, microbial identification
- MeSH terms
- Bacteria, Electrophoresis, Polyacrylamide Gel, Fermentation, Food Microbiology, Fungi, Nucleic Acid Denaturation, Quality Control, Polymerase Chain Reaction, DNA Fingerprinting, Ecosystem
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