Cited by 87Yeasts and Rust Fungi StudiesProbiotics and Fermented FoodsFermentation and Sensory Analysis

Identification of Yeasts and Evaluation of their Distribution in Taiwanese Kefir and Viili Starters

Open access
Wang, S.Y., Chen, H.C., Liu, J.R., Lin, Y.C., Chen, M.J.·2008·Journal of Dairy Science

Abstract

The objective of the present study was to investigate yeast communities in kefir grains and viili starters in Taiwan through conventional microbiological cultivation and polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The DNA sequencing was used as a validity technique to ensure that all isolates within each group belonged to just one species, and to confirm the identified results of PCR-DGGE. Results indicated that a combination of conventional microbiological cultivation with PCR-DGGE and sequencing could successfully identify 4 yeast species from both types of cultures in Taiwan. Kluyveromyces marxianus, Saccharomyces turicensis, and Pichia fermentans were found in Taiwanese kefir grains with a distribution of 76, 22, and 2%, respectively, whereas Klu. marxianus, Saccharomyces unisporus and P. fermentans were identified in viili starters corresponding to 58, 11, and 31% of the total cell counts, respectively. Furthermore, the culture-independent method was applied to identify the yeast species using DGGE. Only 2 yeast species, Klu. marxianus and S. turicensis, were found in kefir grains and 2, Klu. marxianus and P. fermentans, in viili starters. These results suggest that in samples containing multiple species, PCR-DGGE may fail to detect some species. Sequences of yeast isolates reported in this study have been deposited in the GenBank database under accession nos. DQ139802, AF398485, DQ377652, and AY007920.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.3168/jds.2007-0468

Cites

49 of 49 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 50 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Wang, S. Y., Chen, H. C., Liu, J. R., Lin, Y. C., & Chen, M. J.
Affiliations
National Taiwan University (TW)
Corresponding author
Chen, M.J.
Venue
Journal of Dairy Science
Published
2008-10
Volume
91
Issue
10
Pages
3798-3805
ISSN
0022-0302 (ISSN-L)
OpenAlex
W2050026551
Cited by
87
References
50
Citations by year
2026: 4 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 5 · 2021: 5 · 2020: 7 · 2019: 8 · 2018: 2 · 2017: 7 · 2016: 8 · 2015: 2 · 2014: 3 · 2013: 7 · 2012: 6
FWCI
2.19
Type
journal article
Language
en
Open access
Locations
  • Journal of Dairy Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Food Industry Research and Development Institute
  • National Science Council
  • Beef Cattle Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Fermentation and Sensory Analysis
Keywords
kefir grains, yeast communities, Kluyveromyces marxianus, Pichia fermentans, yeast identification, PCR-DGGE, culture-independent detection
MeSH terms
Food Microbiology, Microbiological Techniques, Molecular Sequence Data, Taiwan, Yeasts, Polymerase Chain Reaction, Cultured Milk Products

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review