An EcR homolog from the filarial parasite, Dirofilaria immitis requires a ligand-activated partner for transactivation
Abstract
Filarial parasites are responsible for several serious human diseases with symptoms such as lymphoedema, elephantiasis, and blindness. An understanding of how these parasites pass through developmental checkpoints may suggest potential targets for intervention. A useful model system for the study of the human parasites is the closely related nematode, D. immitis, the causative agent of dog heartworm disease. Ecdysteroids have been identified in filarial nematodes and have been shown to have a biological affect both on molting and microfilarial production. The ecdysteroid, 20-hydroxyecdysone and its receptor, EcR, have a well-characterized developmental role in insects, where it is involved in the control of molting and metamorphosis. We have identified a D. immitis nuclear receptor, DiEcR that shows strong sequence similarity to the insect EcR and shares many of its biochemical properties, including ligand-dependent activation of transcription. However, unlike most insect EcRs, DiEcR requires a ligand-activated RXR partner to exhibit ligand-dependent transcriptional activation of a reporter gene in tissue culture.
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A receptor for 20-hydroxyecdisone (DiEcR) sharing multiple biochemical features with a similar receptor in insects has been identified in D. immitis and characterized.
“A receptor for 20-hydroxyecdisone (DiEcR) that shares multiple biochemical features with a similar receptor in insects has been identified in D. immitis and characterized.”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [386]
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- Authors
- Shea, C., Richer, J., Tzertzinis, G., & Maina, C. V.
- Affiliations
- New England Biolabs (United States) (US)
- University of Colorado Anschutz (US)
- Corresponding author
- Maina, Claude V.
- Venue
- Molecular and Biochemical Parasitology
- Publisher
- Elsevier BV
- Published
- 2010-06
- Volume
- 171
- Issue
- 2
- Pages
- 55-63
- ISSN
- 0166-6851 (ISSN-L)
- OpenAlex
- W2021822587
- Cited by
- 21
- References
- 49
- Citations by year
- 2024: 2 · 2022: 2 · 2021: 2 · 2020: 1 · 2017: 1 · 2014: 1 · 2013: 2 · 2012: 3
- FWCI
- 2.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Molecular and Biochemical Parasitology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Keywords
- Dirofilaria immitis, ecdysone receptor, nuclear receptors, retinoid X receptor, ecdysteroids, filarial nematodes, developmental regulation, molting
- MeSH terms
- Amino Acid Sequence, Animals, Dirofilaria immitis, Gene Expression Regulation, Humans, Ligands, Molecular Sequence Data, Protein Binding, Receptors, Steroid, Transcriptional Activation, Sequence Alignment, Sequence Analysis, DNA, Genes, Reporter, DNA, Helminth, Electrophoretic Mobility Shift Assay, NIH 3T3 Cells, Retinoid X Receptors, Mice
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