Drosophila Ecdysone Receptor Mutations Reveal Functional Differences among Receptor Isoforms
Open accessAbstract
The steroid hormone ecdysone directs Drosophila metamorphosis via three heterodimeric receptors that differ according to which of three ecdysone receptor isoforms encoded by the EcR gene (EcR-A, EcR-B1, or EcR-B2) is activated by the orphan nuclear receptor USP. We have identified and molecularly mapped two classes of EcR mutations: those specific to EcR-B1 that uncouple metamorphosis, and embryonic-lethal mutations that map to common sequences encoding the DNA- and ligand-binding domains. In the larval salivary gland, loss of EcR-B1 results in loss of activation of ecdysone-induced genes. Comparable transgenic expression of EcR-B1, EcR-B2, and EcR-A in these mutant glands results, respectively, in full, partial, and no repair of that loss.
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20E initiates the genomic pathway by binding with its nuclear hormone receptor EcR to regulate gene expression for metamorphosis.
“20E initiates the genomic pathway by binding with its nuclear hormone receptor EcR to regulate gene expression for metamorphosis [37].”
From G-protein-coupled receptor participates in 20-hydroxyecdysone signaling on the plasma membrane (2014) · cites this paper as [37]
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- Authors
- Bender, M., Imam, F. B., Talbot, W. S., Ganetzky, B., & Hogness, D. S.
- Affiliations
- University of Georgia (US)
- Stanford Medicine (US)
- University of Wisconsin–Madison (US)
- Corresponding author
- Bender, Michael
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1997-12
- Volume
- 91
- Issue
- 6
- Pages
- 777-788
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2022416579
- Cited by
- 280
- References
- 35
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 4 · 2023: 6 · 2022: 4 · 2021: 4 · 2020: 7 · 2019: 4 · 2018: 2 · 2017: 2 · 2016: 5 · 2015: 8 · 2014: 7 · 2013: 13 · 2012: 10
- FWCI
- 7.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01gm043100-07)
- National Institute of General Medical Sciences (GM-53681, GM43100, R01GM053681)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurobiology and Insect Physiology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Nuclear Receptors and Signaling
- Keywords
- ecdysone receptor, metamorphosis, embryonic lethality, DNA-binding domain, ligand-binding domain, larval salivary gland, Drosophila
- MeSH terms
- Animals, Chromosome Mapping, Drosophila melanogaster, Ecdysone, Exons, Female, Genes, Lethal, Genetic Complementation Test, Male, Metamorphosis, Biological, Receptors, Steroid, Mutagenesis, Genes, Insect
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