Cited by 212RNA Research and SplicingNuclear Structure and FunctionRNA modifications and cancer

A Conserved WD40 Protein Binds the Cajal Body Localization Signal of scaRNP Particles

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Tycowski, Kazimierz T., Shu, Mei-Di, Kukoyi, Abiodun, Steitz, Joan A.·2009·Molecular Cell

Abstract

Small Cajal body (CB)-specific RNPs (scaRNPs) function in posttranscriptional modification of small nuclear (sn)RNAs. An RNA element, the CAB box, facilitates CB localization of H/ACA scaRNPs. Using a related element in Drosophila C/D scaRNAs, we purified a fly WD40 repeat protein that UV crosslinks to RNA in a C/D CAB box-dependent manner and associates with C/D and mixed domain C/D-H/ACA scaRNAs. Its human homolog, WDR79, associates with C/D, H/ACA, and mixed domain scaRNAs, as well as with telomerase RNA. WDR79's binding to human H/ACA and mixed domain scaRNAs is CAB box dependent, and its association with mixed domain RNAs also requires the ACA motif, arguing for additional interactions of WDR79 with H/ACA core proteins. We demonstrate a requirement for WDR79 binding in the CB localization of a scaRNA. This and other recent reports establish WDR79 as a central player in the localization and processing of nuclear RNPs.

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Crossref · 10.1016/j.molcel.2009.02.020

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  • TCAB1 (WDR79) is a WD40-repeat protein that binds dyskerin and the CAB box in scaRNAs.

    “TCAB1 (also known as WDR79) is a WD40-repeat containing protein that associates with dyskerin and binds the CAB box in scaRNAs (46,47).”

    From Telomeres and telomerase in cancer (2010) · cites this paper as [47]

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Authors
Tycowski, K. T., Shu, M.-D., Kukoyi, A., & Steitz, J. A.
Affiliations
Howard Hughes Medical Institute (US)
Corresponding author
Steitz, Joan A.
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2009-04
Volume
34
Issue
1
Pages
47-57
ISSN
1097-2765 (ISSN-L)
OpenAlex
W1998314264
Cited by
212
References
53
Citations by year
2026: 3 · 2025: 12 · 2024: 2 · 2023: 11 · 2022: 7 · 2021: 9 · 2020: 12 · 2019: 10 · 2018: 11 · 2017: 12 · 2016: 16 · 2015: 16 · 2014: 20 · 2013: 13 · 2012: 19
FWCI
7.78
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Howard Hughes Medical Institute
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM026154)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
Keywords
WD40 repeat proteins, Cajal bodies, H/ACA ribonucleoprotein, RNA-protein interactions, telomerase RNA, Drosophila, human
MeSH terms
Animals, Base Sequence, Cell Line, Chromatography, Affinity, Drosophila melanogaster, HeLa Cells, Humans, Molecular Sequence Data, Nucleic Acid Conformation, Recombinant Fusion Proteins, Ribonucleoproteins, Sequence Alignment, RNA-Binding Proteins, Coiled Bodies, Amino Acid Motifs, Drosophila Proteins, Regulatory Sequences, Ribonucleic Acid

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