A Conserved WD40 Protein Binds the Cajal Body Localization Signal of scaRNP Particles
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Small Cajal body (CB)-specific RNPs (scaRNPs) function in posttranscriptional modification of small nuclear (sn)RNAs. An RNA element, the CAB box, facilitates CB localization of H/ACA scaRNPs. Using a related element in Drosophila C/D scaRNAs, we purified a fly WD40 repeat protein that UV crosslinks to RNA in a C/D CAB box-dependent manner and associates with C/D and mixed domain C/D-H/ACA scaRNAs. Its human homolog, WDR79, associates with C/D, H/ACA, and mixed domain scaRNAs, as well as with telomerase RNA. WDR79's binding to human H/ACA and mixed domain scaRNAs is CAB box dependent, and its association with mixed domain RNAs also requires the ACA motif, arguing for additional interactions of WDR79 with H/ACA core proteins. We demonstrate a requirement for WDR79 binding in the CB localization of a scaRNA. This and other recent reports establish WDR79 as a central player in the localization and processing of nuclear RNPs.
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TCAB1 (WDR79) is a WD40-repeat protein that binds dyskerin and the CAB box in scaRNAs.
“TCAB1 (also known as WDR79) is a WD40-repeat containing protein that associates with dyskerin and binds the CAB box in scaRNAs (46,47).”
From Telomeres and telomerase in cancer (2010) · cites this paper as [47]
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- Authors
- Tycowski, K. T., Shu, M.-D., Kukoyi, A., & Steitz, J. A.
- Affiliations
- Howard Hughes Medical Institute (US)
- Corresponding author
- Steitz, Joan A.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2009-04
- Volume
- 34
- Issue
- 1
- Pages
- 47-57
- ISSN
- 1097-2765 (ISSN-L)
- PMC
- PMC2700737
- OpenAlex
- W1998314264
- Semantic Scholar
- 48e0435a48f75aef5d30aa64a5c0120923979bd9
- Cited by
- 212
- References
- 53
- Citations by year
- 2026: 3 · 2025: 12 · 2024: 2 · 2023: 11 · 2022: 7 · 2021: 9 · 2020: 12 · 2019: 10 · 2018: 11 · 2017: 12 · 2016: 16 · 2015: 16 · 2014: 20 · 2013: 13 · 2012: 19
- FWCI
- 7.78
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Howard Hughes Medical Institute
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM026154)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA modifications and cancer
- Keywords
- WD40 repeat proteins, Cajal bodies, H/ACA ribonucleoprotein, RNA-protein interactions, telomerase RNA, Drosophila, human
- MeSH terms
- Animals, Base Sequence, Cell Line, Chromatography, Affinity, Drosophila melanogaster, HeLa Cells, Humans, Molecular Sequence Data, Nucleic Acid Conformation, Recombinant Fusion Proteins, Ribonucleoproteins, Sequence Alignment, RNA-Binding Proteins, Coiled Bodies, Amino Acid Motifs, Drosophila Proteins, Regulatory Sequences, Ribonucleic Acid
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