The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Uptake of Extracellular Double-Stranded RNA by SID-2
Open accessAbstract
Summary Ingested dsRNAs trigger RNA interference (RNAi) in many invertebrates including the nematode Caenorhabditis elegans. Here we show that the C. elegans apical intestinal membrane protein SID-2 is required in C. elegans for the import of ingested dsRNA and, when expressed in Drosophila S2 cells, SID-2 enables the uptake of dsRNAs. SID-2-dependent dsRNA transport requires an acidic extracellular environment and is selective for dsRNAs with at least 50 base pairs. Through structure-function analysis, we identify several SID-2 regions required for this activity including three extracellular, positively-charged, histidines. Finally, we find that SID-2-dependent transport is inhibited by drugs that interfere with vesicle transport. Therefore, we propose that environmental dsRNAs are imported from the acidic intestinal lumen by SID-2 via endocytosis and are released from internalized vesicles in a secondary step mediated by the dsRNA-channel SID-1. Similar multistep mechanisms may underlie the widespread observations of environmental RNAi.
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Cites
RNAi-mediated gene silencing in non-human primates
Zimmermann, T. S., Lee, A. C. H., Akinc, A., Bramlage, B., Bumcrot, D., Fedoruk, M. N., Harborth, J., Heyes, J. A., Jeffs, L. B., John, M., et al. (2006) · Nature · doi:10.1038/nature04688 · Cited by 1,357 · cited 2×
“…ligands such as cholesterol and cell-type specific antibodies mediate an initial SID-2-like step that is capable of transporting siRNAs, this model additionally clarifies why fusing therapeutic siRNAs to these ligands increases their cellular uptake (Wolfrum et al., 2007; Zimmermann et al., 2006).”
Cited by
Bidirectional cross-kingdom RNAi and fungal uptake of external RNAs confer plant protection
Wang, M., Weiberg, A., Lin, F.-M., Thomma, B. P. H. J., Huang, H.-D., & Jin, H. (2016) · Nature Plants · doi:10.1038/nplants.2016.151 · Cited by 801
Mechanisms, Applications, and Challenges of Insect RNA Interference
Zhu, K. Y., & Palli, S. R. (2020) · Annual Review of Entomology · doi:10.1146/annurev-ento-011019-025224 · Cited by 709
Epigenetic inheritance of acquired traits through sperm RNAs and sperm RNA modifications
Chen, Q., Yan, W., & Duan, E. (2016) · Nature Reviews Genetics · doi:10.1038/nrg.2016.106 · Cited by 576
Joga, M. R., Zotti, M. J., Smagghe, G., & Christiaens, O. (2016) · Frontiers in Physiology · doi:10.3389/fphys.2016.00553 · Cited by 521
Veremeyko, T., Kuznetsova, I. S., Dukhinova, M., W. Y. Yung, A., Kopeikina, E., Barteneva, N. S., & Ponomarev, E. D. (2019) · Journal of Neuroscience Research · doi:10.1002/jnr.24344 · Cited by 52
Metadata
- Authors
- McEwan, D. L., Weisman, A. S., & Hunter, C. P.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2012-09
- Volume
- 47
- Issue
- 5
- Pages
- 746-754
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W1987956537
- Cited by
- 193
- References
- 1
- FWCI
- 4.88
- Type
- journal article
- Language
- en
- Open access
- bronze
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
