miR-14 Regulates Autophagy during Developmental Cell Death by Targeting ip3-kinase 2
Open accessAbstract
Macroautophagy (autophagy) is a lysosome-dependent degradation process that has been implicated in age-associated diseases. Autophagy is involved in both cell survival and cell death, but little is known about the mechanisms that distinguish its use during these distinct cell fates. Here, we identify the microRNA miR-14 as being both necessary and sufficient for autophagy during developmentally regulated cell death in Drosophila. Loss of miR-14 prevented induction of autophagy during salivary gland cell death, but had no effect on starvation-induced autophagy in the fat body. Moreover, misexpression of miR-14 was sufficient to prematurely induce autophagy in salivary glands, but not in the fat body. Importantly, miR-14 regulates this context-specific autophagy through its target, inositol 1,4,5-trisphosphate kinase 2 (ip3k2), thereby affecting inositol 1,4,5-trisphosphate (IP3) signaling and calcium levels during salivary gland cell death. This study provides in vivo evidence of microRNA regulation of autophagy through modulation of IP3 signaling.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Developmental degradation of Drosophila salivary glands by autophagy requires Utx, miR-14, Rala, Drpr, and Mcr.
“Moreover, the developmental degradation of Drosophila salivary glands by autophagy requires the activity of: (1) Utx histone demethylase (Utx), which contributes to the transcriptional regulation of apoptosis and autophagy genes [839]; (2) miR-14, which specifically activates autophagy-dependent cell death by modulating multiple IP3-driven signaling pathways upon targeting inositol 1,4,5-triphosph”
From Molecular mechanisms of cell death: recommendations of the nomenclature committee on cell death 2018. (2018) · cites this paper as [840]
Cites
Autophagy: Renovation of Cells and Tissues
Mizushima, N., & Komatsu, M. (2011) · Cell · doi:10.1016/j.cell.2011.10.026 · Cited by 6,467 · see citing works
The versatility and universality of calcium signalling
Berridge, M. J., Lipp, P., & Bootman, M. D. (2000) · Nature Reviews Molecular Cell Biology · doi:10.1038/35036035 · Cited by 5,701 · see citing works
Brennecke, J., Hipfner, D. R., Stark, A., Russell, R. B., & Cohen, S. M. (2003) · Cell · doi:10.1016/s0092-8674(03)00231-9 · Cited by 1,721 · see citing works
Optimization of a GCaMP Calcium Indicator for Neural Activity Imaging
Akerboom, J., Chen, T., Wardill, T. J., Tian, L., Marvin, J. S., Mutlu, S., Calderón, N. C., Esposti, F., Borghuis, B. G., Sun, X. R., et al. (2012) · Journal of Neuroscience · doi:10.1523/jneurosci.2601-12.2012 · Cited by 1,226 · see citing works
Control of Macroautophagy by Calcium, Calmodulin-Dependent Kinase Kinase-β, and Bcl-2
Høyer-Hansen, M., Bastholm, L., Szyniarowski, P., Campanella, M., Szabadkai, G., Farkas, T., Bianchi, K., Fehrenbacher, N., Elling, F., Rizzuto, R., et al. (2007) · Molecular Cell · doi:10.1016/j.molcel.2006.12.009 · Cited by 1,072 · see citing works
The Drosophila MicroRNA Mir-14 Suppresses Cell Death and Is Required for Normal Fat Metabolism
Xu, P., Vernooy, S. Y., Guo, M., & Hay, B. A. (2003) · Current Biology · doi:10.1016/s0960-9822(03)00250-1 · Cited by 1,006 · see citing works
Construction of Transgenic Drosophila by Using the Site-Specific Integrase From Phage C31
Groth, A. C. (2004) · Genetics · doi:10.1534/genetics.166.4.1775 · Cited by 963 · see citing works
Drosophila's Insulin/PI3-Kinase Pathway Coordinates Cellular Metabolism with Nutritional Conditions
Britton, J. S., Lockwood, W. K., Li, L., Cohen, S. M., & Edgar, B. A. (2002) · Developmental Cell · doi:10.1016/s1534-5807(02)00117-x · Cited by 683 · see citing works
Growth Arrest and Autophagy Are Required for Salivary Gland Cell Degradation in Drosophila
Berry, D. L., & Baehrecke, E. H. (2007) · Cell · doi:10.1016/j.cell.2007.10.048 · Cited by 602 · see citing works
Self-citationRegulation of autophagy by a beclin 1-targeted microRNA, miR-30a, in cancer cells
Hua Zhu, C., Wu, H., Liu, X., Li, B., Chen, Y., Ren, X., Liu, C., & Yang, J. (2009) · Autophagy · doi:10.4161/auto.9064 · Cited by 457 · see citing works
Steroid regulated programmed cell death during Drosophila metamorphosis
Jiang, C., Baehrecke, E. H., & Thummel, C. S. (1997) · Development · doi:10.1242/dev.124.22.4673 · Cited by 392 · see citing works
Self-citationmicroRNA‐101 is a potent inhibitor of autophagy
Frankel, L. B., Wen, J., Lees, M. J., Høyer-Hansen, M. H., Farkas, T., Krogh, A., Jäättelä, M., & Lund, A. H. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.331 · Cited by 343 · see citing works
Steroid regulation of autophagic programmed cell death during development
Lee, C.-Y., & Baehrecke, E. H. (2001) · Development · doi:10.1242/dev.128.8.1443 · Cited by 336 · see citing works
Self-citationMicroRNAs and developmental timing
Ambros, V. (2011) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2011.04.003 · Cited by 314 · see citing works
Self-citationAntagonism of Beclin 1‐dependent autophagy by BCL‐2 at the endoplasmic reticulum requires NAF‐1
Chang, N. C., Nguyen, M., Germain, M., & Shore, G. C. (2009) · The EMBO Journal · doi:10.1038/emboj.2009.369 · Cited by 281 · see citing works
Caygill, E. E., & Johnston, L. A. (2008) · Current Biology · doi:10.1016/j.cub.2008.06.020 · Cited by 273 · see citing works
Caspases function in autophagic programmed cell death inDrosophila
Martin, D. N., & Baehrecke, E. H. (2003) · Development · doi:10.1242/dev.00933 · Cited by 258 · see citing works
Self-citationA dual role for Ca2+ in autophagy regulation
Decuypere, J.-P., Bultynck, G., & Parys, J. B. (2011) · Cell Calcium · doi:10.1016/j.ceca.2011.04.001 · Cited by 257 · see citing works
Selective autophagy degrades DICER and AGO2 and regulates miRNA activity
Gibbings, D., Mostowy, S., Jay, F., Schwab, Y., Cossart, P., & Voinnet, O. (2012) · Nature Cell Biology · doi:10.1038/ncb2611 · Cited by 249 · see citing works
Drosophila melanogaster. Practical uses in cell and molecular biology
Datta, S. (1995) · Trends in Genetics · doi:10.1016/s0168-9525(00)89131-3 · Cited by 232 · see citing works
Varghese, J., & Cohen, S. M. (2007) · Genes & Development · doi:10.1101/gad.439807 · Cited by 196 · see citing works
Uba1 functions in Atg7- and Atg3-independent autophagy
Chang, T.-K., Shravage, B. V., Hayes, S. D., Powers, C. M., Simin, R. T., Wade Harper, J., & Baehrecke, E. H. (2013) · Nature Cell Biology · doi:10.1038/ncb2804 · Cited by 187 · see citing works
Self-citationChapter 29 Methods for Quantitative Analysis of Transcription in Larvae and Prepupae
Andres, A. J., & Thummel, C. S. (1994) · Methods in cell biology · doi:10.1016/s0091-679x(08)60932-2 · Cited by 155 · see citing works
Muro, I., Berry, D. L., Huh, J. R., Chen, C., Huang, H., Yoo, S. J., Guo, M., Baehrecke, E. H., & Hay, B. A. (2006) · Development · doi:10.1242/dev.02495 · Cited by 150 · see citing works
Self-citationActivation of autophagy during cell death requires the engulfment receptor Draper
Kary, C. S., Logan, M. A., Freeman, M. R., & Baehrecke, E. H. (2010) · Nature · doi:10.1038/nature09127 · Cited by 138 · see citing works
Self-citationDrosophila miR-14 regulates insulin production and metabolism through its target, sugarbabePreprint
Varghese, J., Lim, S. F., & Cohen, S. M. (2010) · Genes & Development · doi:10.1101/gad.1995910 · Cited by 136 · see citing works
Genomic mapping of binding regions for the Ecdysone receptor protein complexPreprint
Gauhar, Z., Sun, L. V., Hua, S., et al. (2009) · Genome Research · doi:10.1101/gr.081349.108 · Cited by 110 · see citing works
A Molecular Basis for Inositol Polyphosphate Synthesis in Drosophila melanogaster
Seeds, A. M., Sandquist, J. C., Spana, E. P., & York, J. D. (2004) · Journal of Biological Chemistry · doi:10.1074/jbc.m408295200 · Cited by 89 · see citing works
Dutta, S., & Baehrecke, E. H. (2008) · Current Biology · doi:10.1016/j.cub.2008.08.052 · Cited by 61 · see citing works
Self-citationAutophagy modulates miRNA‐mediated gene silencing and selectively degrades AIN‐1/GW182 in C. elegans
Zhang, P., & Zhang, H. (2013) · EMBO Reports · doi:10.1038/embor.2013.53 · Cited by 57 · see citing works
Small temporal RNAs in animal development
Sokol, N. S. (2012) · Current Opinion in Genetics & Development · doi:10.1016/j.gde.2012.04.001 · Cited by 53 · see citing works
Terhzaz, S., Finlayson, A., Stirrat, L., Yang, J., Tricoire, H., Woods, D. J., Dow, J. A. T., & Davies, S. A. (2010) · Cellular Signalling · doi:10.1016/j.cellsig.2009.12.009 · Cited by 45 · see citing works
TARGETED EXPRESSION OF IP3SPONGE AND IP3 DS RNA IMPAIRES SUGAR TASTE SENSATION INDROSOPHILA
Usui‐Aoki, K., Matsumoto, K., Koganezawa, M., et al. (2005) · Journal of Neurogenetics · doi:10.1080/01677060600569713 · Cited by 31 · see citing works
Regulation of Inositol 1,4,5-Trisphosphate 3-Kinases by Calcium and Localization in Cells
Lloyd-Burton, S. M., Yu, J. C. H., Irvine, R. F., & Schell, M. J. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m610253200 · Cited by 21 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Galluzzi, L., Vitale, I., Aaronson, S. A., Abrams, J. M., Adam, D., Agostinis, P., Alnemri, E. S., Altucci, L., Amelio, I., Andrews, D. W., et al. (2018) · Cell Death & Differentiation · doi:10.1038/s41418-017-0012-4 · Cited by 6,680 · see citing works
Self-citationKlionsky, D. J., Abdel-Aziz, A. K., Abdelfatah, S., Abdellatif, M., Abdoli, A., Abel, S., Abeliovich, H., Abildgaard, M. H., Abudu, Y. P., Acevedo-Arozena, A., et al. (2021) · Autophagy · doi:10.1080/15548627.2020.1797280 · Cited by 2,622 · see citing works
Self-citationAutophagy-dependent cell death
Denton, D., & Kumar, S. (2019) · Cell Death & Differentiation · doi:10.1038/s41418-018-0252-y · Cited by 777 · see citing works
Entosis Is Induced by Glucose Starvation
Hamann, J. C., Surcel, A., Chen, R., Teragawa, C., Albeck, J. G., Robinson, D. N., & Overholtzer, M. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.06.037 · Cited by 203 · see citing works
Autophagy in animal development
Allen, E. A., & Baehrecke, E. H. (2020) · Cell Death & Differentiation · doi:10.1038/s41418-020-0497-0 · Cited by 127 · see citing works
Self-citationMechanisms and consequences of entosis
Krishna, S., & Overholtzer, M. (2016) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-016-2207-0 · Cited by 118 · see citing works
Eaten alive: novel insights into autophagy from multicellular model systems
Zhang, H., & Baehrecke, E. H. (2015) · Trends in Cell Biology · doi:10.1016/j.tcb.2015.03.001 · Cited by 102 · see citing works
Self-citationRal GTPase and the exocyst regulate autophagy in a tissue‐specific manner
Tracy, K., Velentzas, P. D., & Baehrecke, E. H. (2016) · The EMBO Reports · doi:10.15252/embr.201541283 · Cited by 22 · see citing works
Self-citation
- Authors
- Nelson, C., Ambros, V., & Baehrecke, E. H.
- Affiliations
- University of Massachusetts Chan Medical School (US)
- Corresponding author
- Baehrecke, Eric H.
- Venue
- Molecular Cell
- Publisher
- Elsevier BV
- Published
- 2014-11
- Volume
- 56
- Issue
- 3
- Pages
- 376-388
- ISSN
- 1097-2765 (ISSN-L)
- OpenAlex
- W1969567490
- Cited by
- 61
- References
- 34
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 4 · 2021: 3 · 2020: 6 · 2019: 6 · 2018: 4 · 2017: 8 · 2016: 4 · 2015: 11 · 2014: 1
- FWCI
- 6.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Molecular Cell (publishedVersion)
- PubMed (publishedVersion)
- The Journal of the American Medical Association (JAMA) Network (American Medical Association) (submittedVersion)
- Funders
- National Institutes of Health (GM079431-04S1, R01GM34028)
- Topics
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- autophagy, developmental cell death, IP3 signaling, calcium signaling, fat body, Drosophila
- MeSH terms
- Animals, Autophagy, Calcium, Cell Line, Drosophila melanogaster, Larva, Salivary Glands, Second Messenger Systems, Inositol 1,4,5-Trisphosphate, Phosphotransferases (Alcohol Group Acceptor), Drosophila Proteins, RNA Interference, MicroRNAs
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
