Cited by 133Genomics and Chromatin DynamicsChromatin Remodeling and CancerNuclear Structure and Function

Sequence-Directed Action of RSC Remodeler and General Regulatory Factors Modulates +1 Nucleosome Position to Facilitate Transcription

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Kubik, Slawomir, O’Duibhir, Eoghan, de Jonge, Wim J., Mattarocci, Stefano, Albert, Benjamin, Falcone, Jean-Luc, Bruzzone, Maria Jessica, Holstege, Frank C.P. +1 more·2018·Molecular Cell

Abstract

Accessible chromatin is important for RNA polymerase II recruitment and transcription initiation at eukaryotic promoters. We investigated the mechanistic links between promoter DNA sequence, nucleosome positioning, and transcription. Our results indicate that positioning of the transcription start site-associated +1 nucleosome in yeast is critical for efficient TBP binding and is driven by two key factors, the essential chromatin remodeler RSC and a small set of ubiquitous general regulatory factors (GRFs). Our findings indicate that the strength and directionality of RSC action on promoter nucleosomes depends on the arrangement and proximity of two specific DNA motifs. This, together with the effect on nucleosome position observed in double depletion experiments, suggests that, despite their widespread co-localization, RSC and GRFs predominantly act through independent signals to generate accessible chromatin. Our results provide mechanistic insight into how the promoter DNA sequence instructs trans-acting factors to control nucleosome architecture and stimulate transcription initiation.

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Crossref · 10.1016/j.molcel.2018.05.030

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  • Recruitment of transcription machinery to promoters is restricted by nucleosomes.

    “Recruitment of transcription machinery to promoters is restricted by nucleosomes19.”
  • Histone chaperones and ATP-dependent chromatin-remodelling complexes are important for gene regulation because they regulate nucleosome dynamics at promoters, restricting or increasing promoter accessibility for recruitment of transcription factors and RNA polymerase II (Pol II).

    “Histone chaperones and ATP-dependent chromatin-remodelling complexes are accordingly important for gene regulation given they regulate nucleosome dynamics at promoters, restricting or increasing promoter accessibility for recruitment of transcription factors and RNA polymerase II (Pol II)19–23.”

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Authors
Kubik, S., O’Duibhir, E., de Jonge, W. J., Mattarocci, S., Albert, B., Falcone, J.-L., Bruzzone, M. J., Holstege, F. C. P., & Shore, D.
Affiliations
  • Institute of Genetics and Genomics in Geneva (CH)
  • University Medical Center Utrecht (NL)
  • University of Geneva (CH)
Corresponding author
Shore, David
Venue
Molecular Cell
Publisher
Elsevier BV
Published
2018-07
Volume
71
Issue
1
Pages
89-102.e5
ISSN
1097-2765 (ISSN-L)
OpenAlex
W2831181062
Cited by
133
References
67
Citations by year
2026: 3 · 2025: 6 · 2024: 19 · 2023: 23 · 2022: 21 · 2021: 22 · 2020: 17 · 2019: 17 · 2018: 5
FWCI
5.42
Type
journal article
Language
en
Open access
Locations
  • Molecular Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Swiss National Science Foundation (31003A_170153)
  • Republic and Canton of Geneva
  • European Research Council (671174)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
nucleosome positioning, transcription initiation, TATA-binding protein, chromatin accessibility, RNA polymerase II recruitment, Saccharomyces cerevisiae, DNA sequence motifs, transcriptional regulation
MeSH terms
Nucleosomes, RNA Polymerase II, Saccharomyces cerevisiae, Transcription, Genetic, Saccharomyces cerevisiae Proteins, Chromatin Assembly and Disassembly

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