Cited by 345Telomeres, Telomerase, and SenescenceGenetics, Aging, and Longevity in Model OrganismsChromosomal and Genetic Variations

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Telomere length measurement-caveats and a critical assessment of the available technologies and tools

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Aubert, Geraldine, Hills, Mark, Lansdorp, Peter M.·2012·Mutation research. Fundamental and molecular mechanisms of mutagenesis

DOI 10.1016/j.mrfmmm.2011.04.003PMC PMC3460641

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Abstract

Studies of telomeres and telomere biology often critically rely on the detection of telomeric DNA and measurements of the length of telomere repeats in either single cells or populations of cells. Several methods are available that provide this type of information and it is often not clear what method is most appropriate to address a specific research question. The major variables that need to be considered are the material that is or can be made available and the accuracy of measurements that is required. The goal of this review is to provide a comprehensive summary of the most commonly used methods and discuss the advantages and disadvantages of each. Methods that start with genomic DNA include telomere restriction fragment (TRF) length analysis, PCR amplification of telomere repeats relative to a single copy gene by Q-PCR or MMQPCR and single telomere length analysis (STELA), a PCR-based approach that accurately measures the full spectrum of telomere lengths from individual chromosomes. A different set of methods relies on fluorescent in situ hybridization (FISH) to detect telomere repeats in individual cells or chromosomes. By including essential calibration steps and appropriate controls these methods can be used to measure telomere repeat length or content in chromosomes and cells. Such methods include quantitative FISH (Q-FISH) and flow FISH which are based on digital microscopy and flow cytometry, respectively. Here the basic principles of various telomere length measurement methods are described and their strengths and weaknesses are highlighted. Some recent developments in telomere length analysis are also discussed. The information in this review should facilitate the selection of the most suitable method to address specific research question about telomeres in either model organisms or human subjects.

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Authors: Aubert, G., Hills, M., & Lansdorp, P. M.
Affiliations: BC Cancer Agency (CA)
Corresponding author: Lansdorp, Peter M.
ORCID: Lansdorp, Peter M.
Venue: Mutation research. Fundamental and molecular mechanisms of mutagenesis
Publisher: Elsevier BV
Published: 2012-02
Volume: 730
Issue: 1-2
Pages: 59-67
ISSN: 0027-5107 (ISSN-L)
1386-1964
DOI: 10.1016/j.mrfmmm.2011.04.003
PMC: PMC3460641
OpenAlex: W2014666122
Cited by: 342
References: 73
Citations by year: 2026: 3 · 2025: 14 · 2024: 22 · 2023: 16 · 2022: 20 · 2021: 25 · 2020: 31 · 2019: 17 · 2018: 32 · 2017: 36 · 2016: 29 · 2015: 29 · 2014: 27 · 2013: 27 · 2012: 11
FWCI: 14.83
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
www.elsevier.com/legal/tdmrep-license (tdm)
doi.org/10.15223/policy-017 (stm-asf)
doi.org/10.15223/policy-037 (stm-asf)
doi.org/10.15223/policy-012 (stm-asf)
doi.org/10.15223/policy-029 (stm-asf)
doi.org/10.15223/policy-004 (stm-asf)
Locations: Mutation research. Fundamental and molecular mechanisms of mutagenesis (publishedVersion)
PubMed (publishedVersion)
Europe PMC (PubMed Central) (acceptedVersion)
PubMed Central (acceptedVersion)
University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
Funders: Canadian Institutes of Health Research
National Institutes of Health (GMH79042, MOP38075)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Telomeres, Telomerase, and Senescence
Life Sciences › Biochemistry, Genetics and Molecular Biology › Aging › Genetics, Aging, and Longevity in Model Organisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Keywords: telomere length measurement, real-time PCR, quantitative fluorescence in situ hybridization, method comparison, telomere biology
MeSH terms: Biotechnology, Flow Cytometry, Genetic Techniques, Humans, Polymorphism, Restriction Fragment Length, Weights and Measures, Polymerase Chain Reaction, Telomere, In Situ Hybridization, Fluorescence

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Telomere length measurement-caveats and a critical assessment of the available technologies and tools

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