Comparative quantification of dietary supplemented neural creatine concentrations with 1H-MRS peak fitting and basis spectrum methods
Abstract
Magnetic resonance spectroscopy (MRS) is an analytical procedure that can be used to non-invasively measure the concentration of a range of neural metabolites. Creatine is an important neurometabolite with dietary supplementation offering therapeutic potential for neurological disorders with dysfunctional energetic processes. Neural creatine concentrations can be probed using proton MRS and quantified using a range of software packages based on different analytical methods. This experiment examines the differences in quantification performance of two commonly used analysis packages following a creatine supplementation strategy with potential therapeutic application. Human participants followed a seven day dietary supplementation regime in a placebo-controlled, cross-over design interspersed with a five week wash-out period. Spectroscopy data were acquired the day immediately following supplementation and analyzed with two commonly-used software packages which employ vastly different quantification methods. Results demonstrate that neural creatine concentration was augmented following creatine supplementation when analyzed using the peak fitting method of quantification (105.9%±10.1). In contrast, no change in neural creatine levels were detected with supplementation when analysis was conducted using the basis spectrum method of quantification (102.6%±8.6). Results suggest that software packages that employ the peak fitting procedure for spectral quantification are possibly mo…
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Two further studies have confirmed that varying supplementation regimes can increase brain creatine.
“two further studies have confirmed varying supplementation regimes can increase brain creatine [18, 19]”
From The effects of creatine supplementation on cognitive performance—a randomised controlled study (2023) · cites this paper as [18]
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- Authors
- Turner, C. E., Russell, B. R., & Gant, N.
- Affiliations
- University of Auckland (NZ)
- Corresponding author
- Gant, Nicholas
- Venue
- Magnetic Resonance Imaging
- Publisher
- Elsevier BV
- Published
- 2015-11
- Volume
- 33
- Issue
- 9
- Pages
- 1163-1167
- ISSN
- 0730-725X (ISSN-L)
- OpenAlex
- W1414769819
- Cited by
- 19
- References
- 26
- Citations by year
- 2026: 5 · 2024: 1 · 2023: 4 · 2022: 1 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2016: 2
- FWCI
- 1.62
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Magnetic Resonance Imaging (publishedVersion)
- PubMed (publishedVersion)
- ResearchSpace (University of Auckland) (submittedVersion)
- Funders
- University of Auckland
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Physical Sciences › Chemistry › Spectroscopy › Advanced NMR Techniques and Applications
- Keywords
- creatine supplementation, 1H NMR, spectral quantification, peak fitting, software comparison, neurometabolites, dietary supplementation, neurological disorders
- MeSH terms
- Adult, Creatine, Double-Blind Method, Female, Humans, Image Processing, Computer-Assisted, Male, Middle Aged, Cross-Over Studies, Dietary Supplements, Young Adult, Proton Magnetic Resonance Spectroscopy
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