Cited by 60Nanoparticle-Based Drug DeliveryRNA Interference and Gene DeliveryCharacterization and Applications of Magnetic Nanoparticles

Study and evaluation of nucleolin-targeted delivery of magnetic PLGA-PEG nanospheres loaded with doxorubicin to C6 glioma cells compared with low nucleolin-expressing L929 cells

Mosafer, Jafar, Teymouri, Manouchehr, Abnous, Khalil, Tafaghodi, Mohsen, Ramezani, Mohammad·2017·Materials Science and Engineering: C

Abstract

Magnetic nanoparticulate systems based on polymeric materials such as poly (lactic-co-glycolic acid) (PLGA 1) are being studied for their potential applications in targeted therapy and imaging of malignant tumors. In the current study, superparamagnetic iron oxide nanocrystals (SPIONs 2) and doxorubicin (Dox 3) were entrapped in the PLGA-based nanoparticles via a modified multiple emulsion solvent evaporation method. Furthermore, SPIO/Dox-NPs 4 were conjugated to anti-nucleolin AS1411 aptamer (Apt 5) and their targeting ability was investigated in high nucleolin-expressing C6 glioma cells compared to low nucleolin-expressing L929 cells. The NPs exhibited a narrow size distribution with mean diameter of ~170nm and an appropriate SPION content (~18% of total polymer weight) with a sufficient saturation magnetization value of 5.9emu/g which is suitable for imaging objectives. They manifested an increased Dox release at pH5.5 compared to pH7.4, with initial burst release (within 24h) followed by sustained release of Dox for 36days. The Apt conjugation to NPs enhanced cellular uptake of Dox in C6 glioma cells compared to L929 cells. Similarly, the Apt-NPs increased the cytotoxicity effect of Dox compared with NPs and Dox solution (f-Dox) alone. In conclusion, the Apt-NPs were found to be a promising delivery system for therapeutic and diagnostic purposes.

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Crossref · 10.1016/j.msec.2016.11.053

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Authors
Mosafer, J., Teymouri, M., Abnous, K., Tafaghodi, M., & Ramezani, M.
Affiliations
  • Mashhad University of Medical Sciences (IR)
  • Gonabad University of Medical Sciences (IR)
Corresponding author
Ramezani, Mohammad
Venue
Materials Science and Engineering: C
Publisher
Elsevier BV
Published
2017-03
Volume
72
Pages
123-133
ISSN
0928-4931 (ISSN-L)
OpenAlex
W2553894721
Cited by
60
References
43
Citations by year
2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 6 · 2021: 7 · 2020: 3 · 2019: 13 · 2018: 12 · 2017: 6
FWCI
4.23
Type
journal article
Language
en
Open access
closed
Locations
  • Materials Science and Engineering C (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
  • Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
Keywords
nucleolin, AS1411 aptamer, targeted drug delivery, PLGA-PEG nanoparticles, superparamagnetic iron oxide nanoparticles, doxorubicin, C6 glioma cells, L929 fibroblasts, pH-responsive drug release, cellular uptake, cytotoxicity
MeSH terms
Polylactic Acid-Polyglycolic Acid Copolymer, Nucleolin, Animals, Antibiotics, Antineoplastic, Cell Survival, Dextrans, Doxorubicin, Drug Carriers, Glioma, Half-Life, Immunohistochemistry, Particle Size, Phosphoproteins, Polyethylene Glycols, Polyglycolic Acid, RNA-Binding Proteins, Lactic Acid, Cell Line, Tumor, Mice, Rats, Aptamers, Nucleotide, Nanospheres, Magnetite Nanoparticles, Drug Liberation

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