Novel bone-mimetic nanohydroxyapatite/collagen porous scaffolds biomimetically mineralized from surface silanized mesoporous nanobioglass/collagen hybrid scaffold: physicochemical, mechanical and in vivo evaluations
Abstract
Bone-mimetic scaffolds are receiving much interest as such scaffolds exhibit excellent biocompatibility and very close mimic to bone structure and composition. Here, novel bone-mimetic nanohydroxyapatite (nHA)/collagen (Col) porous scaffolds (nHA/Col) were prepared from surface silanized mesoporous nanobioglass (NBG)/Col hybrid scaffold by biomimetic mineralization. Surface silanized mesoporous NBG was prepared by ultrasound-assisted sol-gel method and post treatment with 3-aminopropyltriethylsilane (APTS). The surface silanized mesoporous NBG was characterized by transmission electron microscopy (TEM), transmission electron microscopy-selected area electron diffraction (TEM-SAED) and X-ray photoelectron spectroscopy (XPS). The physicochemical/mechanical characterizations of the scaffolds included scanning electron microscopy (SEM) and TEM imaging of micro/nanostructure, energy dispersive X-ray (EDX) analysis of chemical composition, TEM-SAED and X-ray diffraction/Attenuated total Reflectance-Fourier Infrared spectroscopy (XRD/ATR-FTIR) analyses of amorphous-to-crystalline transformations, thermogravimetric/differential scanning calorimetric (TGA/DSC) analyses of thermal behaviour , porosity and dynamic mechanical analyses. The presence of NBG in collagen fibrillar network enabled progressive growth of HA nanocrystals and generation of a novel bone-mimetic hybrid structures while preserving the highly porous structure of collagen scaffold. The crystallinity, crystallite size and crystal morphology of the grown HA nanocrystals were controllable by regulation of the mineralization time. Furthermore, the osteogenic properties of the non-mineralized (NBG/Col) and mineralized (nHA/Col) hybrid porous scaffolds were examined in vivo using critical-sized calvarial bone defect model in rat. Histological and micro-computed tomography (Micro-CT) analyses after 6 weeks of implantation revealed that the mineralized scaffolds possess excellent in vivo osteogenic potential compared to the non-mineralized one. Collectively, by using surface silanized mesoporous NBG hybridization with collagen fibrillar network, we successfully introduced a new approach for developing novel bone-mimetic nanohydroxyapatite/collagen hybrid scaffolds that possess significant potential for bone tissue regeneration.
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Nano-HA/collagen scaffold displayed better bone formation ability.
“Nano-HA/collagen scaffold [97] | displayed better bone formation | ability.”
From Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration. (2023) · cites this paper as [97]
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Nano-hydroxyapatite/collagen composite as scaffold material for bone regeneration.
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- Authors
- El-Fiqi, A., Kim, J.-H., & Kim, H.-W.
- Affiliations
- Dankook University (KR)
- Corresponding author
- Kim, Hae-Won
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2020-05
- Volume
- 110
- Pages
- 110660
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W3000603405
- Cited by
- 66
- References
- 54
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 10 · 2023: 11 · 2022: 10 · 2021: 17 · 2020: 8
- FWCI
- 3.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation of Korea (2015032163, 2018K1A4A3A01064257)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Materials Chemistry › Mesoporous Materials and Catalysis
- Keywords
- hydroxyapatite nanoparticles, collagen, surface silanization, biomimetic mineralization, hybrid scaffolds, porous scaffolds, bone regeneration, osteogenic differentiation, calvarial defect
- MeSH terms
- Animals, Bone and Bones, Calorimetry, Differential Scanning, Ceramics, Collagen, Materials Testing, Osteogenesis, Silanes, Skull, X-Ray Diffraction, Porosity, Spectroscopy, Fourier Transform Infrared, Durapatite, Biomimetic Materials, Rats, Tissue Scaffolds, X-Ray Microtomography, Photoelectron Spectroscopy
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