Biofabrication of poly(l-lactide-co-ε-caprolactone)/silk fibroin scaffold for the application as superb anti-calcification tissue engineered prosthetic valve
Abstract
In this study, electrospun scaffolds were fabricated by blending poly(l-lactide-co-ε-caprolactone) (PLCL) and silk fibroin (SF) with different ratios, and further the feasibility of electrospun PLCL/SF scaffolds were evaluated for application of tissue engineered heart valve (TEHV). Scanning electron microscopy (SEM) results showed that the surface of PLCL/SF electrospun scaffolds was smooth and uniform while the mechanical properties were appropriate as valve prosthesis. In vitro cytocompatibility evaluation results demonstrated that all of the PLCL/SF electrospun scaffolds were cytocompatible and valvular interstitial cells (VICs) cultured on PLCL/SF scaffolds of 80/20 & 70/30 ratios exhibited the best cytocompatibility. The in vitro osteogenic differentiation of VICs including alkaline phosphatase (ALP) activity and quantitative polymerase chain reaction (qPCR) assays indicated that PLCL/SF scaffolds of 80/20 & 90/10 ratios behaved better anti-calcification ability. In the in vivo calcification evaluation model of rat subdermal implantation, PLCL/SF scaffolds of 80/20 & 90/10 ratios presented better anti-calcification ability, which was consistent with the in vitro results. Moreover, PLCL/SF scaffolds of 80/20 & 70/30 ratios showed significantly enhanced cell infiltration and M2 macrophage with higher CD206+/CD68+ ratio. Collectively, our data demonstrated that electrospun scaffolds with the PLCL/SF ratio of 80/20 hold great potential as TEHV materials.
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PLCL/SF scaffold has good biocompatibility and anti-calcification ability.
“in vitro experiments showed that the PLCL/SF scaffold had good biocompatibility and anti-calcification ability [174].”
From Application of PLCL as a biodegradable polymer in biomedical engineering (2026) · cites this paper as [174]
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Wang, X., Jiang, H., Zhang, W., Kong, Y., Kong, D., Liu, J., & Wang, Z. (2023) · Journal of Materials Chemistry B · doi:10.1039/d3tb00761h · Cited by 7 · see citing works
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- Authors
- Wang, X., Liu, J., Jing, H., Li, B., Sun, Z., Li, B., Kong, D., Leng, X., & Wang, Z.
- Affiliations
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Corresponding author
- Liu, Jing
- Leng, Xigang
- Wang, Zhihong
- Venue
- Materials Science and Engineering: C
- Publisher
- Elsevier BV
- Published
- 2021-02
- Volume
- 121
- Pages
- 111872
- ISSN
- 0928-4931 (ISSN-L)
- OpenAlex
- W3118696035
- Cited by
- 25
- References
- 64
- Citations by year
- 2026: 8 · 2025: 4 · 2024: 6 · 2023: 5 · 2022: 2
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Materials Science and Engineering C (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China (31771059, 31800815)
- Chinese Academy of Medical Sciences
- Fundamental Research Funds for the Central Universities (3332018119)
- Chinese Academy of Medical Sciences Initiative for Innovative Medicine (2017-12M-3-002)
- Tianjin Science and Technology Program (19YFZCSY00520, TJSQNTJ-2018-02)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Electrospun Nanofibers in Biomedical Applications
- Physical Sciences › Materials Science › Biomaterials › Silk-based biomaterials and applications
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Keywords
- tissue-engineered heart valves, electrospun scaffolds, poly(L-lactide-co-ε-caprolactone), silk fibroin, anticalcification, valvular interstitial cells, osteogenic differentiation, macrophage polarization, M2 macrophages, cytocompatibility, biodegradable polymer blends, subcutaneous implantation
- MeSH terms
- Animals, Aortic Valve, Aortic Valve Stenosis, Calcinosis, Caproates, Cells, Cultured, Dioxanes, Fibroins, Lactones, Osteogenesis, Polyesters, Tissue Engineering, Rats, Tissue Scaffolds
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