The enhancement of siPLK1 penetration across BBB and its anti glioblastoma activity in vivo by magnet and transferrin co-modified nanoparticle
Abstract
In order to enhance the penetration of small interference RNA against the polo-like kinase I (siPLK1) across BBB to treat glioblastoma (GBM), transferrin (Tf) modified magnetic nanoparticle (Tf-PEG-PLL/MNP@siPLK1) was prepared. The in vitro experiments indicated that Tf-PEG-PLL/MNP@siPLK1 enhanced the cellular uptake of siPLK1, which resulted in an increase of gene silencing effect and cytotoxicity of Tf-PEG-PLL/MNP@siPLK1 on U87 cells. Besides, Tf-PEG-PLL/MNP@siPLK1 significantly inhibited the growth of U87 glioblastoma spheroids and markedly increased the BBB penetration efficiency of siPLK1 with the application of external magnetic field in in-vitro BBB model. The in vivo experiments indicated that siPLK1 selectively accumulated in the brain tissue, and markedly reduced tumor volume and prolonged the survival time of GBM-bearing mice after Tf-PEG-PLL/MNP@siPLK1 was injected to GBM-bearing mice via tail vein. The above data indicated that magnet and transferrin co-modified nanoparticle enhanced siPLK1 penetration across BBB and increased its anti GBM activity in vivo.
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Tf-modified magnetic nanoparticles delivering siPLK1 increase uptake by U87 cells, improve BBB penetration, accumulate in brain tissue, inhibit glioblastoma growth, and prolong survival.
“Liu et al. prepared transferrin (Tf) modified magnetic nanoparticles for the delivery of siRNA (siPLK1). The uptake of siPLK1 by U87 cells can significantly increase, improve the BBB penetration efficiency of siPLK1 in vivo, and can selectively accumulate in the brain tissue, thereby inhibiting the growth of glioblastoma tumors and prolonging the survival period.270”
From Smart nanoparticles for cancer therapy (2023) · cites this paper as [270]
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- Authors
- Liu, D.-Z., Cheng, Y., Cai, R.-Q., Wang, B., Cui, H., Liu, M., Zhang, B.-L., Mei, Q.-B., & Zhou, S.-Y.
- Affiliations
- Air Force Medical University (CN)
- Venue
- Nanomedicine: Nanotechnology, Biology and Medicine
- Publisher
- Elsevier BV
- Published
- 2018-04
- Volume
- 14
- Issue
- 3
- Pages
- 991-1003
- ISSN
- 1549-9634 (ISSN-L)
- OpenAlex
- W2783481949
- Cited by
- 66
- References
- 35
- Citations by year
- 2026: 2 · 2025: 6 · 2024: 9 · 2023: 7 · 2022: 16 · 2021: 11 · 2020: 7 · 2019: 3 · 2018: 5
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Nanomedicine Nanotechnology Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Nature Science Foundation (81641185)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- PLK1, glioblastoma, blood-brain barrier penetration, magnetic nanoparticles, transferrin, siRNA delivery, gene silencing, external magnetic field, U87 cells
- MeSH terms
- Polo-Like Kinase 1, Animals, Blood-Brain Barrier, Cell Cycle, Cell Survival, Endocytosis, Glioblastoma, Particle Size, Proto-Oncogene Proteins, Transferrin, Protein Serine-Threonine Kinases, Cell Cycle Proteins, Gene Silencing, RNA, Small Interfering, Mice, Magnetite Nanoparticles
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