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Cited by 73Barrier Structure and Function StudiesNitric Oxide and Endothelin EffectsCell Adhesion Molecules Research

Hypoxia-induced changes in tight junction permeability of brain capillary endothelial cells are associated with IL-1beta and nitric oxide

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YAMAGATA, K, TAGAMI, M, TAKENAGA, F, YAMORI, Y, ITOH, S·2004·Neurobiology of Disease
DOI 10.1016/j.nbd.2004.08.001
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Abstract

We examined whether hypoxia alone could produce changes in the permeability of brain capillary endothelial cells (EC) and whether a stimulation of hypoxic status alters the gene expression of occludin and glucose transporter 1 (GLUT1). Exposure of EC to hypoxia resulted in increased permeability, with the greatest decrease in transendothelial electrical resistance (TER) at 40 h. Moreover, hypoxia alone induced the expression of both mRNA in EC. Furthermore, we found that interleukin-1 (IL-1)beta, glutamate, hydrogen peroxide (H2O2), and sodium nitroprusside (SNP) induced the expression of mRNA for occludin and GULT1 under normoxic condition. The decrease in TER due to hypoxia was inhibited on addition of an anti-IL1 antibody and nitric oxide synthase (NOS) inhibitor in EC. These results indicate that the expression of occludin and GLUT1 mRNA is sensitive to exposure to hypoxia and that the changes of permeability in EC are associated with IL-1beta and NO.

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Crossref · 10.1016/j.nbd.2004.08.001

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  • ROS and NO can reorganize the cytoskeleton of endothelial cells and modulate tight junction proteins claudin-5 and occludin.

    “In addition, ROS and NO can also reorganize the cytoskeleton of ECs and modulate tight junction proteins claudin-5 and occludin (Yamagata et al., 2004; Schreibelt et al., 2007) activate microglial cells; and up-regulate inflammatory mediators in the first 48 h.”

    From The impact of microglial activation on blood-brain barrier in brain diseases (2014) · cites this paper as Yamagata et al., 2004

  • Microglial-induced late-phase BBB disruption is due to direct effects on endothelial cells: IL-1β and TNF-α downregulate tight junction protein expression in ECs, and together with IL-6 modulate expression of adhesion molecules ICAM-1 and VCAM.

    “Apart from the effects of MMPs, microglial-induced late-phase BBB disruption is due to direct effects on ECs: IL-1β and TNF-α downregulate tight junction proteins expression in ECs (Yamagata et al., 2004; Jiao et al., 2011) and, together with IL-6, modulate the expression of adhesion molecules (ICAM-1 and VCAM; Hallenbeck, 2002).”

    From The impact of microglial activation on blood-brain barrier in brain diseases (2014) · cites this paper as Yamagata et al., 2004

  • ROS and NO activate microglial cells and up-regulate inflammatory mediators in the first 48 h.

    “In addition, ROS and NO can also reorganize the cytoskeleton of ECs and modulate tight junction proteins claudin-5 and occludin (Yamagata et al., 2004; Schreibelt et al., 2007) activate microglial cells; and up-regulate inflammatory mediators in the first 48 h.”

    From The impact of microglial activation on blood-brain barrier in brain diseases (2014) · cites this paper as Yamagata et al., 2004

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Authors
YAMAGATA, K., TAGAMI, M., TAKENAGA, F., YAMORI, Y., & ITOH, S.
Affiliations
  • Nihon University (JP)
  • Sanraku Hospital (JP)
  • World Health Organization (CH)
Corresponding author
YAMAGATA, K
ORCID
Venue
Neurobiology of Disease
Publisher
Elsevier BV
Published
2004-12
Volume
17
Issue
3
Pages
491-499
ISSN
0969-9961 (ISSN-L)
DOI
10.1016/j.nbd.2004.08.001
OpenAlex
W2018575684
Cited by
73
References
62
Citations by year
2025: 2 · 2024: 2 · 2023: 3 · 2022: 3 · 2021: 7 · 2020: 1 · 2019: 4 · 2018: 6 · 2017: 5 · 2016: 4 · 2015: 4 · 2014: 3 · 2013: 4 · 2012: 3
FWCI
1.41
Type
journal article
Language
en
Open access
Licence
Locations
  • Neurobiology of Disease (publishedVersion)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
Keywords
hypoxia, brain microvascular endothelial cells, tight junction permeability, occludin, glucose transporter 1, IL-1β, nitric oxide, nitric oxide synthase, transendothelial electrical resistance, hydrogen peroxide, glutamate, blood-brain barrier
MeSH terms
Animals, Brain, Capillaries, Cell Membrane Permeability, Cells, Cultured, Cerebrovascular Circulation, Electrophysiology, Endothelium, Vascular, Extracellular Space, Interleukin-1, Nitric Oxide, Swine, Cell Hypoxia, Polymerase Chain Reaction, Tight Junctions, NG-Nitroarginine Methyl Ester

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