Increases in cholinergic neurotransmission measured by using choline-sensitive microelectrodes: Enhanced detection by hydrolysis of acetylcholine on recording sites?
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Previous experiments demonstrated that second-based transient increases in choline concentrations measured by electrodes coated with choline oxidase (ChOx) and the amperometric detection of hydrogen peroxide validly indicate the depolarization-dependent release of acetylcholine (ACh) and its hydrolysis by endogenous acetylcholinesterase (AChE). Therefore, choline-sensitive microelectrodes have become valuable tools in neuropharmacological and behavioral research. The present experiments were designed to test the possibility that co-immobilization of ChOx plus AChE on recording sites increases the level of detection for evoked ACh release in the brain. If newly released ACh is not completely hydrolyzed by endogenous AChE and capable of reaching the extracellular space, currents recorded via sites equipped with both enzymes should be greater when compared with sites coated with ChOx only. Pairs of platinum-recording sites were coated either with AChE plus ChOx or ChOx alone. Potassium or nicotine-evoked currents were recorded throughout the entire dorsal-ventral extent of the medial prefrontal cortex (mPFC). The amplitudes of evoked cholinergic signals did not differ significantly between AChE+ChOx and ChOx-only coated recording sites. Additional experiments controlling for several potential confounds suggested that, in vivo, ACh levels > or =150fmol were detected by recordings sites featuring dual enzyme coating. Collectively, these results indicate that co-coating of microelectrodes with AChE does not enhance the detection of cholinergic activity in the cortex compared with measurements via recording sites coated only with ChOx.
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Amperometric measurement using enzyme-coated microelectrodes allows monitoring ACh release at a sub-second resolution.
“The use of enzyme-coated microelectrodes for the amperometric measurement allows monitoring ACh release at a sub-second resolution and thus permits the demonstration of changes in ACh release in association with specific task events or behavioral responses (for evidence indicating the validity of this method see Burmeister et al, 2000; Parikh et al, 2004; Giuliano et al, 2008).”
From Modes and models of forebrain cholinergic neuromodulation of cognition (2011) · cites this paper as Giuliano et al, 2008
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Modes and models of forebrain cholinergic neuromodulation of cognition
Hasselmo, M. E., & Sarter, M. (2011) · Neuropsychopharmacology · doi:10.1038/npp.2010.104 · Cited by 733 · see citing works
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- Authors
- Giuliano, C., Parikh, V., Ward, J. R., Chiamulera, C., & Sarter, M.
- Affiliations
- University of Verona (IT)
- University of Michigan (US)
- Corresponding author
- Sarter, Martin
- Venue
- Neurochemistry International
- Publisher
- Elsevier BV
- Published
- 2008-06
- Volume
- 52
- Issue
- 7
- Pages
- 1343-1350
- ISSN
- 0197-0186 (ISSN-L)
- PMC
- PMC2413416
- OpenAlex
- W2090265061
- Cited by
- 41
- References
- 39
- Citations by year
- 2025: 1 · 2023: 1 · 2022: 2 · 2021: 2 · 2020: 3 · 2019: 4 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 4 · 2013: 4 · 2012: 2
- FWCI
- 2.39
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Neurochemistry International (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health (K02 NH 01072)
- National Institute of Mental Health (K02MH001072, R03MH073600)
- Topics
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Physical Sciences › Chemistry › Electrochemistry › Electrochemical Analysis and Applications
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neural Engineering
- Keywords
- choline oxidase, acetylcholinesterase, acetylcholine release, acetylcholine hydrolysis, medial prefrontal cortex, potassium-evoked release, amperometric detection, hydrogen peroxide
- MeSH terms
- Acetylcholine, Alcohol Oxidoreductases, Animals, Calibration, Choline, Enzymes, Immobilized, Hydrolysis, Immunohistochemistry, Male, Microelectrodes, Synaptic Transmission, Nicotine, Parasympathetic Nervous System, Potassium Chloride, Rats, Inbred F344, Signal Transduction, Tissue Fixation, Nicotinic Agonists, Rats
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