CoChat
Sign Up
Cited by 239Neuroscience and Neuropharmacology ResearchCholinesterase and Neurodegenerative DiseasesNicotinic Acetylcholine Receptors Study

An atlas of the regional and laminar distribution of choline acetyltransferase immunoreactivity in rat cerebral cortex

Lysakowski, A., Wainer, B.H., Bruce, G., Hersh, L.B.·1989·Neuroscience
DOI 10.1016/0306-4522(89)90180-2
Open this paper in CoChat — free account

Abstract

The distribution of cholinergic fibers in rat cortex was investigated using choline acetyl-transferase immunohistochemistry. Previous studies have either shown differences in distribution, but have been limited to selected areas, or have shown no discernable differences between different cortical areas. In our study, we examined all areas of rat cortex and found that there are striking interareal and interlaminar differences in cholinergic fiber distribution. We have found that certain functionally similar cortical areas (e.g. sensory, motor, etc.) have similar patterns of cholinergic innervation and we have designated 13 general patterns of cortical cholinergic innervation. We have also compared, on an area-by-area basis, the pattern of acetylcholinesterase reactivity to that of choline acetyltransferase immunoreactivity, since acetylcholinesterase has been used for many years as a putative cholinergic marker. We found that in most cortical areas, the distribution of acetylcholinesterase-positive fibers paralleled that of choline acetyltransferase-immunoreactive fibers; however, there were some striking differences, notably primary somatosensory (the "barrelfield"), retrosplenial and cingulate cortices. In some areas, a revised concept of rat cortical organization, using cytoarchitectonics, was required. The results of this study provide a comprehensive microscopic analysis of cholinergic fiber innervation of the rat cortex. These results are discussed in relation to previous anatomical, physiological and pharmacological studies of cortical cholinergic innervation. The possible sources of this innervation are also discussed.

Integrity signals

Registry

Crossref · 10.1016/0306-4522(89)90180-2

Cites

148 of 148 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The entire cortex and hippocampus are innervated by cholinergic projections that originate from several regions in the basal forebrain.

    “The entire cortex and hippocampus are innervated by cholinergic projections that originate from several regions in the basal forebrain (Rye et al, 1984; Lysakowski et al, 1989; Mesulam et al, 1992; Kitt et al, 1994).”

    From Modes and models of forebrain cholinergic neuromodulation of cognition (2011) · cites this paper as Lysakowski et al, 1989

Cites

Flagged first, then by citations

2 of the 150 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Related

Authors
Lysakowski, A., Wainer, B. H., Bruce, G., & Hersh, L. B.
Affiliations
  • University of Chicago (US)
  • University of Texas Health Science Center at Dallas (US)
Corresponding author
Lysakowski, A.
ORCID
Venue
Neuroscience
Publisher
Elsevier BV
Published
1989-01
Volume
28
Issue
2
Pages
291-336
ISSN
0306-4522 (ISSN-L)
DOI
10.1016/0306-4522(89)90180-2
OpenAlex
W1969045806
Cited by
239
References
150
Citations by year
2026: 3 · 2024: 2 · 2023: 1 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 4 · 2018: 7 · 2017: 2 · 2016: 5 · 2015: 1 · 2014: 8 · 2013: 7 · 2012: 5
FWCI
4.94
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5t32ns007195-10)
  • National Institute of Neurological Disorders and Stroke (5T32 NS-07195, NS 17661)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (HD 04583)
Topics
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Health Sciences › Medicine › Pharmacology › Cholinesterase and Neurodegenerative Diseases
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
Keywords
choline acetyltransferase, acetylcholinesterase, cholinergic innervation, cerebral cortex, laminar distribution, cortical cytoarchitecture, barrel cortex, retrosplenial cortex, cingulate cortex, rats
MeSH terms
Animals, Brain Mapping, Cerebral Cortex, Choline O-Acetyltransferase, Electrophysiology, Histocytochemistry, Immunohistochemistry, Parasympathetic Nervous System, Rats, Inbred Strains, Tissue Distribution, Rats

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review