Evidence that brain nitric oxide inhibition increases metabolic cost of exercise, reducing running performance in rats
Abstract
To assess the role of nitric oxide (NO) in the metabolic rate and running performance of rats submitted to exercise on a treadmill, 1.43 micromol (2 microL) of Nomega-nitro-L-arginine methyl ester (L-NAME, n=6), a NO synthase inhibitor, or 2 microL of 0.15M NaCl (SAL, n=6) was injected into the lateral cerebral ventricle of male Wistar rats immediately before the animals started running (18m min(-1), 5% inclination). Oxygen consumption (VO2) was measured at rest, during the exercise until fatigue and thereafter during the 30 min of recovery using the indirect calorimetry system. Mechanical efficiency (ME) was also calculated during the running period. During the first 11 min of exercise, there was a similar increase in VO2 while ME remained the same in both groups. Thereafter, VO2 remained stable in the SAL group but continued to increase and remained higher in the L-NAME group until fatigue. The L-NAME-treated rats also showed a sharper decrease in ME than controls. In addition, there was a significant reduction in workload performance by L-NAME-treated animals compared to SAL-treated animals. This suggests that central blockage of nitric oxide increases metabolic cost during exercise, reduces mechanical efficiency and decreases running performance in rats.
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In vivo, NOS inhibition increases oxygen consumption in dogs and rats.
“In vivo, NOS inhibition, which would reduce endogenous NO production, increases oxygen consumption () in dogs [20] and rats [21]”
From Dietary Nitrate Supplementation and Exercise Performance (2014) · cites this paper as [21]
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- Authors
- Lacerda, A. C. R., Marubayashi, U., Balthazar, C. H., & Coimbra, C. C.
- Affiliations
- Universidade Federal de Minas Gerais (BR)
- Corresponding author
- Coimbra, Cândido C.
- Venue
- Neuroscience Letters
- Publisher
- Elsevier BV
- Published
- 2006-01
- Volume
- 393
- Issue
- 2-3
- Pages
- 260-263
- ISSN
- 0304-3940 (ISSN-L)
- OpenAlex
- W1988663323
- Cited by
- 44
- References
- 34
- Citations by year
- 2024: 1 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 1 · 2017: 1 · 2016: 1 · 2015: 5 · 2014: 2 · 2013: 1 · 2012: 4
- FWCI
- 8.94
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Neuroscience Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Fundação de Amparo à Pesquisa do Estado de Minas Gerais
- Topics
- Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
- Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
- Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
- Keywords
- nitric oxide, L-NAME, nitric oxide synthase inhibition, exercise metabolism, oxygen consumption, mechanical efficiency, running performance, exercise fatigue, central nervous system, treadmill running, rats
- MeSH terms
- Animals, Behavior, Animal, Brain, Enzyme Inhibitors, Exercise Test, Male, Nitric Oxide, Oxygen Consumption, Physical Conditioning, Animal, Running, Time Factors, Rats, Wistar, NG-Nitroarginine Methyl Ester, Rats
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