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Cited by 24Neuroinflammation and Neurodegeneration MechanismsCellular Mechanics and InteractionsNeuroscience and Neuropharmacology Research

An artificial three-dimensional matrix promotes ramification in the microglial cell-line, BV-2

Pöttler, Marina, Zierler, Susanna, Kerschbaum, Hubert H.·2006·Neuroscience Letters
DOI 10.1016/j.neulet.2006.09.082
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Abstract

Three-dimensional (3D) cell culture yields strikingly different cell phenotypes compared to two-dimensional (2D) cell culture. Since microglia, monocyte derived immune cells in the brain, exist in a variety of cell shapes ranging from amoeboid to ramified, we evaluated the impact of 2D versus 3D culture conditions on cell shape. The microglial cell-line, BV-2, was either cultured on poly-D-lysine coated dishes (2D culture conditions) or in a BD Pura Matrix Peptide Hydrogel (3D culture conditions) in the absence or presence of the extracellular matrix proteins, fibronectin and collagen type I, respectively. We identified five distinct morphological phenotypes (amoeboid, bipolar, tripolar, multipolar, ramified) and compared the frequency distribution of these phenotypes under different culture conditions using a chi(2) test. Culture of BV-2 cells in an inert 3D matrix shifted the frequency distribution from an amoeboid dominated population, which is typical for BV-2 cells cultured under conventional 2D conditions, to a population dominated by multipolar phenotypes. Fibronectin or collagen type I significantly suppressed matrix-induced ramification. These cell culture experiments illustrate the dependency of cell shape on spatial distribution of potential adhesion sites.

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  • 3D Matrix hydrogels support microglia to grow and maintain ramified morphology and enable microglial response tests to proinflammation stimulus.

    “For example, 3D Matrix hydrogels were reported to support microglia to grow and maintain ramified morphology and enable the microglial response tests to proinflammation stimulus.24, 25”

    From Acoustofluidic assembly of 3D neurospheroids to model Alzheimer's disease (2020) · cites this paper as [24]

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  1. Del-Rio Hortega, 1932, Microglia, vol. 2, 481

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Authors
Pöttler, M., Zierler, S., & Kerschbaum, H. H.
Affiliations
University of Salzburg (AT)
Corresponding author
Kerschbaum, Hubert H.
ORCID
Venue
Neuroscience Letters
Publisher
Elsevier BV
Published
2006-12
Volume
410
Issue
2
Pages
137-140
ISSN
0304-3940 (ISSN-L)
DOI
10.1016/j.neulet.2006.09.082
OpenAlex
W1977167767
Cited by
24
References
17
Citations by year
2025: 2 · 2024: 1 · 2023: 2 · 2021: 4 · 2020: 2 · 2018: 2 · 2017: 2 · 2016: 1 · 2014: 3 · 2013: 1 · 2012: 2
FWCI
0.44
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Neuroscience Letters (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
microglia, 3D cell culture, cell morphology, ramified microglia, cell shape, extracellular matrix, fibronectin, type I collagen, poly-D-lysine, cell adhesion
MeSH terms
Animals, Cell Count, Cell Line, Extracellular Matrix, Fibronectins, Chi-Square Distribution, Microglia, Cell Culture Techniques, Collagen Type I, Mice

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