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A three-dimensional collagen construct to model lipopolysaccharide-induced activation of BV2 microglia
Open accessAbstract
BACKGROUND: We report a novel method of culturing microglia in three dimension (3D) using collagen as a substrate. By culturing microglia within a matrix, we aim to emulate the physical state of microglia embedded within parenchyma. METHODS: BV2 microglia cell suspensions were prepared with type I collagen and cast into culture plates. To characterise the BV2 microglia cultured in 3D, the cultures were evaluated for their viability, cell morphology and response to lipopolysaccharide (LPS) activation. Conventional monolayer cultures (grown on uncoated and collagen-coated polystyrene) were set up concurrently for comparison. RESULTS: BV2 microglia in 3D collagen matrices were viable at 48 hrs of culture and exhibit a ramified morphology with multiplanar cytoplasmic projections. Following stimulation with 1 μg/ml LPS, microglia cultured in 3D collagen gels increase their expression of nitric oxide (NO) and CD40, indicating their capacity to become activated within the matrix. Up to 97.8% of BV2 microglia grown in 3D cultures gained CD40 positivity in response to LPS, compared to approximately 60% of cells grown in a monolayer (P<.05). BV2 microglia in 3D collagen gels also showed increased mRNA and protein expression of inflammatory cytokines IL-6, TNF-α and the chemoattractant MCP-1 following LPS stimulation. CONCLUSIONS: In summary, BV2 microglia cultured in 3D collagen hydrogels exhibit multiplanar cytoplasmic projections and undergo a characteristic and robust activation response to LPS. This culture system is accessible to a wide range of analyses and provides a useful new in vitro tool for research into microglial activation.
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3D Matrix hydrogels support microglia to grow and maintain ramified morphology and enable microglial response tests to proinflammation stimulus.
“For example, 3D Matrix hydrogels were reported to support microglia to grow and maintain ramified morphology and enable the microglial response tests to proinflammation stimulus.24, 25”
From Acoustofluidic assembly of 3D neurospheroids to model Alzheimer's disease (2020) · cites this paper as [25]
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Cites
Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo
Nimmerjahn, A., Kirchhoff, F., & Helmchen, F. (2005) · Science · doi:10.1126/science.1110647 · Cited by 5,827
Fate Mapping Analysis Reveals That Adult Microglia Derive from Primitive Macrophages
Ginhoux, F., Greter, M., Leboeuf, M., Nandi, S., See, P., Gokhan, S., Mehler, M. F., Conway, S. J., Ng, L. G., Stanley, E. R., et al. (2010) · Science · doi:10.1126/science.1194637 · Cited by 5,175
Synaptic Pruning by Microglia Is Necessary for Normal Brain Development
Paolicelli, R. C., Bolasco, G., Pagani, F., Maggi, L., Scianni, M., Panzanelli, P., Giustetto, M., Ferreira, T. A., Guiducci, E., Dumas, L., et al. (2011) · Science · doi:10.1126/science.1202529 · Cited by 4,244
Microglia Sculpt Postnatal Neural Circuits in an Activity and Complement-Dependent Manner
Schafer, D. P., Lehrman, E. K., Kautzman, A. G., Koyama, R., Mardinly, A. R., Yamasaki, R., Ransohoff, R. M., Greenberg, M. E., Barres, B. A., & Stevens, B. (2012) · Neuron · doi:10.1016/j.neuron.2012.03.026 · Cited by 4,089
Kettenmann, H., Hanisch, U.-K., Noda, M., & Verkhratsky, A. (2011) · Physiological Reviews · doi:10.1152/physrev.00011.2010 · Cited by 3,547
Microglia Shape Adult Hippocampal Neurogenesis through Apoptosis-Coupled Phagocytosis
Sierra, A., Encinas, J. M., Deudero, J. J. P., Chancey, J. H., Enikolopov, G., Overstreet-Wadiche, L. S., Tsirka, S. E., & Maletic-Savatic, M. (2010) · Cell Stem Cell · doi:10.1016/j.stem.2010.08.014 · Cited by 1,622
Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent pathways
Kierdorf, K., Erny, D., Goldmann, T., Sander, V., Schulz, C., Perdiguero, E. G., Wieghofer, P., Heinrich, A., Riemke, P., Hölscher, C., et al. (2013) · Nature Neuroscience · doi:10.1038/nn.3318 · Cited by 1,457
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Olson, J. K., & Miller, S. D. (2004) · The Journal of Immunology · doi:10.4049/jimmunol.173.6.3916 · Cited by 1,215
Immortalization of murine microglial cells by a v-raf / v-myc carrying retrovirus
Blasi, E., Barluzzi, R., Bocchini, V., Mazzolla, R., & Bistoni, F. (1990) · Journal of Neuroimmunology · doi:10.1016/0165-5728(90)90073-v · Cited by 1,075
The myeloid cells of the central nervous system parenchyma
Ransohoff, R. M., & Cardona, A. E. (2010) · Nature · doi:10.1038/nature09615 · Cited by 804
Mesenchymal stem cell–educated macrophages: A novel type of alternatively activated macrophages
Kim, J., & Hematti, P. (2009) · Experimental Hematology · doi:10.1016/j.exphem.2009.09.004 · Cited by 785
Krabbe, G., Halle, A., Matyash, V., Rinnenthal, J. L., Eom, G. D., Bernhardt, U., Miller, K. R., Prokop, S., Kettenmann, H., & Heppner, F. L. (2013) · PLoS ONE · doi:10.1371/journal.pone.0060921 · Cited by 478 · cited 1×
“Brain slice culture allows in situ examination of cellular responses, and has been used to study microglial responses to carcinomas [10] and cerebral amyloidosis [11].”
BackgroundInflammatory Neurodegeneration Mediated by Nitric Oxide, Glutamate, and Mitochondria
Brown, G. C., & Bal‐Price, A. (2003) · Molecular Neurobiology · doi:10.1385/mn:27:3:325 · Cited by 467
Swinnen, N., Smolders, S., Ávila, A., Notelaers, K., Paesen, R., Ameloot, M. M., Brône, B., Legendre, P., & Rigo, J. (2012) · Glia · doi:10.1002/glia.22421 · Cited by 226
Retinoic acid inhibits expression of TNF-? and iNOS in activated rat microglia
Dheen, S. T., Jun, Y., Yan, Z., Tay, S. S. W., & Ling, E. (2004) · Glia · doi:10.1002/glia.20153 · Cited by 205
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Lizárraga, F., Poincloux, R., Romao, M., Montagnac, G., Dez, G. L., Bonne, I., Rigaill, G. J., Raposo, G., & Chavrier, P. (2009) · Cancer Research · doi:10.1158/0008-5472.can-08-3709 · Cited by 189
Loane, D. J., Stoica, B. A., Pajoohesh‐Ganji, A., Byrnes, K. R., & Faden, A. I. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m806139200 · Cited by 121
East, E., de Oliveira, D. B., Golding, J. P., & Phillips, J. B. (2010) · Tissue Engineering Part A · doi:10.1089/ten.tea.2010.0017 · Cited by 114
Self-citationA versatile 3D culture model facilitates monitoring of astrocytes undergoing reactive gliosis
East, E., Golding, J. P., & Phillips, J. B. (2009) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.209 · Cited by 106
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Brown, R. A. (2013) · Experimental Cell Research · doi:10.1016/j.yexcr.2013.07.001 · Cited by 83
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Chuang, H., van Rossum, D., Sieger, D., Siam, L., Klemm, F., Bleckmann, A., Bayerlová, M., Farhat, K., Scheffel, J., Schulz, M., et al. (2013) · Glia · doi:10.1002/glia.22518 · Cited by 74
Gene Network Disruptions and Neurogenesis Defects in the Adult Ts1Cje Mouse Model of Down Syndrome
Hewitt, C., Ling, K., Merson, T. D., Simpson, K., Ritchie, M. E., King, S. L., Pritchard, M. A., Smyth, G. K., Thomas, T., Scott, H. S., et al. (2010) · PLoS ONE · doi:10.1371/journal.pone.0011561 · Cited by 57
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Ooi, Y. Y., Ramasamy, R., Rahmat, Z., Subramaiam, H., Tan, S. W., Abdullah, M., Israf, D. A., & Vidyadaran, S. (2010) · International Immunopharmacology · doi:10.1016/j.intimp.2010.09.001 · Cited by 55
Self-citationRahmat, Z., Jose, S., Ramasamy, R., & Vidyadaran, S. (2013) · Stem Cell Research & Therapy · doi:10.1186/scrt160 · Cited by 40
Self-citationMicro-structured Materials and Mechanical Cues in 3D Collagen Gels
Phillips, J. B., & Brown, R. A. (2010) · Methods in molecular biology · doi:10.1007/978-1-60761-984-0_12 · Cited by 37
Self-citationVidyadaran, S., Ooi, Y. Y., Subramaiam, H., Badiei, A., Abdullah, M., Ramasamy, R., & Seow, H. F. (2009) · Cellular Immunology · doi:10.1016/j.cellimm.2009.06.005 · Cited by 11
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Watson, P. M. D., Kavanagh, E., Allenby, G., & Vassey, M. (2017) · SLAS Discovery · doi:10.1177/2472555217691450 · Cited by 74
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Cuní‐López, C., Stewart, R., Oikari, L. E., Nguyen, T. H., Roberts, T. L., Sun, Y., Guo, C. C., Lupton, M. K., White, A. R., & Quek, H. (2024) · Figshare · doi:10.6084/m9.figshare.26681788
Cuní‐López, C., Stewart, R., Oikari, L. E., Nguyen, T. H., Roberts, T. L., Sun, Y., Guo, C. C., Lupton, M. K., White, A. R., & Quek, H. (2024) · Figshare · doi:10.6084/m9.figshare.25237386
Cuní‐López, C., Stewart, R., Oikari, L. E., Nguyen, T. H., Roberts, T. L., Sun, Y., Guo, C. C., Lupton, M. K., White, A. R., & Quek, H. (2024) · Figshare · doi:10.6084/m9.figshare.26681785
Cuní‐López, C., Stewart, R., Oikari, L. E., Nguyen, T. H., Roberts, T. L., Sun, Y., Guo, C. C., Lupton, M. K., White, A. R., & Quek, H. (2024) · Figshare · doi:10.6084/m9.figshare.26681791
Cuní‐López, C., Stewart, R., Oikari, L. E., Nguyen, T. H., Roberts, T. L., Sun, Y., Guo, C. C., Lupton, M. K., White, A. R., & Quek, H. (2024) · Figshare · doi:10.6084/m9.figshare.26681779
Cuní‐López, C., Stewart, R., Oikari, L. E., Nguyen, T. H., Roberts, T. L., Sun, Y., Guo, C. C., Lupton, M. K., White, A. R., & Quek, H. (2024) · Figshare · doi:10.6084/m9.figshare.26681782
Metadata
- Authors
- Haw, R. T. Y., Tong, C. K., Yew, A., Lee, H. C., Phillips, J. B., & Vidyadaran, S.
- Affiliations
- Universiti Putra Malaysia (MY)
- University College London (GB)
- Corresponding author
- Vidyadaran, Sharmili
- Venue
- Journal of Neuroinflammation
- Published
- 2014-07-30
- Volume
- 11
- Issue
- 1
- Pages
- 134-134
- ISSN
- 1742-2094 (ISSN-L)
- PMC
- PMC4128540
- OpenAlex
- W2109774877
- Cited by
- 33
- References
- 26
- Citations by year
- 2025: 1 · 2024: 9 · 2023: 4 · 2022: 1 · 2021: 7 · 2020: 3 · 2018: 2 · 2017: 4 · 2016: 1 · 2015: 1
- FWCI
- 1.04
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- cc-by
- Locations
- Journal of Neuroinflammation (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Universiti Putra Malaysia Institutional Repository (Universiti Putra Malaysia) (acceptedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Greater South Information System
- Funders
- Monash University
- Universiti Putra Malaysia
- Ministry of Education, India
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Neuroscience › Neurology › Neurological Disease Mechanisms and Treatments
- Keywords
- BV-2 cells, microglial activation, lipopolysaccharide, type I collagen, microglial morphology, nitric oxide, CD40, inflammatory cytokines, interleukin-6, TNF-α, MCP-1
- MeSH terms
- Animals, Cell Line, Transformed, Collagen, L-Lactate Dehydrogenase, Lipopolysaccharides, Microscopy, Electron, Scanning, Models, Biological, Cytokines, Microglia, CD40 Antigens, Imaging, Three-Dimensional, Lectins, Mice
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