Characterization of sustained BOLD activation in the rat barrel cortex and neurochemical consequences
Open accessAbstract
To date, only a couple of functional MR spectroscopy (fMRS) studies were conducted in rats. Due to the low temporal resolution of (1)H MRS techniques, prolonged stimulation paradigms are necessary for investigating the metabolic outcome in the rat brain during functional challenge. However, sustained activation of cortical areas is usually difficult to obtain due to neural adaptation. Anesthesia, habituation, high variability of the basal state metabolite concentrations as well as low concentrations of the metabolites of interest such as lactate (Lac), glucose (Glc) or γ-aminobutyric acid (GABA) and small expected changes of metabolite concentrations need to be addressed. In the present study, the rat barrel cortex was reliably and reproducibly activated through sustained trigeminal nerve (TGN) stimulation. In addition, TGN stimulation induced significant positive changes in lactate (+1.01 μmol/g, p<0.008) and glutamate (+0.92 μmol/g, p<0.02) and significant negative aspartate changes (-0.63 μmol/g, p<0.004) using functional (1)H MRS at 9.4 T in agreement with previous changes observed in human fMRS studies. Finally, for the first time, the dynamics of lactate, glucose, aspartate and glutamate concentrations during sustained somatosensory activation in rats using fMRS were assessed. These results allow demonstrating the feasibility of fMRS measurements during prolonged barrel cortex activation in rats.
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Prolonged (i.e., minutes) stimulations elicit a persistent BOLD response with minimal attenuation in humans and rats.
“Nonetheless, results in both humans (Mangia et al., 2007b) and rats (Just et al., 2013) have shown that prolonged (i.e., minutes) stimulations elicit a persistent BOLD response with minimal attenuation.”
From Perception is associated with the brain’s metabolic response to sensory stimulation (2022) · cites this paper as Just et al., 2013
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Self-citation
- Authors
- Just, N., Xin, L., Frenkel, H., & Gruetter, R.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Corresponding author
- Just, Nathalie
- Venue
- NeuroImage
- Publisher
- Elsevier BV
- Published
- 2013-07
- Volume
- 74
- Pages
- 343-351
- ISSN
- 1053-8119 (ISSN-L)
- OpenAlex
- W2163130791
- Cited by
- 35
- References
- 33
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 1 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 1 · 2017: 5 · 2016: 1 · 2015: 4 · 2014: 3 · 2013: 2
- FWCI
- 2.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- NeuroImage (publishedVersion)
- PubMed (publishedVersion)
- Archive ouverte UNIGE (University of Geneva) (publishedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- IRIS (submittedVersion)
- SERVAL (Université de Lausanne) (submittedVersion)
- Archive ouverte UNIGE (University of Geneva) (submittedVersion, cc-by)
- Funders
- Centre d'Imagerie BioMédicale (CIBM) of Ecole Polytechnique Fédérale de Lausanne (EPFL)
- University of Lausanne (UNIL)
- Foundations Leenards et Jeantet
- Topics
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced Neuroimaging Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- barrel cortex, trigeminal nerve stimulation, lactate, glutamate, aspartic acid, glucose, brain metabolites, neural adaptation, rat brain
- MeSH terms
- Animals, Brain Mapping, Electric Stimulation, Male, Magnetic Resonance Spectroscopy, Somatosensory Cortex, Trigeminal Nerve, Vibrissae, Rats, Sprague-Dawley, Rats
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