MAP2 defines a pre-axonal filtering Zone to regulate KIF1- versus KIF5-Dependent cargo transport in sensory neurons
Open accessAbstract
Polarized cargo transport is essential for neuronal function. However, the minimal basic components required for selective cargo sorting and distribution in neurons remain elusive. We found that in sensory neurons the axon initial segment is largely absent and that microtubule-associated protein 2 (MAP2) defines the cargo-filtering zone in the proximal axon. Here, MAP2 directs axonal cargo entry by coordinating the activities of molecular motors. We show that distinct kinesins differentially regulate cargo velocity: kinesin-3 drives fast axonal cargo trafficking, while kinesin-1 slows down axonal cargo transport. MAP2 inhibits "slow" kinesin-1 motor activity and allows kinesin-3 to drive robust cargo transport from the soma into the axon. In the distal axon, the inhibitory action of MAP2 decreases, leading to regained kinesin-1 activity and vesicle distribution. We propose that selective axonal cargo trafficking requires the MAP2-defined pre-axonal filtering zone and the ability of cargos to switch between distinct kinesin motor activities.
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MAP2 has been shown to bind to motor proteins in neurons.
“Since MAP2 has been shown to bind to motor proteins in neurons (13)”
From Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons (2026) · cites this paper as [13]
The “MAP2 Interacts with the Coiled-Coil Region of KIF5” section reports that MAP2 co-precipitated with the motor protein KIF5, but that binding experiment used HEK293 cells, not neurons. Experiments in DRG neurons support an interaction between MAP2 and KIF5, but do not directly demonstrate binding in neurons. (LLM, conf=0.84)
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Cites
Neurofascin assembles a specialized extracellular matrix at the axon initial segment
Hedstrom, K. L., Xu, X., Ogawa, Y., Frischknecht, R., Seidenbecher, C. I., Shrager, P., & Rasband, M. N. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200705119 · Cited by 268 · see citing works
van Beuningen, S. F. B., Will, L., Harterink, M., Chazeau, A., van Battum, E. Y., Frias, C. P., Franker, M. A. M., Katrukha, E. A., Stucchi, R., Vocking, K., et al. (2015) · Neuron · doi:10.1016/j.neuron.2015.11.012 · Cited by 225 · see citing works
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Research works printed but not linked: 2
- Martini, F.H. (2011). Fundamentals of Anatomy and Physiology, Ninth Edition (Pearson Education).
- Taylor, J.R. (1997). An Introduction to Error Analysis: The Study of Uncertainties in Physical Measurements, 2nd Edition (University Science Books).
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The Role of the Microtubule Cytoskeleton in Neurodevelopmental Disorders
Lasser, M., Tiber, J., & Lowery, L. A. (2018) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2018.00165 · Cited by 201 · see citing works
Tau Protein Interaction Partners and Their Roles in Alzheimer’s Disease and Other Tauopathies
Sinsky, J., Pichlerova, K., & Hanes, J. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22179207 · Cited by 220 · see citing works
A combinatorial MAP code dictates polarized microtubule transport
Monroy, B. Y., Tan, T. C., Oclaman, J. M., Han, J. S., Simó, S., Niwa, S., Nowakowski, D. W., McKenney, R. J., & Ori-McKenney, K. M. (2020) · Developmental Cell · doi:10.1016/j.devcel.2020.01.029 · Cited by 157 · see citing works
Microtubule lattice spacing governs cohesive envelope formation of tau family proteins
Siahaan, V., Tan, R., Humhalova, T., Libusova, L., Lacey, S. E., Tan, T., Dacy, M., Ori-McKenney, K. M., McKenney, R. J., Braun, M., et al. (2022) · Nature Chemical Biology · doi:10.1038/s41589-022-01096-2 · Cited by 94 · see citing works
Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons
Yonemura, Y., Sakai, Y., Nakata, R., Hagita-Tatsumoto, A., Miyasaka, T., & Misonou, H. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.113407
- Authors
- Gumy, L. F., Katrukha, E. A., Grigoriev, I., Jaarsma, D., Kapitein, L. C., Akhmanova, A., & Hoogenraad, C. C.
- Affiliations
- Utrecht University (NL)
- Erasmus MC (NL)
- Corresponding author
- Hoogenraad, Casper C.
- ORCID
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2017-04
- Volume
- 94
- Issue
- 2
- Pages
- 347-362.e7
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W2607503492
- Cited by
- 186
- References
- 2
- Citations by year
- 2026: 13 · 2025: 21 · 2024: 18 · 2023: 12 · 2022: 20 · 2021: 23 · 2020: 25 · 2019: 28 · 2018: 20 · 2017: 6
- FWCI
- 11.53
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Utrecht University Repository (Utrecht University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Hereditary Neurological Disorders
- Keywords
- MAP2, sensory neurons, kinesin-1, kinesin-3, kinesin-5, axon initial segment, cargo sorting, molecular motors, microtubule-associated proteins
- MeSH terms
- Animals, Axons, Axonal Transport, Cell Line, Cells, Cultured, Dendrites, Microtubule-Associated Proteins, Microtubules, Models, Biological, Sensory Receptor Cells, Kinesins, Rats
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