Imaging neurotransmitter and neuromodulator dynamics in vivo with genetically encoded indicators
Open accessAbstract
The actions of neuromodulation are thought to mediate the ability of the mammalian brain to dynamically adjust its functional state in response to changes in the environment. Altered neurotransmitter (NT) and neuromodulator (NM) signaling is central to the pathogenesis or treatment of many human neurological and psychiatric disorders, including Parkinson's disease, schizophrenia, depression, and addiction. To reveal the precise mechanisms by which these neurochemicals regulate healthy and diseased neural circuitry, one needs to measure their spatiotemporal dynamics in the living brain with great precision. Here, we discuss recent development, optimization, and applications of optical approaches to measure the spatial and temporal profiles of NT and NM release in the brain using genetically encoded sensors for in vivo studies.
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Intersectional genetic and viral tools allow manipulation of specific neuronal populations suspected of neurotransmitter corelease and enable functional analysis of cotransmitting neurons in vitro and in vivo.
“new ''intersectional'' genetic and viral tools permit manipulation of specific neuronal populations suspected of neurotransmitter corelease 10 and enable functional analysis of cotransmitting neurons in vitro and in vivo.11,12”
From Synaptic and circuit functions of multitransmitter neurons in the mammalian brain (2023) · cites this paper as [12]
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Self-citation
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Neural circuits regulating prosocial behaviors
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Synaptic and circuit functions of multitransmitter neurons in the mammalian brain
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Self-citationA connectome is not enough – what is still needed to understand the brain of<i>Drosophila</i>?
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Ventral striatum dopamine release encodes unique properties of visual stimuli in mice
Gonzalez, L. S., Fisher, A. A., D'Souza, S. P., Cotella, E. M., Lang, R. A., & Robinson, J. E. (2023) · eLife · doi:10.7554/elife.85064 · Cited by 18 · see citing works
“85064 7 of 20 neural activity, structure, or biology (Fenno et al., 2011; Resendez and Stuber, 2015; Sabatini and Tian, 2020).”
BackgroundAnalytical approaches to examine gamma-aminobutyric acid and glutamate vesicular co-packaging
Kim, S., & Sabatini, B. L. (2023) · Frontiers in Synaptic Neuroscience · doi:10.3389/fnsyn.2022.1076616 · Cited by 10 · see citing works
Self-citation
- Authors
- Sabatini, B. L., & Tian, L.
- Affiliations
- Howard Hughes Medical Institute (US)
- University of California, Davis (US)
- Corresponding author
- Sabatini, Bernardo L.
- Tian, Lin
- Venue
- Neuron
- Publisher
- Elsevier BV
- Published
- 2020-10
- Volume
- 108
- Issue
- 1
- Pages
- 17-32
- ISSN
- 0896-6273 (ISSN-L)
- OpenAlex
- W3092815585
- Cited by
- 203
- References
- 144
- Citations by year
- 2026: 27 · 2025: 22 · 2024: 33 · 2023: 34 · 2022: 46 · 2021: 40 · 2020: 1
- FWCI
- 3.79
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Neuron (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Harvard Medical School
- University of California, Davis
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
- Keywords
- neurotransmitter release, neuromodulation, genetically encoded indicators, optogenetics, in vivo imaging, spatiotemporal dynamics, Parkinson's disease, schizophrenia, depression, addiction, neural circuits
- MeSH terms
- Animals, Brain, Humans, Neurons, Protein Engineering, Biosensing Techniques, Neurotransmitter Agents, Periplasmic Binding Proteins, Receptors, G-Protein-Coupled, Optical Imaging, Optogenetics
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