Cited by 203Advanced Fluorescence Microscopy TechniquesNeuroscience and Neuropharmacology ResearchNeurotransmitter Receptor Influence on Behavior

Imaging neurotransmitter and neuromodulator dynamics in vivo with genetically encoded indicators

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Sabatini, Bernardo L., Tian, Lin·2020·Neuron

Abstract

The actions of neuromodulation are thought to mediate the ability of the mammalian brain to dynamically adjust its functional state in response to changes in the environment. Altered neurotransmitter (NT) and neuromodulator (NM) signaling is central to the pathogenesis or treatment of many human neurological and psychiatric disorders, including Parkinson's disease, schizophrenia, depression, and addiction. To reveal the precise mechanisms by which these neurochemicals regulate healthy and diseased neural circuitry, one needs to measure their spatiotemporal dynamics in the living brain with great precision. Here, we discuss recent development, optimization, and applications of optical approaches to measure the spatial and temporal profiles of NT and NM release in the brain using genetically encoded sensors for in vivo studies.

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Crossref · 10.1016/j.neuron.2020.09.036

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144 of 144 references verified0 citation errors · 0 not checkable

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  • Intersectional genetic and viral tools allow manipulation of specific neuronal populations suspected of neurotransmitter corelease and enable functional analysis of cotransmitting neurons in vitro and in vivo.

    “new ''intersectional'' genetic and viral tools permit manipulation of specific neuronal populations suspected of neurotransmitter corelease 10 and enable functional analysis of cotransmitting neurons in vitro and in vivo.11,12”

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Authors
Sabatini, B. L., & Tian, L.
Affiliations
  • Howard Hughes Medical Institute (US)
  • University of California, Davis (US)
Corresponding author
  • Sabatini, Bernardo L.
  • Tian, Lin
Venue
Neuron
Publisher
Elsevier BV
Published
2020-10
Volume
108
Issue
1
Pages
17-32
ISSN
0896-6273 (ISSN-L)
OpenAlex
W3092815585
Cited by
203
References
144
Citations by year
2026: 27 · 2025: 22 · 2024: 33 · 2023: 34 · 2022: 46 · 2021: 40 · 2020: 1
FWCI
3.79
Type
journal article
Language
en
Open access
Locations
  • Neuron (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Harvard Medical School
  • University of California, Davis
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neurotransmitter Receptor Influence on Behavior
Keywords
neurotransmitter release, neuromodulation, genetically encoded indicators, optogenetics, in vivo imaging, spatiotemporal dynamics, Parkinson's disease, schizophrenia, depression, addiction, neural circuits
MeSH terms
Animals, Brain, Humans, Neurons, Protein Engineering, Biosensing Techniques, Neurotransmitter Agents, Periplasmic Binding Proteins, Receptors, G-Protein-Coupled, Optical Imaging, Optogenetics

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